Heat Shock Protein 90 Stimulates Rat Mesenchymal Stem Cell Migration via PI3K/Akt and ERK1/2 Pathways

Gao, Feng; Hu, Xinyang; Xie, Xing; Liu, Xianbao; Wang, Jianan

doi:10.1007/s12013-014-0228-6

Cited by 37 publications

(23 citation statements)

References 41 publications

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“…We hypothesized that the IGF-1 might affect migratory ability of the mesenchymal stem cell from different origin through different signaling pathways. For example, PI3K-AKT signaling pathway has been demonstrated to hold the potential to regulate the migratory ability of mesenchymal stem cell 25 34 35 . In our study, we also found that the some genes in PI3K-AKT signaling pathway were up-regulated in UC-MSCs-IGF-1 ( Supplementary Figure 3a ), compared with UC-MSCs-vector.…”

Section: Discussionmentioning

confidence: 99%

Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicin-induced acute kidney injury

Liu

Feng

Dong

et al. 2016

Sci Rep

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The therapeutic action of umbilical cord-derived mesenchymal stem cells (UC-MSCs) against acute kidney injury (AKI) has been demonstrated by several groups. However, how to further enhance the renoprotective effect of UC-MSCs and improve the therapy effect, are still unclear. In this study, we mainly investigated whether insulin-like growth factor-1 (IGF-1)-modified UC-MSCs hold an enhanced protective effect on gentamicin-induced AKI in vivo. Our results indicated that the IGF-1 overexpression could enhance the therapeutic action of human UC-MSCs, and the AKI rats treated with IGF-1-overexpressed UC-MSCs (UC-MSCs-IGF-1) showed better recovery of biochemical variables in serum or urine associated with renal function, histological injury and renal apoptosis, compared with AKI rats treated with normal UC-MSCs. RNA microarray analysis indicated that some key genes in the signal pathways associated with anti-oxidation, anti-inflammatory, and cell migratory capacity were up-regulated in UC-MSCs-IGF-1, and the results were further confirmed with qPCR. Furthermore, a series of detection in vitro and in vivo indicated that the UC-MSCs-IGF-1 hold better anti-oxidation, anti-inflammatory, and cell migratory capacity for IGF-1 overexpression. Thus, our study indicated that enhancement of UC-MSCs bioactivities with IGF-1 overexpression could increase the UC-MSCs therapeutic potential and further developed a new therapeutic strategy for the treatment of AKI.

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Section: Discussionmentioning

confidence: 99%

Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicin-induced acute kidney injury

Liu

Feng

Dong

et al. 2016

Sci Rep

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“…It has vital physiological functions such as regulating the activity and function of various proteins, regulating cell proliferation and differentiation, regulating embryonic development, etc. [3,[11][12][13]. Numerous studies have indicated that Hsp has close relations with the genesis and development of various tumors, including non-small-cell lung carcinoma [14], small cell lung carcinoma [4], and breast ductal carcinoma [6].…”

Section: Discussionmentioning

confidence: 99%

The Expression of HSPD1, SCUBE3, CXCL14 and Its Relations with the Prognosis in Osteosarcoma

Liang

Yang

Peng

et al. 2015

Cell Biochem Biophys

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The aim of the study was to explore the expression of three genes, HSPD1, SCUBE3, and CXCL14, in osteosarcoma cells and tissue, as well as their association with the prognosis of patients with osteosarcoma. The expression of HSPD1, SCUBE3, and CXCL14 in osteosarcoma cells was detected by using Western blotting method. siRNA was used to knockdown the expression of the three genes. CCK8 cell proliferation assay was used to observe the effect of siRNA interference on U2OS cell proliferation. The expression of the three genes in osteosarcoma tissue was detected employing immunohistochemical method. Kaplan-Meier survival analysis was used to compare the relations between the expression of the three genes and prognosis. The Western blotting results showed that the expression of Hsp70, SCUBE3 protein, and CXCL14 chemotactic factor in osteosarcoma cells was significantly higher than that in normal osteocytes (p < 0.05). After the three genes were interfered by siRNA, the mRNA and protein expression levels of these genes in osteosarcoma cells were significantly decreased (p < 0.05). The growth rate of U2OS cell after the siRNA interference was significantly lower than that before interference and that in the control group transfected with negative control siRNA (p < 0.05). The result of immunohistochemistry demonstrated that the expression of Hsp70, SCUBE3 protein, and CXCL14 chemotactic factor in osteosarcoma tissues was significantly higher than that in adjacent muscle tissue (p < 0.05). Kaplan-Meier survival analysis indicated that the survival rate of the patients with high expression of those three kinds of genes was obviously lower than that of other patients (p < 0.05). There was no significant difference between the survival rates of patients with high or low expression of two genes (p > 0.05). The expression of HSPD1, SCUBE3, and CXCL14 was all high in osteosarcoma tissues and cells; moreover, the three kinds of genes had close correlations with the prognosis of the patients. Targeted inhibition of these three genes could inhibit the proliferation of the tumor, which may become a new therapeutic target.

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“…Valproic acid, a histone deacetylase inhibitor, enhances MSC migration through the upregulation of both CXCR4 and MMP-2 expression [67]. Further, enhanced gene expression of CXCR4, MMP-2, and MMP-9 by heat shock protein 90 (Hsp90) leads to an increase in the migratory behavior of MSCs [76]. MMP-2 was also found to have a positive role in the transendothelial migration of MSCs, while the increase in culture confluency of MSCs along with MMP-2 siRNA inhibition decreased the transendothelial migration of MSCs and increased the expression of TIMP-3 [77].…”

Section: Mscs Express Mmps and Timps To Regulate Different Processesmentioning

confidence: 99%

Effects of matrix metalloproteinases on the fate of mesenchymal stem cells

2016

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Mesenchymal stem cells (MSCs) have great potential as a source of cells for cell-based therapy because of their ability for self-renewal and differentiation into functional cells. Moreover, matrix metalloproteinases (MMPs) have a critical role in the differentiation of MSCs into different lineages. MSCs also interact with exogenous MMPs at their surface, and regulate the pericellular localization of MMP activities. The fate of MSCs is regulated by specific MMPs associated with a key cell lineage. Recent reports suggest the integration of MMPs in the differentiation, angiogenesis, proliferation, and migration of MSCs. These interactions are not fully understood and warrant further investigation, especially for their application as therapeutic tools to treat different diseases. Therefore, overexpression of a single MMP or tissue-specific inhibitor of metalloproteinase in MSCs may promote transdifferentiation into a specific cell lineage, which can be used for the treatment of some diseases. In this review, we critically discuss the identification of various MMPs and the signaling pathways that affect the differentiation, migration, angiogenesis, and proliferation of MSCs.

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Heat Shock Protein 90 Stimulates Rat Mesenchymal Stem Cell Migration via PI3K/Akt and ERK1/2 Pathways

Cited by 37 publications

References 41 publications

Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicin-induced acute kidney injury

Enhanced renoprotective effect of IGF-1 modified human umbilical cord-derived mesenchymal stem cells on gentamicin-induced acute kidney injury

The Expression of HSPD1, SCUBE3, CXCL14 and Its Relations with the Prognosis in Osteosarcoma

Effects of matrix metalloproteinases on the fate of mesenchymal stem cells

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