Heat shock protein expression and autophagy after incomplete thermal ablation and their correlation

Chen, Fen; Bao, Haiwei; Xie, Haiyang; Tian, Guo

doi:10.1080/02656736.2018.1536285

Cited by 26 publications

(23 citation statements)

References 23 publications

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“…These functions include folding of nascent polypeptides, regulation of apoptosis, repair of partially denatured proteins and inhibit protein aggregation, inhibition of heat‐induced apoptosis, and so on, thus protecting cells from stress (Han et al ., ). The heat shock response plays a negative role in the regulation of autophagy (Chen et al ., ). In this study, the expression level of the autophagy‐related genes was upregulated in the Δ VdCmr1 strain as compared with VdLs.17.…”

Section: Discussionmentioning

confidence: 97%

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

Fang

Klosterman

Tian

et al. 2019

Environmental Microbiology

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Summary The fungus Verticillium dahliae causes vascular wilt disease on more than 200 plant species worldwide. This fungus can survive for years in soil as melanized microsclerotia. We found that VdCmr1, a transcription factor, is required for the melanin production and increased survival following UV irradiation in V. dahliae but not for microsclerotia production or virulence. Here, we provided evidence how VdCmr1 protects against high temperature (HT) and UV irradiation in V. dahliae. The results indicate that VdCmr1 mediates entry to the diapause period in V. dahliae in response to HT and contributes to the expression of proteins to minimize protein misfolding and denaturation. VdCmr1 deletion results in the misregulation of DNA repair machinery, suggestive of reduced DNA repair capacity following UV irradiation and in correlation with the low survival rate of UV‐treated VdCmr1 mutants. We discovered a putative VdCmr1‐dependent gene cluster associated with secondary metabolism and stress responses. We also functionally characterized two VdCmr1‐responsive genes participating in HT and UV response. These results shed further light on the roles of VdCmr1 in protection from HT or UV irradiation, and the additional insights into the mechanisms of this protection may be useful to exploit for more effective disease control.

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Section: Discussionmentioning

confidence: 97%

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

Fang

Klosterman

Tian

et al. 2019

Environmental Microbiology

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“…Furthermore, Yang et al (16) describes the inhibition of HSP90 expression and enhancement of apoptosis using Thy-1 membrane glycoprotein (Thy-1)-targeted thermosensitive magnetoliposome-encapsulated 17-aaG for Thy-1 + liver cancer stem cells (lScSs) selected from the Bel-7404 cell line and in a nude mouse model transplanted with Thy-1 + lcScs tumors. To generate the incomplete ablation model, the present study used a laser fiber with a diameter of 300 µm and a transplanted Huh7 tumor mouse to provide a model that can more easily measure molecular changes for subsequent studies (18). our previous study (18) indicated that HSP90 inhibitors may promote apoptosis in the area of incomplete ablation, although an increase in efficiency was not observed.…”

Section: Discussionmentioning

confidence: 97%

“…intraperitoneal anesthesia was administered to the nude mice in the experimental and control groups with approximately 200 µl 4% chloral hydrate at a dose of 400 mg/kg mouse weight. The conditions established in a previous study were applied to build a model of incomplete ablation (18). Mice were fixed on the operating table with local disinfection and the tumor size was observed and measured by high-frequency ultrasound.…”

Section: Methodsmentioning

confidence: 99%

“…The autophagy system serves multiple roles in the degradation of dysfunctional proteins. in the case of incomplete ablation, we first reported the decreased expression of autophagy-associated proteins and a concomitant increase in the expression of HSPs (18). HSP90, as a molecular chaperone, is essential for the proper function of akt as it forms a chaperone-substrate protein complex, and a decrease in HSP90-akt binding results in akt inactivation (19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

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Combination of HSP90 and autophagy inhibitors promotes hepatocellular carcinoma apoptosis following incomplete thermal ablation

et al. 2020

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The present study evaluated the effect of combining inhibitors (17-aaG) of heat shock protein 90 (HSP90) and autophagy (3-Ma) on apoptosis using an incomplete thermal ablation animal model. a total of 28 orthotopic mice with hepatocellular carcinoma were randomly divided into 4 groups to receive different drug interventions. Following palliative laser ablation, changes in autophagy, apoptosis and akt/mTor expression levels were assessed in tumors. compared with the controls, the 17-AAG-treated mice exhibited significantly decreased expression levels of phosphorylated (p)-akt and p-mTor with enhanced autophagy and apoptosis; no marked increases in the expression levels of p-akt and p-mTor were observed in the 3-MA-treated mice, with no significant changes in autophagy; however, apoptosis was enhanced. no significant decreases in p-Akt and p-mTOR or any increase in autophagy were observed in the mice receiving a combination of 17-aaG and 3-Ma, but they did exhibit a marked increase in apoptosis. compared with 17-aaG alone, the combination of 17-aaG and 3-Ma resulted in a marked increase in apoptosis without enhanced autophagy. in the incomplete ablation model, the effects of autophagy and apoptosis are antagonistic. The combined use of 17-AAG and 3-MA can significantly promote apoptosis and is worthy of further study.

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“…Autophagy decreases in incomplete thermal ablation, and its possible inverse correlation with HSP expression has been shown. The HSP90/Akt/mTOR route has been reported, which is associated with signal transmission between autophagy and HSPs (F. Chen, Bao, Xie, Tian, & Jiang, 2019). Dual inhibition of mTOR and HSP90 (Hsp90 inhibitors improve mTOR inhibitor) results in beneficial oncogenic signaling cascades disruption and significantly increases growth inhibition (Lang et al, 2009).…”

Section: Hsp90 As a Therapeutic Target For Hccmentioning

confidence: 99%

The role of HSP90 molecular chaperones in hepatocellular carcinoma

Nouri‐Vaskeh

Alizadeh

Hajiasgharzadeh

et al. 2020

Journal Cellular Physiology

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Misfolded proteins have enhanced formation of toxic oligomers and nonfunctional protein copies lead to recruiting wild‐type protein types. Heat shock protein 90 (HSP90) is a molecular chaperone generated by cells that are involved in many cellular functions through regulation of folding and/or localization of large multi‐protein complexes as well as client proteins. HSP90 can regulate a number of different cellular processes including cell proliferation, motility, angiogenesis, signal transduction, and adaptation to stress. HSP90 makes the mutated oncoproteins able to avoid misfolding and degradation and permits the malignant transformation. As a result, HSP90 is an important factor in several signaling pathways associated with tumorigenicity, therapy resistance, and inhibiting apoptosis. Clinically, the upregulation of HSP90 expression in hepatocellular carcinoma (HCC) is linked with advanced stages and inappropriate survival in cases suffering from this kind of cancer. The present review comprehensively assesses HSP90 functions and its possible usefulness as a potential diagnostic biomarker and therapeutic option for HCC.

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Heat shock protein expression and autophagy after incomplete thermal ablation and their correlation

Cited by 26 publications

References 23 publications

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

Insights into VdCmr1‐mediated protection against high temperature stress and UV irradiation in Verticillium dahliae

Combination of HSP90 and autophagy inhibitors promotes hepatocellular carcinoma apoptosis following incomplete thermal ablation

The role of HSP90 molecular chaperones in hepatocellular carcinoma

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