Heat Shock Protein 75 (TRAP1) facilitate the proliferation of the Bombyx mori nucleopolyhedrovirus

Wang, Xiong; Zhang, Yinong; Fei, Shigang; Awais, Mian Muhammad; Zheng, Hao; Feng, Mei; Sun, Jingchen

doi:10.1016/j.ijbiomac.2021.01.213

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…The other viral particle is budded virions, which mainly spread between cells and lead to secondary infection of internal tissues (Rahman & Gopinathan, 2004;Clem & Passarelli, 2013). In recent years, several endogenous silkworm proteins were found to be related to the replication of BmNPV, including Ser/Thr protein phosphatase 2A (Hu et al, 2020), heat shock protein 75 (Wang et al, 2021), cholesterol-25-hydroxylase (Wu et al, 2023), and sirtuin 5 (Zhang et al, 2022). Fatty acids play crucial roles in living organisms by contributing to cell membrane composition, intracellular signal transduction, and energy metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Bombyx mori nucleopolyhedrovirus induces BmFABP1 downregulation to promote viral proliferation

Wang

Ren

et al. 2023

Insect Science

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Fatty acid binding proteins (FABPs) play an important role as endogenous cytoprotectants. However, studies on FABPs in invertebrates are scarce. Previously, we discovered Bombyx mori fatty acid binding protein 1 (BmFABP1) through co‐immunoprecipitation. Here, we cloned and identified BmFABP1 from BmN cells. The results of immunofluorescence indicated that BmFABP1 was localized in the cytoplasm. The tissue expression profile of silkworms showed that BmFABP1 was expressed in all tissues except hemocytes. The expression level of BmFABP1 gradually decreases in BmN cells and B. mori larvae after infection with B. mori nucleopolyhedrovirus (BmNPV). Upregulation of BmFABP1 expression through overexpression or WY14643 treatment significantly inhibited the replication of BmNPV, while downregulation of BmFABP1 expression by RNA interference promoted the replication of BmNPV. The same results were obtained in experiments on silkworm larvae. These results suggest that BmNPV induces BmFABP1 downregulation to promote its proliferation and that BmFABP1 has a potential anti‐BmNPV role. This is the first report on the antiviral effect of BmFABP1 in silkworms and provides new insights into the study of the FABP protein family. Also, it is important to study BmNPV resistance in silkworms to breed transgenic silkworms with BmNPV resistance.

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Section: Introductionmentioning

confidence: 99%

“…, 2020), heat shock protein 75 (Wang et al. , 2021), cholesterol‐25‐hydroxylase (Wu et al. , 2023), and sirtuin 5 (Zhang et al.…”

Section: Introductionmentioning

confidence: 99%

Bombyx mori nucleopolyhedrovirus induces BmFABP1 downregulation to promote viral proliferation

Wang

Ren

et al. 2023

Insect Science

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“…However, Bombyx mori nucleopolyhedrovirus (BmNPV) is a major pathogen in silkworm breeding that causes serious economic loss to the sericulture industry worldwide [ 17 ]. BmNPV is a typical baculovirus belonging to Group I, with glycoprotein 64 as the main envelope protein [ 18 ]. Improving the resistance of silkworms to BmNPV has important practical significance for sericulture.…”

Section: Introductionmentioning

confidence: 99%

A Protein Asteroid with PIN Domain in Silkworm Bombyx mori Is Involved in Anti-BmNPV Infection

Xia

Jiang

et al. 2023

Insects

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Nuclease is a type of protein that degrades nucleic acids, which plays an important role in biological processes, including RNA interference efficiency and antiviral immunity. However, no evidence of a link between nuclease and Bombyx mori nucleopolyhedrovirus (BmNPV) infection in silkworm B. mori has been found. In this study, a protein asteroid (BmAst) containing the PIN domain and XPG domain was identified in silkworm B. mori. BmAst gene was highest expressed in hemocytes and fat body of the 5th instar larvae, and high expression in the pupa stage. The transcriptional levels of the BmAst gene in 5th instar larvae were significantly induced by BmNPV or dsRNA. After knocking down BmAst gene expression by specific dsRNA, the proliferation of BmNPV in B. mori was increased significantly, whereas the survival rate of larvae was significantly lower when compared with the control. Our findings indicate that BmAst is involved in silkworm resistance to BmNPV infection.

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“…The invasion and replication mechanisms of BmNPV remain largely unknown, and in particular, the investigation of its receptor protein is warranted. Recently, some proteins were shown to be associated with the invasion and replication of BmNPV, including TRAP1 protein, a member of the heat shock protein 90 family [ 24 ], E3 ubiquitin-protein ligase SINA-like 10 (SINAL10) [ 25 ], B. mori nuclear hormone receptor 96 (BmNHR96) [ 26 ], and B. mori receptor expression-enhancing protein (BmREEPa) [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

Wang

Ren

et al. 2022

Insects

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Virus–host interactions are critical for virus replication, virulence, and pathogenicity. The Bombyx mori nucleopolyhedrovirus (BmNPV) is a typical model baculovirus, representing one of the most common and harmful pathogens in sericulture. Herein, we used co-immunoprecipitation to identify candidate proteins with potential interactions with BmNPV. First, a recombinant BV virus particle rBmBV-egfp-p64-3×flag-gp64sp was constructed using a MultiBac baculovirus multigene expression system. Co-immunoprecipitation experiments were then performed with the recombinant BV virus infected with BmN cells and Dazao silkworms. LC-MS/MS analysis revealed a total of 845 and 1368 candidate proteins were obtained from BmN cells and silkworm samples, respectively. Bioinformatics analysis (Gene Ontology, KEGG Pathway) was conducted for selection of proteins with significant enrichment for further confirmation of the effects on BmNPV replication. Overall, the results showed that SEC61 and PIC promoted the replication of BmNPV, while FABP1 inhibited the replication of BmNPV. In summary, this study reveals the potential proteins involved in BmNPV invasion and proliferation in the host and provides a platform for identifying the potential receptor proteins of BmNPV.

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Heat Shock Protein 75 (TRAP1) facilitate the proliferation of the Bombyx mori nucleopolyhedrovirus

Cited by 8 publications

References 26 publications

Bombyx mori nucleopolyhedrovirus induces BmFABP1 downregulation to promote viral proliferation

Bombyx mori nucleopolyhedrovirus induces BmFABP1 downregulation to promote viral proliferation

A Protein Asteroid with PIN Domain in Silkworm Bombyx mori Is Involved in Anti-BmNPV Infection

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

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