Heat shock factor gene family in rice: Genomic organization and transcript expression profiling in response to high temperature, low temperature and oxidative stresses

Mittal, Dheeraj; Chakrabarti, Sveta; Sarkar, Anshuk; Singh, Amanjot; Grover, Anil

doi:10.1016/j.plaphy.2009.05.003

Cited by 179 publications

(166 citation statements)

References 34 publications

(40 reference statements)

Supporting

Mentioning

152

Contrasting

Order By: Relevance

“…OsHsfA2c thus appears to be an important player in the rice heat shock response. Earlier work has documented that OsHsfA2c is one of the most rapidly induced Hsf genes in response to heat stress, that OsHsfA2c possesses transactivation activity, that OsHsfA2c shows trimer formation activity from its monomeric forms, and that OsHsfA2c binds to perfect-type HSE (Mittal et al 2009;Mittal et al 2011). This study further noted that OsHsfB4b binds with OsClpB-cyt based on yeast two-hybrid and BiFC assays.…”

Section: Discussionsupporting

confidence: 68%

“…6a, c). Our earlier work has shown that OsHsfB4b is induced in response to heat stress, OsHsfB4b lacks transactivation activity and shows trimer formation activity and maximum binding activity to perfect-type HSE in electrophoretic mobility shift assays (Mittal et al 2009;Mittal et al 2011). It may be inferred that HS-induced OsHsfA2c binds to OsClpB-cyt promoter, possibly controlling synthesis of the OsClpB-cyt transcript.…”

Section: Discussionmentioning

confidence: 93%

“…OsHsfA7, OsHsfA9, and OsHsfC1b transcripts are noted at low levels in unstressed conditions but increased upon HS and OsHsfB4c and OsHsf26 lack HS expression (Mittal et al 2009). Significant increase in downstream LacZ expression was noted from OsClpB-cyt promoter in yeast cells on transformation with OsHsfA2c only in this assay (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Genome-wide analysis has shown that rice and Arabidopsis contain 26 and 21 Hsf genes, respectively (Mittal et al 2009;Swindell et al 2007). Binding specificities of plant Hsfs to different Hsp promoters have not been much analyzed.…”

Section: Noted That the Induced Response To Hs Is Lost If The Canonicmentioning

confidence: 99%

See 3 more Smart Citations

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

Singh

Mittal

Lavania

et al. 2012

Cell Stress and Chaperones

Self Cite

View full text Add to dashboard Cite

ClpB-cytoplasmic (ClpB-cyt)/Hsp100 is an important chaperone protein in rice. Cellular expression of OsClpB-cyt transcript is governed by heat stress, metal stress, and developmental cues. Transgenic rice plants produced with 2 kb OsClpB-cyt promoter driving Gus reporter gene showed heat-and metal-regulated Gus expression in vegetative tissues and constitutive Gus expression in calli, flowering tissues, and embryonal half of seeds. Rice seedlings regenerated with OsClpB-cyt promoter fragment with deletion of its canonical heat shock element sequence (HSE −273 to −280 ) showed not only heat shock inducibility of Gus transcript/protein but also constitutive expression of Gus in vegetative tissues. It thus emerges that the only classical HSE present in OsClpB-cyt promoter is involved in repressing expression of OsClpB-cyt transcript under unstressed control conditions. Yeast onehybrid assays suggested that OsHsfA2c specifically interacts with OsClpB-cyt promoter. OsHsfA2c also showed binding with OsClpB-cyt and OsHsfB4b showed binding with OsClpB-cyt; notably, interaction of OsHsfB4b was seen for all three OsClpB/Hsp100 protein isoforms (i.e., ClpBcytoplasmic, ClpB-mitochondrial, and ClpB-chloroplastic).Furthermore, OsHsfB4b showed interaction with OsHsfA2c. This study suggests that OsHsfA2c may play a role as transcriptional activator and that OsHsfB4b is an important part of this heat shock responsive circuitry.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Noted That the Induced Response To Hs Is Lost If The Canonicmentioning

confidence: 99%

See 2 more Smart Citations

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

Singh

Mittal

Lavania

et al. 2012

Cell Stress and Chaperones

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many studies have been performed aiming to identify cis acting elements, which play a major role in transcriptional regulation (Walley et al 2007, Mittal et al 2009, Zou et al 2011. However, for the ERF family in rice, very few of such studies have been reported ( Jung et al 2010, Santos et al 2013.…”

Section: Discussionmentioning

confidence: 99%

Ethylene response factors gene regulation and expression profiles under different stresses in rice

Pegoraro¹,

Farias²,

Mertz³

et al. 2013

Theor. Exp. Plant Physiol.

View full text Add to dashboard Cite

Stresses can cause large yield reductions in cultivated plants. The response to these stresses occurs via a plethora of signalling pathways, where a large number of genes is induced or repressed. Among the environmental stress responsive genes, there are the members of the ethylene response factors (ERF) gene family. The mRNA levels of different ERF are regulated by many hormones and molecules produced under different stress conditions. In this study, with the goal of identifying the response of rice ERF genes to environmental stress, it was analysed the transcriptional expression profile of 114 of these genes under stress by anoxia, salt and Magnaporthe grisea.Also, aiming to characterize how the regulation of ERF genes occurs, the amount of known cis regulatory elements in the promoter region of these genes and their association with the expression profiles under the tested conditions were also assessed. The results indicate that some ERF members present the same specific expression profiles under different environmental stresses, while others do not. Within the ERF family, the regulation of gene expression is complex for some genes which have many cis elements in their promoters, but simple for others, demonstrating high levels of divergence among them. The findings demonstrate the importance of the study of each ERF separately, since it is not possible to establish general rules for regulation and probably for the function of these genes.

show abstract

Exploring Crossroads Between Seed Development and Stress Response

Naithani

Nonogaki

Jaiswal

2017

Mechanism of Plant Hormone Signaling Under Stress

View full text Add to dashboard Cite

Heat shock factor gene family in rice: Genomic organization and transcript expression profiling in response to high temperature, low temperature and oxidative stresses

Cited by 179 publications

References 34 publications

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

Ethylene response factors gene regulation and expression profiles under different stresses in rice

Exploring Crossroads Between Seed Development and Stress Response

Contact Info

Product

Resources

About