Heat-shock-induced oxidative stress and antioxidant response in Aspergillus niger 26

Abstract

To extend the knowledge about the relationship between heat shock and oxidative stress in lower eukaryotes, the filamentous fungus Aspergillus niger 26 was chosen as a model system. Here, the response of A. niger cells to heat shock is reported. The temperature treatment significantly increased the levels of reactive oxygen species, superoxide anions (O2), and hydrogen peroxide and the rate of cyanide-resistant respiration as a marker of oxidative stress. Enhanced reactive oxygen species generation coincided with an increase in the content of oxidative damaged protein and in the accumulation of the storage carbohydrates trehalose and glycogen. Thermal survival of the A. niger cells corresponded to a significant increase in the levels of the antioxidant enzymes superoxide dismutase and catalase for all variants. These observations suggest that heat and oxidative stress have a common cellular effect.

Transcriptomic Analysis of Temperature Responses of Aspergillus kawachii during Barley Koji Production

“…For A. kawachii, cultivation at 40°C appears to induce a stress response because several heat shock proteins and chaperones are significantly upregulated at this temperature, based on the results of GO analysis (Table 4). This finding is in agreement with a previous report that found that heat shock stress induces oxidative stress and an antioxidant response in A. niger, resulting in increased accumulation of the storage carbohydrates trehalose and glycogen (38). It was suggested that trehalose mobilization is required to facilitate cell recovery by A. niger after heatinduced damage (39).…”

Hyperthermia Sensitizes Rhizopus oryzae to Posaconazole and Itraconazole Action through Apoptosis

“…Apoptosis has been observed in a number of filamentous fungi, such as Aspergillus nidulans, Aspergillus fumigatus, Neurospora crassa, and Fusarium oxysporum (12)(13)(14)(15). Various physiological stresses, ionizing radiation, and hyperthermia have been reported to trigger apoptosis in fungi and cancer cells (14)(15)(16).…”

“…Various physiological stresses, ionizing radiation, and hyperthermia have been reported to trigger apoptosis in fungi and cancer cells (14)(15)(16). However, the precise mechanism of hyperthermia-induced apoptosis in mammalian cells is not clear.…”

Growth-Phase-Related Changes in Reactive Oxygen Species Generation as a Cold Stress Response in AntarcticPenicilliumStrains

“…Similar changes have been reported for other fungi and yeasts: Fusarium decemcellulare (35), P. chrysogenum (42), Saccharomyces cerevisiae (28), Candida albicans (32) etc. our previous studies also demonstrated enhanced RoS production in Humicola lutea and Aspergillus niger cells, threated by cu-and heat stress, respectively (1,30).…”

“…A variety of exogenous stimuli, such as radiation, pathogen infections, and exposure to xenobiotics can also cause in vivo RoS production (29). Additionally, several specific types of environmental stress can lead to the production of RoS, including heat stress (1), herbicide/insecticide contamination (2,3), ultraviolet light exposure (4,21), etc.…”