Heat Shock-induced Necrosis and Apoptosis in Osteoblasts

Li, Song; Chien, Shu; Brånemark, Per‐Ingvar

doi:10.2106/00004623-200004000-00040

Cited by 21 publications

(31 citation statements)

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“…Because the ambient and cement temperature is a predominant parameter for the curing of the cement, cement hardening behaviour was investigated at different ambient and cement temperatures. Ambient temperature range applied for the testing was defined in order to cover the range [18][19][20][21][22][23][24][25][26] • C given in the DIN 1946-4, 1999-03 standard.…”

Section: Methodsmentioning

confidence: 99%

“…The working time, beginning with reaching an appropriate viscosity, ranges from 4.5 to around 12 minutes. Up till now no approved cement shows a low waiting time and a long working time above 12 minutes at an ambient temperature range from [19][20][21][22][23][24][25][26] • C, without requiring high force injection systems. In order to address the reduction in waiting time, sufficient working time and the compatibility with syringe systems, the development of a new PMMA vertebroplasty cement was undertaken.…”

Section: Introductionmentioning

confidence: 99%

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Rheological and Curing Behavior of a Newly Developed, Medium Viscous Acrylic Bone Cement

Deußer¹,

Sattig²,

Böger³

2011

ISRN Materials Science

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Percutaneous vertebroplasty, comprising an injection of polymethylmethacrylate (PMMA) bone cement into vertebral bodies, is a practical procedure for stabilization of osteoporotic compression fractures and other weakening lesions. Cement leakage is considered to be the major complication. The viscosity plays a key role in this context. At high viscosity, the risk of leakage is reduced; however, injection forces are highly increased, handling time is reduced. The purpose of the study was to investigate the rheological, handling and hardening behaviour of a newly developed medium viscous bone cement at different temperatures and by simulation of a temperature shift to body-temperature. The presented data give an impression on the injectability of the cement using different sized needles. It could be concluded, that the medium viscous cement shows an adequate working time for a broad temperature range and an acceptable hardening time of around 11 min after immersing the cement into a 37• C environment.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rheological and Curing Behavior of a Newly Developed, Medium Viscous Acrylic Bone Cement

Deußer¹,

Sattig²,

Böger³

2011

ISRN Materials Science

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“…Damage to bone tissue due to heat shock is well known as the main cause of failure in osseointegration [9]. Exposing osteoblasts to a temperature of 45°C for 10 min results in a reversible disintegration of their actin filaments.…”

Section: Discussionmentioning

confidence: 99%

“…Exposing osteoblasts to a temperature of 45°C for 10 min results in a reversible disintegration of their actin filaments. Reversibility of cytoskeleton degradation is lost if the surrounding temperature is raised above 48°C [9].…”

Section: Discussionmentioning

confidence: 99%

Autografts for spinal fusion: osteogenic potential of laminectomy bone chips and bone shavings collected via high speed drill

et al. 2011

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In case of revision or minimal invasive spinal surgery, the amount of autograft possibly harvested from the lamina and the spinous processes is limited. Ekanayake and Shad (Acta Neurochir 152:651-653, 2010) suggest the application of bone shavings harvested via high speed burr additionally or instead, but so far no data regarding their osteogenic potential exist. Aim of the study was to compare the osteogenic potential of bone chips and high speed burr shavings, and to evaluate the applicability of bone shavings as an autograft for spinal fusion. Bone chips and shavings from 14 patients undergoing spinal decompression surgery were analyzed using in vitro tissue culture methods. Osteoblast emigration and proliferation, viability and mineralization were investigated and histological evaluation was performed. Bone chips from all patients showed successful osteoblast emigration after average 5.5 days. In contrast, only 57% of the corresponding bone shavings successfully demonstrated osteoblast emigration within an average time span of 14.8 days. Average osteoblast mobilisation was 1.25 9 10 6 cells per gram from bone chips and 1.73 9 10 5 cells per gram from the corresponding bone shavings. No difference was observed regarding cell viability, but population doubling times of bone chip cultures were significantly lower (50.5 vs. 121 h) and mineralization was observed in osteoblasts derived from bone chips only. Although some authors suggest the general applicability of laminectomy bone shavings as autografts for spinal fusion, autologous bone grafts obtained from laminectomy bone chips are superior in terms of cell delivery, cell proliferation and mineralization.

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“…HSPAs may be induced in periodontal ligament cells in vitro due to reoxygenation following hypoxia of hypoxia-reoxygenation stress models (12) in gingival epithelial cells with local application of low-intensity pulsed ultrasound application (19), and via heat shock in osteoblasts, (20) indicating that HSPAs play an important role in the stress adaptation of most periodontal tissues. Furthermore, the higher serum level of specific human antibodies against microbial HSPAs, but importantly not against HSPAs of human origin, tends to positively correlate with healthier periodontal tissues (21), indicating that microbial heat shock proteins are immunodominant antigens triggering adaptive immunity of the organism to contribute to protection against oral microorganisms, and to maintain gingival health (21).…”

Section: Immune Surveillance and Periodontal Healthmentioning

confidence: 99%

The Role of Molecular Chaperones (Hspas/Hsp70S) in Oral Health and Oral Inflammatory Diseases: A Review

et al. 2009

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Heat shock proteins of the 70kDa family (HSPAs/HSP70s) are major molecular chaperones and cytokines of most cells and microbes, extracellular and interstitial fluids, blood, synovial fluids and secretory body fluids like saliva. The induction of human HSPAs plays an important role at cellular level under most stress conditions; whereas microbial HSPAs improve microbial tolerance to environmental changes, and improve virulence and resistance against antimicrobial peptides. Extracellular HSPAs reveal cytoprotective properties and are involved in numerous physiological and pathological events, including modulation of cytokine release and immunity. Accordingly, HSPAs play a role in the maintenance of pulpal health, and the repair of injured dental hard tissues. HSPAs also play a role in stress adaptation of periodontal tissues, and in the maintenance of periodontal and mucosal health including defense against microbes, prevention of mucosal allergic reactions, and facilitation of healing of ulcers and wounds. Despite their advantageous effects maintaining health of several oral tissues, HSPAs are likely to play a role in the disadvantageous amplification of pulpal inflammatory response to bacteria, and in the formation of several periapical inflammatory lesions. HSPAs may also induce gingivitis under certain conditions, and play a role in the progression of periodontal bone defects. HSPAs may also play a role in atopic-type allergic reactions, autoimmune disorders, and haptenation in certain cases. Based on the above data, it can be assumed that HSPAs play an important role in oral defense under healthy conditions; however, their role is somewhat “Janus-faced” under pathological conditions.

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Heat Shock-induced Necrosis and Apoptosis in Osteoblasts

Cited by 21 publications

References 0 publications

Rheological and Curing Behavior of a Newly Developed, Medium Viscous Acrylic Bone Cement

Rheological and Curing Behavior of a Newly Developed, Medium Viscous Acrylic Bone Cement

Autografts for spinal fusion: osteogenic potential of laminectomy bone chips and bone shavings collected via high speed drill

The Role of Molecular Chaperones (Hspas/Hsp70S) in Oral Health and Oral Inflammatory Diseases: A Review

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