Heat shock factors in tomatoes: genome-wide identification, phylogenetic analysis and expression profiling under development and heat stress

Abstract

The HSF (heat shock factor) gene family contains highly conserved plant-specific transcription factors that play an important role in plant high-temperature stress responses. The present study aimed to characterize the HSF transcription factor genes in tomato (Solanum lycopersicum), which is an important vegetable crop worldwide and the model plant for fruit development studies. Twenty-six SlyHSF genes were identified in tomato, and the phylogenetic analysis showed the possible evolution profile of subgroups among in the plant kingdom. A new group O was identified that involved HSF genes in primitive plant species, like in the green algae, mosses and lycophytes. The gene structure and motifs of each SlyHSF were comprehensively analyzed. We identified orthologous, co-orthologous and paralogous HSF gene pairs in tomato, Arabidopsis and rice, and constructed a complex interaction network among these genes. The SlyHSF genes were expressed differentially in different species and at a higher level in mature fruits. The qPCR analysis was performed and showed SlyHSF genes greatly participate in plant heat tolerant pathways. Our comprehensive genome-wide analysis provided insights into the HSF gene family of tomatoes.

‘Bartlett’ pear fruit (Pyrus communis L.) ripening regulation by low temperatures involves genes associated with jasmonic acid, cold response, and transcription factors

“…In the current research, the up-regulation of a putative HsfB2b during ripening suggests a role of this TF in regulating pear ripening. This is in agreement with the results in previous research with tomato and Chinese grapevine, in which several members of Hsf family were shown to be involved in fruit ripening [61,62]. Moreover, apple HsfB2a was up-regulated during the late stage of fruit growth, when fruit reached 20 mm in diameter [63].…”

Functional characterization of maize heat shock transcription factor gene ZmHsf01 in thermotolerance

“…Several HSFs have been cloned from different species since the yeast HSF was first isolated in the 1980s (Baniwal et al, 2004;Bharti et al, 2004;Czarnecka-Verner et al, 2004;Kotak et al, 2004;Zhu et al, 2006;Guo et al, 2008;Yang et al, 2016). The first plant HSF was cloned from tomato in 1990 (Yokotani et al, 2008).…”

The Role of Major Transcription Factors in Solanaceous Food Crops under Different Stress Conditions: Current and Future Perspectives

“…Depending on the genome wide identification and characterization of heat shock transcription factor in solanaceous crops, the total lists of HSTs were listed in Table 4. By and large, Hsps has dual functions in both stress and non-stress conditions, which plainly indicates the role it has on the stress response and physiological response by regulating complex regulatory processes which was checked by the gene expression pattern to evaluate its function and tolerance to stresses [117][118][119][120][121][122][123].…”

“…The genome wide analyses of each transcription factor have been completed and "-"indicates the genome wide analysis was not done yet. Potato's WRKY [63] Dof [58], NAC [145]; ARF [192]; ERF [181] Pepper WRKY [193] ERF [194]; Dof [51] MYB [103]; ARF [190]; NAC [195] Tomato WRKY [62] Dof [53] MYB [88] HSF [120] ARF [196] ERF [171]; Eggplant's WRKY [197] Dof [52] MYB [84].…”