Heat shock protein expression in thermotolerant and thermosensitive lines of cotton

Abstract

The expression of heat shock proteins (HSPs) was compared between genetically characterized heat tolerant and heat sensitive lines of cotton (Gossypium hirsutum andG. barbadense) using electrophoretic analysis ofin vivo labelled proteins. No differences were observed between the two lines with regard to: the temperature at which HSP synthesis was induced (37°C); the temperature at which HSP synthesis was maximal (45°C); the rates of recovery from HSP synthesis; the duration of HSP synthesis; or the major size classes of HSPs expressed in these two lines. Several HSPs were identified on 2D gels which were expressed uniquely in either the tolerant or sensitive cotton line. However, the HSP pattern displayed in a heat tolerant BC-3 individual was that of the heat sensitive parent.

Heat stress induces the synthesis of a new form of ribulose-1,5-bisphosphate carboxylase/oxygenase activase in cotton leaves

“…In a previous study, induction of heat-shock protein (HSP) synthesis in cotton leaves began at 37°C and was maximal at 45°C (Fender and O'Connell 1989), indicating that heat stress occurs between these temperatures in cotton. We chose a leaf temperature of 41°C for the present study because photosynthesis is inhibited by approximately 40% at this temperature, but inhibition is fully reversible (Law and Crafts-Brandner 1999;CraftsBrandner and Law 2000).…”

The role of biomarkers in environmental assessment (4). Terrestrial plants

“…Unfortunately, the synthesis of HSPs is often not related to the heat sensitivity of genotypes (Fender and O'Connell 1989). In addition, HSPs can be induced by other environemntal stress compounds such as arsenite (Lin et al 1984), so that the perspective for HSPs as biomarkers is very limited.…”

Expression of the Heat Shock Response in a Tomato Interspecific Hybrid Is Not Intermediate between the Two Parental Responses

“…While there have been several reports in the literature correlating differences in HSP patterns and differences in heritable thermotolerance (10,23), the genetic proof linking thermotolerance and specific alterations in HSP expression is still lacking. In one case, when the appropriate genetic lines ofcotton were compared, differences in HSP expression observed between thermotolerant and thermosensitive lines were not found to be linked with the thermotolerance phenotype (7).…”

“…While it is apparent that the heat shock response is ubiquitous, variations in the nature of the heat shock response between closely related species have not been well characterized. The heat shock response in plants has been well characterized primarily in two crop plants, soybean (2,8,12) and corn (3, 4), and to a lesser extent in a few other systems (5,7,10,16,17,23). There have been reports on the heat shock response of a cell culture of a wild species of tomato, Lycopersicon peruvianum (19,20,27) sity, including tolerances to abiotic stresses (25).…”