Heat shock effects on cell cycle progression

Kühl, Nicole M.; Rensing, Ludger

doi:10.1007/pl00000707

Cited by 120 publications

(102 citation statements)

References 118 publications

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“…Studies have indicated that heat stress induces disturbance in the percentages of chicken thymocytes in G0/G1, S, and G2/M phases (Zhang et al 2003), resulting in a large number of lymphocytes arrested in S and G2/M phases. Animals alleviate the harm of heat stress by increased cell proliferation (Kuhl et al 2000). In this study, we found that the percentage of lymphocytes in the G2/M phase of HS group was consistent with the increase in the apoptosis rate of thymocytes in doi: 10.17221/67/2015-CJAS chicks after heat stress, and both of them reached the maximum at 6 weeks of age.…”

Section: Discussionsupporting

confidence: 81%

Effects of γ-aminobutyric acid on the thymus tissue structure, antioxidant activity, cell apoptosis, and cytokine levels in chicks under heat stress

Chen¹,

Xie²,

Zhou³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:This study aims to investigate the effect of dietary γ-aminobutyric acid (GABA) on the development of thymus tissue structure and function in chicks under heat stress. One-day-old male Wenchang chicks were randomly divided into control group (CK), heat stress group (HS), and GABA+HS group. The chicks from GABA+HS group were administered 0.2 ml of GABA solution daily by oral gavage (50 mg/kg of body weight). Chicks from HS and GABA+HS groups were subjected to heat stress treatment at 40 ± 0.5°C for 2 h every day. Blood and thymus tissue were collected from the chicks at the end of weeks 1-6. Results showed that the thymus weight and index, thickness of cortex, cortex/medulla ratio, number of lymphocytes, activity of superoxide dismutase, total antioxidant capacity, and glutathione peroxidase, and plasma level of tumor necrosis factor-α in HS group were significantly lower than in CK group (P < 0.05). The Toll-like receptor 2 (TLR2) expression in the late stage of heat stress, malondialdehyde (MDA) content, thymocyte apoptosis rate, number of lymphocytes in the S and G2/M phases, and plasma levels of interleukin-4 and interferon-γ in HS group were significantly higher than in CK group (P < 0.05). In contrast, the integrity of thymus tissue structure of GABA+HS group was improved compared with HS group. The TLR2 expression in the early stage of heat stress and the activity of antioxidant enzymes in GABA+HS group were significantly higher than in HS group (P < 0.05), and the MDA content, thymocyte apoptosis rate, number of lymphocytes in the S and G2/M phases, and plasma level of IL-4 and IFN-γ in GABA+HS group were significantly lower than in HS group (P < 0.05). We concluded that heat stress caused structure damage to thymus tissue of chicks, changed the plasma levels of cytokines, reduced the antioxidant activity, and increased cell apoptosis in chick thymus. GABA alleviated the negative effects on the development of chick thymus, improved the immune function of thymus, and played a protective role by regulating the plasma levels of cytokines and antioxidant activity of thymus tissue.

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Section: Discussionsupporting

confidence: 81%

Effects of γ-aminobutyric acid on the thymus tissue structure, antioxidant activity, cell apoptosis, and cytokine levels in chicks under heat stress

Chen¹,

Xie²,

Zhou³

et al. 2016

CZECH J ANIM SCI

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“…It may be that during our two week cultivation period, explants were protected by overexpression of heat shock proteins followed by induction of growth factor expression, similarly to the findings reported in mouse tumour development 39. Moreover, a positive effect on growth was suggested to arise from the inhibition of protein synthesis of negative growth regulators 40. However, at the end of culture, the ability for cell proliferation was lower than in controls, which can be explained by the fact that terminal differentiation that we find occurs after the exit from the cell cycle.…”

Section: Discussionsupporting

confidence: 79%

Influence of hyperthermal regimes on experimental teratoma development in vitro

Bojanac

Rogosic

Sinčić

et al. 2018

Int J Experimental Path

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SummaryWe screened for the impact of hyperthermal regimes varying in the cumulative equivalent minutes at 43°C (CEM43°C) and media composition on tumour development using an original teratoma in vitro model. Rat embryos (three germ layers) were microsurgically isolated and cultivated at the air‐liquid interface. During a two week period, ectodermal, mesodermal and endodermal derivatives developed within trilaminar teratomas. Controls were grown at 37°C. Overall growth was measured, and teratoma survival and differentiation were histologically assessed. Cell proliferation was stereologically quantified by the volume density of Proliferating Cell Nuclear Antigen. Hyperthermia of 42°C, applied for 15 minutes after plating (CEM43°C 3.75 minutes), diminished cell proliferation (P ˂ .0001) and enhanced differentiation of both myotubes (P ˂ .01) and cylindrical epithelium (P ˂ .05). Hyperthermia of 43°C applied each day for 30 minutes during the first week (CEM43°C 210 minutes) impaired overall growth (P ˂ .01) and diminished cell proliferation (P ˂ .0001). Long‐term hyperthermia of 40.5°C applied for two weeks (CEM43°C 630 minutes) significantly impaired survival (P ˂ .005). Long‐term hyperthermia of 40.5°C applied from the second day when differentiation of tissues begins (CEM43°C 585 minutes) impaired survival (P ˂ .0001), overall growth (P ˂ .01) and cartilage differentiation (P ˂ .05). No teratomas survived extreme regimes: 43°C for 24 hours (CEM43°C 1440 minutes), hyperthermia in the scant serum‐free medium (CEM43°C 630 minutes) or treatment with an anti‐HSP70 antibody before long‐term hyperthermia 40.5°C from the second day (CEM43°C 585 minutes). This in vitro research provided novel insights into the impact of hyperthermia on the development of experimental teratomas from their undifferentiated sources and are thus of potential interest for future therapeutic strategies in corresponding in vivo models.

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“…As the distribution of 1n, 2n, and 4n cells remained almost constant during HS ( Figure 2B), cells apparently were cell cycle arrested in both G1 (unreplicated DNA = 1n) and G2 phases (after DNA replication $ 2n, 4n), perhaps as a consequence of reduced activities of cyclin-dependent kinases (Kühl and Rensing, 2000). As presumably in all eukaryotes, including Chlamydomonas, the (A) Behavior of TAG clusters.…”

Section: Cells Undergo An Immediate Cell Cycle Arrest Upon Onset Of Hsmentioning

confidence: 82%

Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model OrganismChlamydomonas reinhardtii

et al. 2014

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We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42°C for 24 h and back to 25°C for ‡8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and photosynthesis parameters. Our data indicate that acclimation of Chlamydomonas to long-term HS consists of a temporally ordered, orchestrated implementation of response elements at various system levels. These comprise (1) cell cycle arrest; (2) catabolism of larger molecules to generate compounds with roles in stress protection; (3) accumulation of molecular chaperones to restore protein homeostasis together with compatible solutes; (4) redirection of photosynthetic energy and reducing power from the Calvin cycle to the de novo synthesis of saturated fatty acids to replace polyunsaturated ones in membrane lipids, which are deposited in lipid bodies; and (5) when sinks for photosynthetic energy and reducing power are depleted, resumption of Calvin cycle activity associated with increased photorespiration, accumulation of reactive oxygen species scavengers, and throttling of linear electron flow by antenna uncoupling. During recovery from HS, cells appear to focus on processes allowing rapid resumption of growth rather than restoring pre-HS conditions.

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Heat shock effects on cell cycle progression

Cited by 120 publications

References 118 publications

Effects of γ-aminobutyric acid on the thymus tissue structure, antioxidant activity, cell apoptosis, and cytokine levels in chicks under heat stress

Effects of γ-aminobutyric acid on the thymus tissue structure, antioxidant activity, cell apoptosis, and cytokine levels in chicks under heat stress

Influence of hyperthermal regimes on experimental teratoma development in vitro

Systems-Wide Analysis of Acclimation Responses to Long-Term Heat Stress and Recovery in the Photosynthetic Model OrganismChlamydomonas reinhardtii

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