Heat shock protein expression during gametogenesis and embryogenesis

Neuer, A.; Spandorfer, S.D.; Giraldo, Paulo César; Jeremias, Jan; Dieterle, Stefan; Korneeva, Irina; Liu, H.-C.; Rosenwaks, Zev

doi:10.1002/(sici)1098-0997(1999)7:1/2<10::aid-idog3>3.0.co;2-7

Cited by 25 publications

(19 citation statements)

References 40 publications

(16 reference statements)

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“…Defects in this gene have been implicated in Marinesco–Sjögren syndrome, which commonly presents with hypogonadism [48]. Heat-shock proteins are important for proper gametogenesis and embryogenesis, and induction of heat-shock protein-related mRNAs in sperm due to environmental stress could benefit the embryo after fertilization [49]. IFT81, also known as CDV-1, is necessary for the assembly and maintenance of eukaryotic cilia and flagella [50].…”

Section: Discussionmentioning

confidence: 99%

Sperm mRNA Transcripts Are Indicators of Sub-Chronic Low Dose Testicular Injury in the Fischer 344 Rat

et al. 2012

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Current human reproductive risk assessment methods rely on semen and serum hormone analyses, which are not easily comparable to the histopathological endpoints and mating studies used in animal testing. Because of these limitations, there is a need to develop universal evaluations that reliably reflect male reproductive function. We hypothesized that toxicant-induced testicular injury can be detected in sperm using mRNA transcripts as indicators of insult. To test this, we exposed adult male Fischer 344 rats to low doses of model testicular toxicants and classically characterized the testicular injury while simultaneously evaluating sperm mRNA transcripts from the same animals. Overall, this study aimed to: 1) identify sperm transcripts altered after exposure to the model testicular toxicant, 2,5-hexanedione (HD) using microarrays; 2) expand on the HD-induced transcript changes in a comprehensive time course experiment using qRT-PCR arrays; and 3) test these injury indicators after exposure to another model testicular toxicant, carbendazim (CBZ). Microarray analysis of HD-treated adult Fischer 344 rats identified 128 altered sperm mRNA transcripts when compared to control using linear models of microarray analysis (q<0.05). All transcript alterations disappeared after 3 months of post-exposure recovery. In the time course experiment, time-dependent alterations were observed for 12 candidate transcripts selected from the microarray data based upon fold change and biological relevance, and 8 of these transcripts remained significantly altered after the 3-month recovery period (p<0.05). In the last experiment, 8 candidate transcripts changed after exposure to CBZ (p<0.05). The two testicular toxicants produced distinct molecular signatures with only 4 overlapping transcripts between them, each occurring in opposite directions. Overall, these results suggest that sperm mRNA transcripts are indicators of low dose toxicant-induced testicular injury in the rat.

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Section: Discussionmentioning

confidence: 99%

Sperm mRNA Transcripts Are Indicators of Sub-Chronic Low Dose Testicular Injury in the Fischer 344 Rat

et al. 2012

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“…As expression pattern analysis showed ( Figure 3 A,B), HSP60 and HSP10 mRNA transcripts were ubiquitously expressed in all tissues examined and with higher expression in gonads. These results indicated that HSP10 and HSP60 were synthesized constitutively to support the basic metabolism and gonads development [ 8 , 28 ]. Moreover, the expression of HSP10 and HSP60 mRNA transcripts was significantly up-regulated during the onset of migration behavior ( Figure 3 C).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study showed that HSP60 and HSP10 are essential for various cellular processes [ 28 ]. In this study, IHC results revealed that there were higher expression levels of HSP60 and HSP10 in the primary spermatogonia, weak or absent expression was seen in the mature germ cells ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, IHC results revealed that there were higher expression levels of HSP60 and HSP10 in the primary spermatogonia, weak or absent expression was seen in the mature germ cells ( Figure 6 ). These results showed that the protein exists in abundance in germ cells from the beginning of the meiotic phase, which indicated that it is essential for germ proliferation and differentiation [ 18 , 28 , 30 ]. These findings are also in agreement with data from mammal species and suggest that the HSP60 and HSP10 proteins are primarily needed during the initial steps of gametogenesis and migration behavior [ 18 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

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Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

Fang

Zhou

Zhang

et al. 2017

Genes

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Heat shock protein 60 (HSP60) and heat shock protein 10 (HSP10) are important chaperones, which have been proven to have essential roles in mediating the correct folding of nuclear encoded proteins imported to mitochondria. Mitochondria are known as the power house of the cell, with which it produces energy and respires aerobically. In this regard, the obtained HSP60 and HSP10 have typical characteristics of the HSP60/10 family signature. Their mRNA transcripts detected were highest during the developmental phase (in April), while the lowest levels were found in the resting phase (after spawning in late July). Additionally, the strongest immunolabeling positive signals were found in the primary spermatocyte, with lower positive staining in secondary sperm cells, and a weak or absent level in the mature sperm. At the electron microscopic level, immunogold particles were localized in the mitochondrial matrix. Data indicated that HSP10 and HSP60 were inducible and functional in the Coilia nasus testis development and migration process, suggesting their essential roles in this process. The results also indicated that HSP60 may be one indicator of properly working mitochondrial import and refolding in the fish testis. This study also provides an expanded perspective on the role of heat shock protein families in spawning migration biology.

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“…These proteins represent a set of highly conserved molecular chaperones that serve by folding newly synthesized proteins, disassembling unstable proteins, and assisting in the transportation of proteins within the cell [28]. They enable cells to survive adverse environmental conditions, and their absence damages the embryonic development in mice [29,30]. Christensen et al show that homozygosity for the null allele of Hspd1 causes early embryonic lethality, while heterozygosity for the inactivated allele permits embryonic development and postnatal survival [30].…”

Section: Discussionmentioning

confidence: 99%

Proteomics identifies differentially expressed proteins in neonatal murine thymus compared with adults

et al. 2012

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BackgroundThe thymus is an immune organ essential for life and plays a crucial role in the development of T cells. It undergoes a fetal to adult developmental maturation process occurring in mouse during the postnatal months. The molecular modifications underlying these ontogenic changes are essentially unknown. Here we used a differential proteomic-based technique (2D-Difference Gel Electrophoresis) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry to search for key proteins in the postnatal development of the thymus. Eight different BALB/c mice were used in the study: four mice aged of 1 day (neonatal) and four mice aged of 60 days (adult). Protein samples derived from thymus were labeled and run in 2D-PAGE (Two-Dimensional Polyacrylamide Gel Electrophoresis). One whole-thymus tissue from each mouse was run on gels and each gel containing a pooled sample of the eight mice was run in parallel. The pooled sample was set as the internal pool, containing equal amount of each protein extract used in the experiment. Gels were matched and compared with Difference In-gel Analysis software. Differential spots were picked, in-gel digested and peptide mass fingerprints were obtained.ResultsAmong the differentially regulated proteins in neonatal thymus group, 111 proteins were identified by mass spectrometry, of which 95 proteins were up-regulated and 16 proteins were down-regulated. The identified proteins belong to several functional categories, including cell proliferation, cycle and apoptosis, transcription regulation, signal transduction, nucleotide processing, proteolysis and translation, protein folding, metabolism, oxidoreduction, cytoskeleton, immune response, and embryonic development. The major interaction networks comprised of cellular function and maintenance, cellular assembly and organization, and metabolism were also identified by STRING analysis.ConclusionsThe demonstrated molecular changes are relevant for understanding thymus development as well as neonatal immune function, and they provide the diagnostic disease markers. Further studies will be required to describe in detail the role of the identified proteins in thymus maturation and in the specific functions of neonatal thymus.

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Heat shock protein expression during gametogenesis and embryogenesis

Cited by 25 publications

References 40 publications

Sperm mRNA Transcripts Are Indicators of Sub-Chronic Low Dose Testicular Injury in the Fischer 344 Rat

Sperm mRNA Transcripts Are Indicators of Sub-Chronic Low Dose Testicular Injury in the Fischer 344 Rat

Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

Proteomics identifies differentially expressed proteins in neonatal murine thymus compared with adults

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