Heat shock‐mediated APX gene expression and protection against chilling injury in rice seedlings

Satō, Yutaka; Murakami, Tetuhide H.; Funatsuki, Hideyuki; Matsuba, Shuichi; Saruyama, Haruo; Tanida, Masatoshi

doi:10.1093/jexbot/52.354.145

Cited by 150 publications

(116 citation statements)

References 34 publications

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“…Among the many processes that have been studied and published, the regulating role of kinases dependent on calcium and activated by cold temperature exposition (Saijo et al, 2000;Martín & Busconi, 2001); the involvement of abscisic acid as a signalling molecule (Lee et al, 1995); and the levels of activation of the enzymes associated to oxidative stress such as ascorbate peroxidase (Sato et al, 2001), catalase and glutathione reductase (Kang & Saltveit, 2002) may be cited. Despite the lack of knowledge regarding the germination period of rice, the existence of different physiological mechanisms involved with cold tolerance at the vegetative period is an indication that along the germination phases, different processes may also be affected, implying in different tolerance mechanisms.…”

Section: Resultsmentioning

confidence: 99%

Cold tolerance at the germination stage of rice: methods of evaluation and characterization of genotypes

Cruz¹,

Milach

2004

Sci. agric. (Piracicaba, Braz.)

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Rice cold tolerance at the germination stage is important in Rio Grande do Sul (RS) where temperatures below 15°C prevent or reduce germination and plant establishment in early sowings. The present study aimed at identifying an adequate method for cold tolerance evaluation of the rice germination stage and at verifying the variability among 24 rice genotypes of different origins. Cold tolerance was evaluated in experiment I, germination under two conditions: 13°C for 28 days and 28°C for seven days, and in experiment II, germination under 28°C for 72 hours, 13°C for 96 hours and again 28°C for 72 hours. In experiment I measured characteristics were germination index, percentage of seeds with coleoptile length superior to 5 mm and percentage of reduction in coleoptile length due to cold. In experiment II the measured characteristic was coleoptile regrowth after the cold period. Cold tolerance varied among genotypes studied in both experiments, but only the percentage of reduction in coleoptile length and coleoptile regrowth allowed a better distinction between the tolerant checks and the susceptible one. In general, genotypes belonging to the Japonica subspecies presented higher cold tolerance than Indica, but there was variability within subspecies. The most adequate method of evaluation of cold tolerance is through percentage of reduction in coleoptile length and coleoptile regrowth. Among Japonica genotypes, Quilla 64117 and Diamante presented the highest cold tolerance, and among Indica, cultivars BR-IRGA 410 and IRGA 416 were the most cold tolerant at the germination stage. Key words: Oryza sativa L., low temperature, variability, Indica and Japonica subspecies TOLERÂNCIA AO FRIO NO ESTÁDIO DE GERMINAÇÃO EM ARROZ: MÉTODOS DE AVALIAÇÃO E CARACTERIZAÇÃO DE GENÓTIPOSRESUMO: A tolerância ao frio em arroz no estádio de germinação é importante no Rio Grande do Sul (RS) onde temperaturas abaixo de 15°C impedem ou reduzem a germinação e o estabelecimento das plantas em semeaduras precoces. O presente trabalho teve por objetivos identificar uma metodologia adequada para avaliação da tolerância ao frio na germinação em arroz e verificar a variabilidade existente entre 24 genótipos de arroz de diferentes origens. A tolerância ao frio foi avaliada em dois experimentos: experimento I, com germinação sob duas condições: 13°C por 28 dias e 28°C por sete dias, e experimento II, com germinação a 28°C por 72 horas, 13°C por 96 horas e novamente a 28°C por 72 horas. No experimento I as características medidas foram o índice de germinação, porcentagem de sementes com coleóptilo superior a 5 mm e porcentagem de redução no comprimento do coleóptilo devido ao frio. No experimento II a característica medida foi o recrescimento do coleóptilo após o período de frio. Houve variabilidade para tolerância ao frio entre os genótipos estudados em ambos os experimentos, mas apenas a porcentagem de redução no comprimento do coleóptilo e o recrescimento do mesmo permitiram melhor distinção entre as testemunhas tolerantes e a sensíve...

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Section: Resultsmentioning

confidence: 99%

Cold tolerance at the germination stage of rice: methods of evaluation and characterization of genotypes

Cruz¹,

Milach

2004

Sci. agric. (Piracicaba, Braz.)

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“…Consistent with our results, SOD also has been shown to be down-regulated at the protein and transcript levels by several oxidative stresses [48] or heat [56], respectively. In addition, Sato et al [57] reported that SOD activity was not altered in rice seedlings exposed to heat (427C). These results suggest that SOD responds to thermal stress in a complex manner or that isoforms could be differentially regulated.…”

Section: Redox Homeostasismentioning

confidence: 99%

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

et al. 2007

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The present study investigated rice leaf proteome in response to heat stress. Rice seedlings were subjected to a temperature of 427C and samples were collected 12 and 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress frequently was generated in rice leaves exposed to high temperature. 2-DE, coupled with MS, was used to investigate and identify heat-responsive proteins in rice leaves. In order to identify the low-abundant proteins in leaves, samples were prefractionated by 15% PEG. The PEG supernatant and the pellet fraction samples were separated by 2-DE, and visualized by silver or CBB staining. Approximately 1000 protein spots were reproducibly detected on each gel, wherein 73 protein spots were differentially expressed at least at one time point. Of these differentially expressed proteins, a total of 34 and 39 protein spots were found in the PEG supernatant and pellet fractions, respectively. Using MALDI-TOF MS, a total of 48 proteins were identified. These proteins were categorized into classes related to heat shock proteins, energy and metabolism, redox homeostasis, and regulatory proteins. The results of the present study show that a group of low molecular small heat shock proteins (sHSPs) were newly induced by heat stress. Among these sHSPs, a low molecular weight mitochondrial (Mt) sHSP was validated further by Western blot analysis. Furthermore, four differentially accumulated proteins that correspond to antioxidant enzymes were analyzed at the mRNA level, which confirmed the differential gene expression levels, and revealed that transcription levels were not completely concomitant with translation. The identification of some novel proteins in the heat stress response provides new insights that can lead to a better understanding of the molecular basis of heat-sensitivity in plants.

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“…This enzyme was induced by cold in fruitfly (Drosophila melanogaster; Duncker et al, 1995). Because chilling induces oxidative stress (Sato et al, 2001), it is possible that the sugarcane XDH is induced in response to oxidative stress generated by cold exposure. Another protein related to antioxidant metabolism is copper/zinc SOD (Cu/ZnSOD).…”

Section: Cold-responsive Genes In Sugarcanementioning

confidence: 99%

RNA Expression Profiles and Data Mining of Sugarcane Response to Low Temperature

et al. 2003

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Tropical and subtropical plants are generally sensitive to cold and can show appreciable variation in their response to cold stress when exposed to low positive temperatures. Using nylon filter arrays, we analyzed the expression profile of 1,536 expressed sequence tags (ESTs) of sugarcane (Saccharum sp. cv SP80-3280) exposed to cold for 3 to 48 h. Thirty-four cold-inducible ESTs were identified, of which 20 were novel cold-responsive genes that had not previously been reported as being cold inducible, including cellulose synthase, ABI3-interacting protein 2, a negative transcription regulator, phosphate transporter, and others, as well as several unknown genes. In addition, 25 ESTs were identified as being down-regulated during cold exposure. Using a database of cold-regulated proteins reported for other plants, we searched for homologs in the sugarcane EST project database (SUCEST), which contains 263,000 ESTs. Thirty-three homologous putative coldregulated proteins were identified in the SUCEST database. On the basis of the expression profiles of the cold-inducible genes and the data-mining results, we propose a molecular model for the sugarcane response to low temperature.

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Heat shock‐mediated APX gene expression and protection against chilling injury in rice seedlings

Cited by 150 publications

References 34 publications

Cold tolerance at the germination stage of rice: methods of evaluation and characterization of genotypes

Cold tolerance at the germination stage of rice: methods of evaluation and characterization of genotypes

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

RNA Expression Profiles and Data Mining of Sugarcane Response to Low Temperature

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