Heat shock proteins: a therapeutic target worth to consider

Dubey, Amita; Prajapati, K. S.; Swamy, Madhu; Pachauri, Vivekin

doi:10.14202/vetworld.2015.46-51

Cited by 46 publications

(30 citation statements)

References 59 publications

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“…Heat shock protein genes ( HSPA5, HSPA6, HSPA8, HSP90AA1, HSP 70.1 ), found between the highly expressed genes in the lactation, have a protective function, allowing cells to survive lethal conditions, and also interact with various components of the programmed cell death machinery; on the other hand, membrane bound HSPs mediate immunological functions [97, 98]. Our finding confirms what reported in literature about HSP functions and demonstrates how MSCs, in goat colostrum, can support a high level of protein synthesis act to operate in both immunity and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

Crisà

Ferré

Chillemi³

et al. 2016

BMC Vet Res

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BackgroundIn this work we aimed at sequencing and assembling the goat milk transcriptome corresponding at colostrum and 120 days of lactation. To reconstruct transcripts we used both the genome as reference, and a de novo assembly approach. Additionally, we aimed at identifying the differentially expressed genes (DEGs) between the two lactation stages and at analyzing the expression of genes involved in oligosaccharides metabolism.ResultsA total of 44,635 different transcripts, organized in 33,757 tentative genes, were obtained using the goat genome as reference. A significant sequence similarity match was found for 40,353 transcripts (90%) against the NCBI NT and for 35,701 (80%) against the NR databases. 68% and 69% of the de novo assembled transcripts, in colostrum and 120 days of lactation samples respectively, have a significant match with the merged transcriptome obtained using Cufflinks/Cuffmerge. CSN2, PAEP, CSN1S2, CSN3, LALBA, TPT1, FTH1, M-SAA3, SPP1, GLYCAM1, EEF1A1, CTSD, FASN, RPS29, CSN1S1, KRT19 and CHEK1 were found between the top fifteen highly expressed genes. 418 loci were differentially expressed between lactation stages, among which 207 and 122 were significantly up- and down-regulated in colostrum, respectively. Functional annotation and pathway enrichment analysis showed that in goat colostrum somatic cells predominate biological processes involved in glycolysis, carbohydrate metabolism, defense response, cytokine activity, regulation of cell proliferation and cell death, vasculature development, while in mature milk, biological process associated with positive regulation of lymphocyte activation and anatomical structure morphogenesis are enriched. The analysis of 144 different oligosaccharide metabolism-related genes showed that most of these (64%) were more expressed in colostrum than in mature milk, with eight expressed at very high levels (SLCA3, GMSD, NME2, SLC2A1, B4GALT1, B3GNT2, NANS, HEXB).ConclusionsTo our knowledge, this is the first study comparing goat transcriptome of two lactation stages: colostrum and 120 days. Our findings suggest putative differences of expression between stages and can be envisioned as a base for further research in the topic. Moreover because a higher expression of genes involved in immune defense response, carbohydrate metabolism and related to oligosaccharide metabolism was identified in colostrum we here corroborate the potential of goat milk as a natural source of lactose-derived oligosaccharides and for the development of functional foods.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-016-0881-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

Crisà

Ferré

Chillemi³

et al. 2016

BMC Vet Res

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“…Additionally, increased expression of heat shock protein 70 (HSP70) is seen in the ischemic penumbra, signifying its role in the attenuation and protection of ischemia. Dubey et al, using cerebral ischemia mice models, showed overexpressing HSP70 protected against myocardial and cerebral ischemia (MCA occlusion); preconditioning with ischemia showed enhanced expression of HSP70 in the cell ( 113 ).…”

Section: Mechanisms Of Limb Remote Ischemic Pre- Per- and Postcondimentioning

confidence: 99%

Limb Remote Ischemic Conditioning: Mechanisms, Anesthetics, and the Potential for Expanding Therapeutic Options

et al. 2018

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Novel and innovative approaches are essential in developing new treatments and improving clinical outcomes in patients with ischemic stroke. Remote ischemic conditioning (RIC) is a series of mechanical interruptions in blood flow of a distal organ, following end organ reperfusion, shown to significantly reduce infarct size through inhibition of oxidation and inflammation. Ischemia/reperfusion (I/R) is what ultimately leads to the irreversible brain damage and clinical picture seen in stroke patients. There have been several reports and reviews about the potential of RIC in acute ischemic stroke; however, the focus here is a comprehensive look at the differences in the three types of RIC (remote pre-, per-, and postconditioning). There are some limited uses of preconditioning in acute ischemic stroke due to the unpredictability of the ischemic event; however, it does provide the identification of biomarkers for clinical studies. Remote limb per- and postconditioning offer a more promising treatment during patient care as they can be harnessed during or after the initial ischemic insult. Though further research is needed, it is imperative to discuss the importance of preclinical data in understanding the methods and mechanisms involved in RIC. This understanding will facilitate translation to a clinically feasible paradigm for use in the hospital setting.

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“…Unterstützt wird diese Annahme dadurch, dass überlebende neuronale Zellen oftmals HSPs überexprimieren. Die erhöhte Expression von HSP70 in Tiermodellen von Morbus Parkinson und Morbus Alzheimer führte durch eine Reduktion des durch Caspase-3 induzierten Zelltodes zu einer Reduktion der krankheitstypischen Symptome [45]. Für den klinischen Einsatz von protektiven HSPs gibt es bisher nur wenige Beispiele.…”

Section: Hitzeschockproteine Als Zukünftiger Therapieansatzunclassified

Derzeitiger Stand zur primären Neuroprotektion beim Glaukom

Reinehr

Grotegut

Tsai

et al. 2020

Klin Monatsbl Augenheilkd

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ZusammenfassungAngesichts der zunehmenden Alterung unserer Gesellschaft wird es in den kommenden Jahren zu einem Anstieg von starken Sehbinderungen und Erblindungen, auch durch das Glaukom, kommen. Aktuell beschränken sich die Therapieoptionen lediglich auf die Behandlung der Symptome, was im besten Fall zu einer Verlangsamung der Progression führt, jedoch nicht zur Heilung. Es ist daher notwendig, neue Therapiestrategien zu entwickeln, um das Glaukom adäquat und effektiv behandeln zu können und damit die Lebensqualität der Betroffenen zu verbessern. Ein möglicher Ansatz scheint die primäre Neuroprotektion darzustellen, die unabhängig von einer Drucksenkung wirkt. Es gibt Hinweise, dass Komponenten des Immunsystems eine Rolle im Rahmen der Erkrankung bzw. des Unterganges der retinalen Ganglienzellen spielen. So wurden Hinweise auf eine Beteiligung von Hitzeschockproteinen, des Komplementsystems, aber auch von Mikrogliazellen gefunden. Daher scheint eine therapeutische Modulation dieser Faktoren ein interessanter neuer Angriffspunkt für die Neuroprotektion zu sein. Untersuchungen im Tiermodell zeigten bspw., dass eine Inhibition des Komplementsystems oder der Mikroglia zu einem Schutz führt. Durch eine Modulation der Hitzeschockproteine könnten entweder deren protektive Eigenschaften gefördert oder ihre schädliche Funktion gehemmt werden, um so einen Glaukomschaden zu verhindern. Diese neuroprotektiven Ansätze könnten in Zukunft die Therapieoptionen von Glaukompatienten erweitern.

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Heat shock proteins: a therapeutic target worth to consider

Cited by 46 publications

References 59 publications

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

RNA-Sequencing for profiling goat milk transcriptome in colostrum and mature milk

Limb Remote Ischemic Conditioning: Mechanisms, Anesthetics, and the Potential for Expanding Therapeutic Options

Derzeitiger Stand zur primären Neuroprotektion beim Glaukom

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