Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis

Galović, Ružica; Flegar-Meštrić, Zlata; Vidjak, Vinko; Matokanović, Mirela; Barišić, Karmela

doi:10.1016/j.clinbiochem.2015.10.006

Cited by 19 publications

(9 citation statements)

References 26 publications

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“…Mast cells, dendrite cells, and even tumor cells had also been observed to be a source of circulating HSP70s (Laulagnier et al 2004;Mambula and Calderwood 2006). Our result of elevated plasma HSP70 is consistent with the data from Krepuska (Krepuska et al 2011) and Wright (Wright et al 2000), which showed an increased level of serum HSP70 (HSPA1A) in atherosclerosis patients, but is in contrast to other reports showing a lower concentration of circulating HSP70 (HSPA1A) in atherosclerotic subjects (Dulin et al 2010;Galovic et al 2016). The reasons for this conflict have not been fully understood, but perhaps the differences in the models and species may contribute to explain the contrasting findings.…”

Section: Discussioncontrasting

confidence: 57%

Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats

Xie¹,

Zhan²,

Yan³

et al. 2016

Cell Stress and Chaperones

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Although accumulating evidence indicates that heat shock protein 70 (HSP70) could be secreted into plasma and its levels have been found to have an ambiguous association with atherosclerosis, our knowledge for the exact role of circulating HSP70 in the development of atherosclerosis is still limited. In the present study, we report an adhesion-promoting effect of exogenous HSP70 and evaluate the potential involvement of elevated circulating HSP70 in the development of atherosclerosis. Time-dependent elevation of plasma HSP70 was found in diet-induced atherosclerotic rats, whose effect was investigated through further in vitro experiments. In rat aortic endothelial cell (RAEC) cultures, exogenous HSP70 incubation neither produced cell injuries by itself nor had protective effects on cell injuries caused by Ox-LDL or homocysteine. However, exogenous HSP70 administration could lead to a higher adhesion rate between rat peripheral blood monocytes (PBMCs) and RAECs. This adhesion-promoting effect appeared only when PBMCs, rather than RAECs, were pretreated with HSP70 incubation. PBMCs in an HSP70 environment released more IL-6 to supernatant, which subsequently up-regulated the expression of ICAM-1 in RAECs. These results indicate that the diet-induced elevation of circulating HSP70 could trigger cell adhesion with the help of IL-6 as a mediator, which provides a novel possible mechanism for understanding the role of circulating HSP70 in the pathogenesis of atherosclerosis.

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Section: Discussioncontrasting

confidence: 57%

Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats

Xie¹,

Zhan²,

Yan³

et al. 2016

Cell Stress and Chaperones

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“…Circulating anti-oxLDL antibodies and oxLDL may be optimal biomarkers for cardiovascular disease risk, however, their true significance remains uncertain [29]. Although increased levels of autoantibody against heat shock protein (Hsp) family are reported in patients with arteriosclerotic diseases [30,31], their ability to predict the occurrence of arteriosclerotic diseases remains unclear. Sun et al [32] reported that higher circulating ESM-1, previously named endothelial cell-specific molecule-1, correlated with the presence of CAD in patients with OSA.…”

Section: Discussionmentioning

confidence: 99%

Circulating Anti-Sorting Nexins 16 Antibodies as an Emerging Biomarker of Coronary Artery Disease in Patients with Obstructive Sleep Apnea

et al. 2020

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Biomarkers are not available for monitoring the onset and progression of coronary artery disease (CAD) in patients with obstructive sleep apnea (OSA), a major risk factor for arteriosclerotic cardiovascular diseases. This study aimed to test for correlation between circulating anti-Sorting Nexins 16 antibody (SNX16-Ab) levels, CAD history and clinical parameters of patients with OSA. Sixty-four healthy donors, 82 adults with OSA, and 96 with acute coronary syndrome (ACS) were studied. Serum samples were collected at diagnostic polysomnography in the OSA group or at the disease onset in the ACS group. Serum SNX16-Ab levels were measured by amplified luminescence proximity homogeneous assay (AlphaLISA), and correlation between SNX16-Ab levels and clinical parameters was analyzed. SNX16-Ab levels and apnea-hypopnea index (AHI) were weakly correlated. Additionally, logistic regression analyses of OSA group identified that elevated SNX16-Ab level associated with the history of CAD. Circulating SNX16-Ab could increase during CAD pathogenesis in patients with OSA. Further prospective studies are required to prove the predictive potential of SNX16-Ab level in CAD onset of patients with OSA.

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“…Considering these reports in different tissues with our findings that the yaks received different stimuli under normal conditions, we speculated that the expression levels of HSP70/90 are regulated to protect animals. Previous researches found that different stimuli could induce significantly different HSPs expression levels in the bronchioles and respiratory bronchiole epithelial cells [37]. HSP70/90 are shown to play regulatory role in the adaptation to the high temperature and low salinity in marine environments [38].…”

Section: Discussionmentioning

confidence: 99%

Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue

Liu

et al. 2017

PLoS ONE

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The aim of this study is to investigate the expression and localization of HSP70/90 in different tissues and explore the regulation effects of HSP70/90 at lactation period of female yaks. HSP90 mRNA was cloned from the heart samples of female yaks, Quantitative real-time (qRT-PCR), Western blotting (WB), immunohistochemistry and immunofluorescence assays were utilized to analyze the expressions of HSP70/90 mRNA and protein in different tissues. Sequence analysis showed that HSP90 is a conserved molecular chaperone of female yaks. The qRT-PCR, WB results showed that the expressions of HSP70/90 mRNA and protein were significantly different in different tissues, and 3-fold higher expression during the lactation period than the non-lactation period of breast tissue (P < 0.01). Immunohistochemistry and immunofluorescence assays results showed that HSP70/90 were located in the cardiac muscle cells, cerebellar medulla, theca cells lining at the reproductive system, and the mammary epithelia of the breasts. In addition, the expression level of HSP70 was higher than those of HSP90 in all examined tissues. Therefore, our results strongly suggest that the expression and localization of HSP70/90 could provide significant evidence to further research in tissue specific expression, and lactation function of female yaks.

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Heat shock protein 70 and antibodies to heat shock protein 60 are associated with cerebrovascular atherosclerosis

Cited by 19 publications

References 26 publications

Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats

Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats

Circulating Anti-Sorting Nexins 16 Antibodies as an Emerging Biomarker of Coronary Artery Disease in Patients with Obstructive Sleep Apnea

Cloning of HSP90, expression and localization of HSP70/90 in different tissues including lactating/non-lactating yak (Bos grunniens) breast tissue

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