Heat Shock Proteins: Classification, Functions and Expressions in Plants during Environmental Stresses.

Khan, Sara; Jabeen, Raheela; Deeba, Farah; Waheed, Ummara; Khanum, Plosha; Iqbal, Nadeem

doi:10.35691/jbm.1202.0183

Cited by 14 publications

(9 citation statements)

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“…StMAPKK5 overexpression significantly enhances the mRNA expression of SOD, catalase and peroxidase responding to heat stress. HSFA protein activate HSP expression, which are molecular chaperones that assist in protein refolding and prevent protein denaturation under heat stress conditions ( Bourgine and Guihur, 2021 ; Khan et al., 2021 ). From our results, the increase in StHSFA3 mRNA expression and the induction of StHsp20–20 , StHsp20–33 , StHsp20–44 , StHsp70 , StHsp90.2 , and StHsp90.4 seems to be related to StMAPKK5 overexpression.…”

Section: Discussionmentioning

confidence: 99%

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

Zhu,

Li,

Zhang

et al. 2024

Front. Plant Sci.

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BackgroundsAs a conserved signaling pathway, mitogen-activated protein kinase (MAPK) cascade regulates cellular signaling in response to abiotic stress. High temperature may contribute to a significant decrease in economic yield. However, research into the expression patterns of StMAPKK family genes under high temperature is limited and lacks experimental validation regarding their role in supporting potato plant growth.MethodsTo trigger heat stress responses, potato plants were grown at 35°C. qRT-PCR was conducted to analyze the expression pattern of StMAPKK family genes in potato plants. Plant with StMAPKK5 loss-of-function and gain-of-function were developed. Potato growth and morphological features were assessed through measures of plant height, dry weight, and fresh weight. The antioxidant ability of StMAPKK5 was indicated by antioxidant enzyme activity and H2O2 content. Cell membrane integrity and permeability were suggested by relative electrical conductivity (REC), and contents of MDA and proline. Photosynthetic capacity was next determined. Further, mRNA expression of heat stress-responsive genes and antioxidant enzyme genes was examined.ResultsIn reaction to heat stress, the expression profiles of StMAPKK family genes were changed. The StMAPKK5 protein is located to the nucleus, cytoplasm and cytomembrane, playing a role in controlling the height and weight of potato plants under heat stress conditions. StMAPKK5 over-expression promoted photosynthesis and maintained cell membrane integrity, while inhibited transpiration and stomatal conductance under heat stress. Overexpression of StMAPKK5 triggered biochemical defenses in potato plant against heat stress, modulating the levels of H2O2, MDA and proline, as well as the antioxidant activities of CAT, SOD and POD. Overexpression of StMAPKK5 elicited genetic responses in potato plants to heat stress, affecting heat stress-responsive genes and genes encoding antioxidant enzymes.ConclusionStMAPKK5 can improve the resilience of potato plants to heat stress-induced damage, offering a promising approach for engineering potatoes with enhanced adaptability to challenging heat stress conditions.

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Section: Discussionmentioning

confidence: 99%

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

Zhu,

Li,

Zhang

et al. 2024

Front. Plant Sci.

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“…The HS signal perception by Hsfs leads to an increased expression of several Hsps. These are essential in maintaining balanced cell internal conditions under optimum and stress conditions and their main functions involve protein folding, unfolding and transport, thus maintaining plant homeostasis ( Khan et al., 2021 ). Hsps are generally grouped into five classes based on their molecular weight in kilo Dalton (kDa), such as Hsp100, Hsp90, Hsp70, Hsp60 and small Hsps (sHsps) ( Wang et al., 2004 ; Kotak et al., 2007 ; ul Haq et al., 2019 ) ( Table 2 ).…”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Tomato plant response to heat stress: a focus on candidate genes for yield-related traits

Graci,

Barone

2024

Front. Plant Sci.

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Climate change and global warming represent the main threats for many agricultural crops. Tomato is one of the most extensively grown and consumed horticultural products and can survive in a wide range of climatic conditions. However, high temperatures negatively affect both vegetative growth and reproductive processes, resulting in losses of yield and fruit quality traits. Researchers have employed different parameters to evaluate the heat stress tolerance, including evaluation of leaf- (stomatal conductance, net photosynthetic rate, Fv/Fm), flower- (inflorescence number, flower number, stigma exertion), pollen-related traits (pollen germination and viability, pollen tube growth) and fruit yield per plant. Moreover, several authors have gone even further, trying to understand the plants molecular response mechanisms to this stress. The present review focused on the tomato molecular response to heat stress during the reproductive stage, since the increase of temperatures above the optimum usually occurs late in the growing tomato season. Reproductive-related traits directly affects the final yield and are regulated by several genes such as transcriptional factors, heat shock proteins, genes related to flower, flowering, pollen and fruit set, and epigenetic mechanisms involving DNA methylation, histone modification, chromatin remodelling and non-coding RNAs. We provided a detailed list of these genes and their function under high temperature conditions in defining the final yield with the aim to summarize the recent findings and pose the attention on candidate genes that could prompt on the selection and constitution of new thermotolerant tomato plant genotypes able to face this abiotic challenge.

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“…For example, they buffer and bind to the hydrophobic regions of unfolded polypeptides during translation, preventing aggregation and amino-terminal misfolding and ensuring proper folding of the polypeptide chain (Roy et al 2019 ). Additionally, they assist in stabilizing protein structure, maintaining normal conformation, and regulating cellular homeostasis (Khan et al 2021 ). Using SDS-PAGE, isoelectric focusing (IEF), western blot, and dot blot techniques, Polenta et al ( 2020 ) discovered an increased expression of HSP in tomatoes in response to extreme heat and cold conditions.…”

Section: Heat Shock Proteins As a Biochemical Biomarker In Plant Stre...mentioning

confidence: 99%

Plant biomarkers as early detection tools in stress management in food crops: a review

Aina,

Bakare,

Fadaka

et al. 2024

Planta

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Main conclusion Plant Biomarkers are objective indicators of a plant’s cellular state in response to abiotic and biotic stress factors. They can be explored in crop breeding and engineering to produce stress-tolerant crop species. Abstract Global food production safely and sustainably remains a top priority to feed the ever-growing human population, expected to reach 10 billion by 2050. However, abiotic and biotic stress factors negatively impact food production systems, causing between 70 and 100% reduction in crop yield. Understanding the plant stress responses is critical for developing novel crops that can adapt better to various adverse environmental conditions. Using plant biomarkers as measurable indicators of a plant’s cellular response to external stimuli could serve as early warning signals to detect stresses before severe damage occurs. Plant biomarkers have received considerable attention in the last decade as pre-stress indicators for various economically important food crops. This review discusses some biomarkers associated with abiotic and biotic stress conditions and highlights their importance in developing stress-resilient crops. In addition, we highlighted some factors influencing the expression of biomarkers in crop plants under stress. The information presented in this review would educate plant researchers, breeders, and agronomists on the significance of plant biomarkers in stress biology research, which is essential for improving plant growth and yield toward sustainable food production.

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Heat Shock Proteins: Classification, Functions and Expressions in Plants during Environmental Stresses.

Cited by 14 publications

References 0 publications

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

StMAPKK5 responds to heat stress by regulating potato growth, photosynthesis, and antioxidant defenses

Tomato plant response to heat stress: a focus on candidate genes for yield-related traits

Plant biomarkers as early detection tools in stress management in food crops: a review

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