Heat shock protein 60 expression and localisation in different tissues and testis development of male cattle (cattle-yak and yak)

Zou, Shengnan; Liu, P; Yu, Sijiu; Cui, Yan; He, Junlin; Afedo, Seth Yaw; Zhang, H; Niayale, Robert; Zhao, Kai

doi:10.5603/fm.a2020.0126

Cited by 10 publications

(2 citation statements)

References 38 publications

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“…The free-range mode may lead to the insufficient nutritional intake of yaks, and there is insufficient nutrient to use during spermatogenesis, resulting in poor semen quality. Conversely, yaks raised in housefeeding mode are fed a basal diet with balanced nutrition, and their sperm motility can exceed 70% (61), which is consistent with our study. Since the artificial insemination technology of yaks is not mature enough compared to pigs, changing the feeding mode is an effective way to improve the semen quality of yaks.…”

Section: Discussionsupporting

confidence: 90%

House feeding pattern increased male yak fertility by improving gut microbiota and serum metabolites

Zhu

Li²,

Lousang-zhaxi³

et al. 2022

Front. Vet. Sci.

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Yaks usually live in an extremely harsh natural environment resulting in low reproductive performance, so the production of yak cannot meet local demand in China. In order to solve this problem, the experiment aims to explore the effect of different feeding modes on the semen quality of male yaks, so as to provide a theoretical basis for improving the yield of yaks in Tibet. We used the combined analysis of metabolomics and microbial sequencing to explore the underlying mechanisms that affect the differences in semen quality between the house feeding (HF) system and the free range (FR). The results showed that the sperm motility (P < 0.001) and sperm concentration (P < 0.05) in the HF group were significantly higher than the FR group, and the abnormal sperm rate (P < 0.01) in HF was significantly lower compared to FR. House feeding modes increased some beneficial materials in blood and testis especially some antioxidants, unsaturated fatty acids, and amino acids. House feeding group increased some gut microbiota at genus level namely Rikenellaceae, Bacteroides, Prevotellaceae_UCG-004, Bacteroidales_RF16, and Alloprevotella, DgA-11. It was interesting that blood metabolites, testicular metabolites, and fecal microbiota were well-correlated with sperm parameters. Meanwhile, the blood metabolites and testicular metabolites were well-correlated with microbes. The result indicated that the HF model was beneficial for yak semen quality by improving the gut microbiota and blood metabolism to increase yak fertility.

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Section: Discussionsupporting

confidence: 90%

House feeding pattern increased male yak fertility by improving gut microbiota and serum metabolites

Zhu

Li²,

Lousang-zhaxi³

et al. 2022

Front. Vet. Sci.

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show abstract

“…The testis is a vital male reproductive organ composed primarily of spermatogenic tubules and testis mesenchyme [1]. The seminiferous tubules consist of a boundary membrane and a seminiferous epithelium.…”

Section: Introductionmentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals Atlas of Yak Testis Cells

Wang

Pei

Xiong

et al. 2023

IJMS

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Spermatogenesis is a complex process that involves proliferation and differentiation of diploid male germ cells into haploid flagellated sperm and requires intricate interactions between testicular somatic cells and germ cells. The cellular heterogeneity of this process presents a challenge in analyzing the different cell types at various developmental stages. Single-cell RNA sequencing (scRNA-seq) provides a useful tool for exploring cellular heterogeneity. In this study, we performed a comprehensive and unbiased single-cell transcriptomic study of spermatogenesis in sexually mature 4-year-old yak using 10× Genomics scRNA-seq. Our scRNA-seq analysis identified six somatic cell types and various germ cells, including spermatogonial stem cells, spermatogonia, early-spermatocytes, late-spermatocytes, and spermatids in yak testis. Pseudo-timing analysis showed that Leydig and myoid cells originated from common progenitor cells in yaks. Moreover, functional enrichment analysis demonstrated that the top expressed genes in yak testicular somatic cells were significantly enriched in the cAMP signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, and ECM receptor interactions. Throughout the spermatogenesis process, genes related to spermatogenesis, cell differentiation, DNA binding, and ATP binding were expressed. Using immunohistochemical techniques, we identified candidate marker genes for spermatogonial stem cells and Sertoli cells. Our research provides new insights into yak spermatogenesis and the development of various types of cells in the testis, and presents more reliable marker proteins for in vitro culture and identification of yak spermatogonial stem cells in the later stage.

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Expression of tumour transcription factor GLI1 in canine mammary tumours tissue

Liu

Yang

et al. 2022

Veterinary Medicine & Sci

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Background: Mammary tumor is one of the most common diseases of canine in pet clinics.Objectives: This study investigates the distribution and expression of the tumor transcription factor GLI1 and the downstream proteins, Bmi1 and Sox2, in canine mammary tumors and paracancerous tissues.Methods: Cancerous and paracancerous normal mammary tissues were detected using western blotting (WB), and immunohistochemistry. Results:The results showed that the histopathology of different types in mammary tumors by microscopic observation. GLI1/Bmi1/Sox2 expression was significantly higher in canine mammary invasive carcinoma than in ductal carcinoma and adjacent normal mammary tissues (p < 0.01). The expression of GLI1 in invasive carcinoma tissues was significantly higher than Bmi1 and Sox2, while Sox2 expression in ductal carcinoma tissues was significantly higher than GLI1 and Bmi1 (p < 0.01). GLI1/Bmi1/Sox2 all showed positive reactions in both mammary tumor and adjacent normal mammary tissues with immunohistochemistry. GLI1 and Sox2 showed strong positive staining in the cytoplasm of invasive mammary carcinoma and ductal carcinoma cells, and weak positive staining in the nuclei. The positive Bmi1 reaction was mainly concentrated in the cytoplasm of invasive carcinoma and ductal carcinoma cells, while the positive reaction on the cell membrane was weak. Conclusions:We speculate that GLI1 and related proteins play an important role in regulating the proliferation and differentiation of tumors. Therefore, it provides important reference for the pathogenesis and pathogenicity of canine mammary tumor.

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Heat shock protein 60 expression and localisation in different tissues and testis development of male cattle (cattle-yak and yak)

Cited by 10 publications

References 38 publications

House feeding pattern increased male yak fertility by improving gut microbiota and serum metabolites

House feeding pattern increased male yak fertility by improving gut microbiota and serum metabolites

Single-Cell RNA Sequencing Reveals Atlas of Yak Testis Cells

Expression of tumour transcription factor GLI1 in canine mammary tumours tissue

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