Heat shock protein 70 kDa (HSP70) increase in sea bass (Dicentrarchus labrax, L 1758) thymus after vaccination against Listonella anguillarum

Abstract

Heat shock proteins 70 kDa (HSP70) and apoptosis were investigated in thymus of sea bass juveniles (Dicentrarchus labrax) subsequently to a vaccination against Listonella (syn. Vibrio) anguillarum. HSP70 expression was measured by immunohistochemistry and immunoenzymatic methods, resulting in increase in HSP70 after bath immunization and persistent in fish exposed to an intraperitoneal (i.p.) booster. The HSP70 increase in thymus was suggested as induction in lymphocytic cells, to be related to immune system stimulation after vaccination. However, a thymic recruitment of lymphocyte subpopulations, characterized by higher expression of HSP70, was also hypothesized after vaccination. No apparent relationships were found between HSP70 and apoptosis. In fact, the vaccination did not modulate the apoptosis response, as measured by TUNEL assay and by immunohistochemistry for active caspase-3 expression. The lack of apoptosis effects could be ascribed to the use of inactivated bacteria that appeared not able to interfere with programmed cell death mechanisms. This manuscript aims to contribute to the knowledge of some biochemical features underlying the immunization, with a particular emphasis on the modulation of HSP70. However, further parameters involved in innate/adaptative immunity and apoptosis pathways have to be taken into account to well establish the functional role of HSP70 in fish vaccination.

Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)

“…plays an important role in the apoptotic process (Zhang et al 2008). Caspase-3 is the marker protein of apoptosis participating in degradation of nuclear and cytoplasmic proteins during this process (Kondera and Witeska 2013;Mosca et al 2013). Lang et al (2003Lang et al ( , 2006 reported that apoptosis is Ca 2?…”

Transcriptomic analyses of juvenile Striped Bass (Morone saxatilis) exposed to chronic and acute temperature change

“…The dotted studied in the related European Sea Bass (Dicentrarchus labrax), though much of this research used a targeted gene approach and focused on aquaculture practices. In D. labrax, significant induction of hsp70 has been found following changes in diet [29,30], handling stress [31], vaccination [32] and during larval development [33]. Cheng, Xia [34] used RNA-seq to assess transcriptomic changes in D. labrax after infection by a common pathogen, but found no induction of any HSP-related genes, while the pufferfish (Takifugu rubripes) also assessed in this study did show HSP induction.…”

“…In D . labrax , significant induction of hsp70 has been found following changes in diet [ 29 , 30 ], handling stress [ 31 ], vaccination [ 32 ] and during larval development [ 33 ]. Cheng, Xia [ 34 ] used RNA-seq to assess transcriptomic changes in D .…”

“…In addition to the commonly studied hsp70 and hsp90, we also found upregulation of multiple genes related to hsp40 in response to increased temperature. While many HSP-related research in fish biology focuses on hsp70 and hsp90 [11,32,[35][36][37], it is not surprising to find induction of hsp40 as well. hsp40 is believed to work co-operatively with hsp70's in protein folding, transport and degradation [38].…”

Micro RNAs are involved in activation of epicardium during zebrafish heart regeneration

“…However, miR-1 is a direct repressor of HSP70 and its upregulation was demonstrated during the regeneration of zebrafish heart 5 . Some years ago, we optimized the immunohistochemistry for HSP70 antibody 42 , and recently, also for the zebrafish heart. In our finding, already after 24–48 h, a strong positivity to HSP70 in the RC has been evident.…”