Heat shock protein gene expression profile may differentiate between rheumatoid arthritis, osteoarthritis, and healthy controls

Sedláčková, Lucie; Sosna, A; Vavrincová, P; Frýdl, J; Guerriero, Vince; Raynes, Deborah A.; Hromadníková, Ilona

doi:10.3109/03009742.2011.552522

Cited by 18 publications

(14 citation statements)

References 15 publications

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“…Accumulating studies have indicated the intracellular role of Hsp90 α in tumorigenesis. In 2011, Sedlackova et al [51] detected the HSP expression profile by real-time quantitative reverse transcription polymerase chain reaction in RA, osteoarthritis, and healthy controls. Their data showed the significantly increased Hsp90 α mRNA level in RA synovial tissues.…”

Section: Resultsmentioning

confidence: 99%

A Systems Biology-Based Investigation into the Pharmacological Mechanisms of Wu Tou Tang Acting on Rheumatoid Arthritis by Integrating Network Analysis

Zhang

Wang

Tan

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Aim. To investigate pharmacological mechanisms of Wu Tou Tang acting on rheumatoid arthritis (RA) by integrating network analysis at a system level. Methods and Results. Drug similarity search tool in Therapeutic Targets Database was used to screen 153 drugs with similar structures to compositive compounds of each ingredient in Wu Tou Tang and to identify 56 known targets of these similar drugs as predicted molecules which Wu Tou Tang affects. The recall, precision, accuracy, and F1-score, which were calculated to evaluate the performance of this method, were, respectively, 0.98, 0.61, 59.67%, and 0.76. Then, the predicted effector molecules of Wu Tou Tang were significantly enriched in neuroactive ligand-receptor interaction and calcium signaling pathway. Next, the importance of these predicted effector molecules was evaluated by analyzing their network topological features, such as degree, betweenness, and k-coreness. We further elucidated the biological significance of nine major candidate effector molecules of Wu Tou Tang for RA therapy and validated their associations with compositive compounds in Wu Tou Tang by the molecular docking simulation. Conclusion. Our data suggest the potential pharmacological mechanisms of Wu Tou Tang acting on RA by combining the strategies of systems biology and network pharmacology.

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Section: Resultsmentioning

confidence: 99%

A Systems Biology-Based Investigation into the Pharmacological Mechanisms of Wu Tou Tang Acting on Rheumatoid Arthritis by Integrating Network Analysis

Zhang

Wang

Tan

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…Sedlackova L found that signi cantly increased Hsp27 and Hsp90a mRNA levels in RA synovial tissues, which indicated that Hsps could be new diagnostic approach to RA patients 30 . The anti-apoptotic activity of inhibitors of apoptosis proteins (IAP) proteins can be blocked by the SMAC, which is liberated into the cytoplasm in response to proapoptotic stimuli.…”

Section: Discussionmentioning

confidence: 99%

Using protein microarray reveals clinical correlation between self-perception of patient and the apoptosis-related proteins in rheumatoid arthritis

Wen

Liu

Jiang

et al. 2020

Preprint

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Abstract Background: The most severe effects of rheumatoid arthritis (RA) are loss of physical function, which may have a significant impact on self-perception of patient (SPP). However, the inherent relationship between SPP and the key proteins is not clear. The aim of this study was to get an insight into SPP of RA in connection with the the apoptosis-related proteins. Methods: We set out to investigate changes of the apoptosis-related proteins expression in the peripheral blood mononuclear cells (PBMCs) of RA. Additionally, we aimed to correlate the apoptosis-related proteins expression profiles with SPP and clinical indexes. To this end, we employed antibody microarrays of the the apoptosis-related proteins in PBMCs from four RA patients and seven healthy controls. We used bioinformatics to screen several the apoptosis-related proteins. To validate key protein candidates, we performed Enzyme linked immunosorbent assay (ELISA) on 30 RA patients and 30 healthy controls. Results: We found the expression of ten the apoptosis-related proteins (caspase3, CD40, SMAC, HSP27, HTRA, IGFBP-1, IGFBP-6, sTNF-R1, sTNF-R2, TRAILR-3) were significantly altered in PBMCs of RA patients. Receiver operating characteristic (ROC) curve analysis suggested that these ten the apoptosis-related proteins are potential biomarkers of RA. Spearman Correlation analysis and Logistic-regression analysis revealed that the 10 selected the apoptosis-related proteins correlated with SPP and clinical indexes. Conclusion: Therefore, we highlight some the apoptosis-related proteins may serve as potential biomarkers in prediction of SPP for RA patients, although the underlying mechanisms need to be further explored.

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“…On a 2D gel, there should be multiple HSP27 protein spots which include various levels of phosphorylation. Total expression of HSP27 may be increased in the affected sites of RA because messenger RNA (mRNA) expression of HSP27 in RA synovial tissue was increased compared to that in OA tissue . On the other hand, a DIGE study showed decreased intensity of one HSP27 spot in OA compared to that under healthy conditions, suggesting the decrease of one phosphorylated or non‐phosphorylated type of HSP27 in OA .…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of bone marrow‐adherent cells in rheumatoid arthritis and osteoarthritis

Kamada

Kurokawa²,

Kato³

et al. 2012

Int J of Rheum Dis

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Heat shock protein gene expression profile may differentiate between rheumatoid arthritis, osteoarthritis, and healthy controls

Abstract: Hsp gene expression analysis can be implemented as a new diagnostic approach to facilitate differentiation between RA, OA, and healthy controls.

Cited by 18 publications

References 15 publications

A Systems Biology-Based Investigation into the Pharmacological Mechanisms of Wu Tou Tang Acting on Rheumatoid Arthritis by Integrating Network Analysis

A Systems Biology-Based Investigation into the Pharmacological Mechanisms of Wu Tou Tang Acting on Rheumatoid Arthritis by Integrating Network Analysis

Using protein microarray reveals clinical correlation between self-perception of patient and the apoptosis-related proteins in rheumatoid arthritis

Proteomic analysis of bone marrow‐adherent cells in rheumatoid arthritis and osteoarthritis

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