Heat shock factor 1 in brain tumors: a link with transient receptor potential channels TRPV1 and TRPA1

Moutafidi, Athanasia; Gatzounis, George; Zolota, Vassiliki; Assimakopoulou, Martha

doi:10.1007/s10735-021-10025-x

Cited by 4 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using paraffin immunohistochemistry, TRPV1 and TRPA1 protein expression was found in 62% of the WHO grade II astrocytomas, 37.5% of the anaplastic astrocytomas (WHO grade III), and 16.3% of GBM cases [234]. Another study using immunofluorescence also demonstrated TRPV1-like immuoreactivity in high-grade gliomas [235].…”

Section: Gliomas Including Glioblastoma Multiforme (Gbm)mentioning

confidence: 92%

“ThermoTRP” Channel Expression in Cancers: Implications for Diagnosis and Prognosis (Practical Approach by a Pathologist)

Szállaśi

2023

IJMS

View full text Add to dashboard Cite

Temperature-sensitive transient receptor potential (TRP) channels (so-called “thermoTRPs”) are multifunctional signaling molecules with important roles in cell growth and differentiation. Several “thermoTRP” channels show altered expression in cancers, though it is unclear if this is a cause or consequence of the disease. Regardless of the underlying pathology, this altered expression may potentially be used for cancer diagnosis and prognostication. “ThermoTRP” expression may distinguish between benign and malignant lesions. For example, TRPV1 is expressed in benign gastric mucosa, but is absent in gastric adenocarcinoma. TRPV1 is also expressed both in normal urothelia and non-invasive papillary urothelial carcinoma, but no TRPV1 expression has been seen in invasive urothelial carcinoma. “ThermoTRP” expression can also be used to predict clinical outcomes. For instance, in prostate cancer, TRPM8 expression predicts aggressive behavior with early metastatic disease. Furthermore, TRPV1 expression can dissect a subset of pulmonary adenocarcinoma patients with bad prognosis and resistance to a number of commonly used chemotherapeutic agents. This review will explore the current state of this rapidly evolving field with special emphasis on immunostains that can already be added to the armoire of diagnostic pathologists.

show abstract

Section: Gliomas Including Glioblastoma Multiforme (Gbm)mentioning

confidence: 92%

“ThermoTRP” Channel Expression in Cancers: Implications for Diagnosis and Prognosis (Practical Approach by a Pathologist)

Szállaśi

2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Moreover, expression levels of Beclin, LC3B, HST1, TRPV1, TRPA1, Nrf2, and DDX3X did not associate with OS of meningioma patients (p>0.05) [71,80,103,104]. Similarly, the expression of CA-IX and Bcl-2 members family (regulators of apoptosis in tumor cells) including RTRAIL-R2, RTRAIL-R4, caspase-8, cFLIP, Bak, Bcl-XL, and Mcl-1 did not associate with OS or PFS of meningioma patients (p>0.05) [55,70].…”

Section: Association Of Biomarkers and Prognosis In A Single Study Re...mentioning

confidence: 99%

Biomarkers for prognosis of meningioma patients: A systematic review and meta-analysis

Aung,

Ngamjarus,

Proungvitaya

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Meningioma is the most common primary brain tumor and many studies have evaluated numerous biomarkers for their prognostic value, often with inconsistent results. Currently, no reliable biomarkers are available to predict the survival, recurrence, and progression of meningioma patients in clinical practice. This study aims to evaluate the prognostic value of immunohistochemistry-based (IHC) biomarkers of meningioma patients. A systematic literature search was conducted up to November 2023 on PubMed, CENTRAL, CINAHL Plus, and Scopus databases. Two authors independently reviewed the identified relevant studies, extracted data, and assessed the risk of bias of the studies included. Meta-analyses were performed with the hazard ratio (HR) and 95% confidence interval (CI) of overall survival (OS), recurrence-free survival (RFS), and progression-free survival (PFS). The risk of bias in the included studies was evaluated using the Quality in Prognosis Studies (QUIPS) tool. A total of 100 studies with 16,745 patients were included in this review. As the promising markers to predict OS of meningioma patients, Ki-67/MIB-1 (HR = 1.03, 95%CI 1.02 to 1.05) was identified to associate with poor prognosis of the patients. Overexpression of cyclin A (HR = 4.91, 95%CI 1.38 to 17.44), topoisomerase II α (TOP2A) (HR = 4.90, 95%CI 2.96 to 8.12), p53 (HR = 2.40, 95%CI 1.73 to 3.34), vascular endothelial growth factor (VEGF) (HR = 1.61, 95%CI 1.36 to 1.90), and Ki-67 (HR = 1.33, 95%CI 1.21 to 1.46), were identified also as unfavorable prognostic biomarkers for poor RFS of meningioma patients. Conversely, positive progesterone receptor (PR) and p21 staining were associated with longer RFS and are considered biomarkers of favorable prognosis of meningioma patients (HR = 0.60, 95% CI 0.41 to 0.88 and HR = 1.89, 95%CI 1.11 to 3.20). Additionally, high expression of Ki-67 was identified as a prognosis biomarker for poor PFS of meningioma patients (HR = 1.02, 95%CI 1.00 to 1.04). Although only in single studies, KPNA2, CDK6, Cox-2, MCM7 and PCNA are proposed as additional markers with high expression that are related with poor prognosis of meningioma patients. In conclusion, the results of the meta-analysis demonstrated that PR, cyclin A, TOP2A, p21, p53, VEGF and Ki-67 are either positively or negatively associated with survival of meningioma patients and might be useful biomarkers to assess the prognosis.

show abstract

“…Finally, tissue sections were washed for 3 x 5 minutes with 0.1M PBS and 2 x 5 minutes in diH2O, air-dried, and covered with Vectashield Antifade Mounting Medium (Vectorlabs, Burlingame, CA, USA). In this study, the following previously characterized primary antibodies were used: anti-PGP9.5 rabbit polyclonal (Millipore-Sigma; Burlington, NJ, catalog AB1761-I; 1:300) (Boyd et al, 2021); anti-Neurofilament H (NF-H) chicken polyclonal (BioLegend; San Diego, CA; catalog 822601; 1:1000) (Avona et al, 2021); anti-CGRP guinea pig polyclonal (Synaptic Systems; Goettingen, Germany; catalog 414 004; 1:200) (Samms et al, 2022); anti-TRPV1 rabbit polyclonal (Novus Biologicals; Centennial, CO; catalog NBP1-71774SS; 1:200) (Moutafidi et al, 2021); anti-mrgprD rabbit polyclonal (Alamone Lab;…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets

Hovhannisyan

Lindquist

Belugin

et al. 2023

Preprint

View full text Add to dashboard Cite

Myogenous temporomandibular disorders (TMD-M) are the most prevalent group of painful orofacial conditions, and the second most frequent among musculoskeletal pain conditions. TMD-M is associated with an increased responsiveness of nerves innervating the masseter (MM), temporal (TM), medial pterygoid closing (MPM) and lateral pterygoid gliding muscles (LPM). Treatment of this disorder remains difficult and is further complicated by each muscle having diverse and functionally distinct nerve innervation. This study examined expression of sensory markers in MM, TM and LPM of adult male common marmosets, a type of non-human primate. Using immunohistochemistry, we found that masticatory muscles were predominantly innervated with A-fibers (NFH+). All C-fibers (pgp9.5+/NFH-) observed in masticatory muscles were peptidergic (CGRP+) and lacked antibody labeling for mrgprD, trpV1 and the silent nociceptive marker, CHRNA3. The proportion of C- to A-fibers was highest in LPM, while MM had a minimal percentage (6-8%) of C-fibers. Interestingly, C-fibers in masticatory muscle may have myelin sheath, since many NFH- nerves were labeled with GFAP+. A-fiber types were also dissimilar among these muscles. Thus, there are substantially more peptidergic A-fibers (CGRP+/NFH+) in TM and LPM compared to MM. Almost all A-fibers in MM expressed trkC, with some of them having trkB and parvalbumin (PV). In contrast, a lesser number of TM and LPM nerves expressed trkC, lacked trkB and had fewer PV+ fibers in LPM. Along with sensory fibers, the masticatory muscles contain sympathetic fibers (tyrosine hydroxylase; TH+), which are located around blood vessels. This TH expression was absent in trigeminal neurons. Overall, the masticatory muscles of male marmosets have distinct expression patterns when compared to each muscle of the jaw and cutaneous fibers innervated by DRG neurons.

show abstract

Heat shock factor 1 in brain tumors: a link with transient receptor potential channels TRPV1 and TRPA1

Cited by 4 publications

References 46 publications

“ThermoTRP” Channel Expression in Cancers: Implications for Diagnosis and Prognosis (Practical Approach by a Pathologist)

“ThermoTRP” Channel Expression in Cancers: Implications for Diagnosis and Prognosis (Practical Approach by a Pathologist)

Biomarkers for prognosis of meningioma patients: A systematic review and meta-analysis

Sensory innervation of masseter, temporal and lateral pterygoid muscles in common marmosets

Contact Info

Product

Resources

About