Heat shock during rat embryo development in vitro results in decreased mitosis and abundant cell death

Breen, John G.; Claggett, T.W; Kimmel, Gary L.; Kimmel, Carole A.

doi:10.1016/s0890-6238(98)00056-2

Cited by 33 publications

(25 citation statements)

References 22 publications

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“…6a). These results confirm what has been seen in a number of terrestrial animals (Blagden et al 1997;Breen et al 1999) but not in fish, namely, that HS during somitogenesis induces vertebral deformities. It has also been suggested that the location of deformity is dependent on the fact that HS disturbs a cyclic code ("segmental clock") that patterns the embryonic rostro-caudal axis in an anterior-posterior direction (Dale et al 2003;Maroto and Pourquie 2001).…”

Section: Discussionsupporting

confidence: 91%

“…Vertebral deformities are induced at high egg-incubation temperatures in a number of fish species (Sfakianakis et al 2004; Wang and Tsai 2000;Wiegand et al 1989), among them Atlantic salmon (Vagsholm and Djupvik 1998). In higher vertebrates such as rats and mice, it has been shown that sudden temperature elevations during early axis formation can induce vertebral deformities (Blagden et al 1997;Breen et al 1999). These results imply that the induction of the somite (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Heat shock during early somitogenesis induces caudal vertebral column defects in Atlantic salmon (Salmo salar)

2005

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In several terrestrial vertebrates, heat shock (HS) during somitogenesis causes vertebral deformities. To determine if vertebral deformities can occur due to sudden temperature changes during early development in fish, Atlantic salmon embryos were HS treated during somitogenesis. Ten months later these individuals displayed a high prevalence of caudal vertebral column condensations (27-34%). The defects were located caudally of the abdominal cavity, displaying an even distribution in this region independent of time of HS. To determine if HS disturbed vertebral development during somitogenesis, two genes coding for markers of skeletal development were identified, namely, the secreted protein Shh (Sashh) and the transcription factor Twist (Satwist). These proteins are involved in the proliferation and specification of presumptive skeletal cells (sclerotome) in vertebrates. The spatial expression pattern of sashh and satwist in salmon indicated a functional conservation of these proteins. Furthermore, HS embryos displayed expressional disturbance in both sashh and satwist, indicating an effect of HS on sclerotomal cell patterning. However, the HS-protecting ability in embryos seems to be individually regulated because reduction in gene expression was not detected at all stages; in addition, HS did not induce somitic disturbance and vertebral deformity in all embryos.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Heat shock during early somitogenesis induces caudal vertebral column defects in Atlantic salmon (Salmo salar)

2005

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“…This was also the finding by other labs that heat shocked embryos in culture (Mirkes, 1985;Walsh et al, 1987). As observed in vivo, these effects were time-and temperature-dependent (Walsh and Morris, 1989;Breen et al, 1999). Therefore, the cell death that was observed was the predominate effect of heat in embryos in vitro and in vivo.…”

Section: Mechanistic Observationssupporting

confidence: 80%

Hyperthermia: malformations to chaperones

Bennett

2010

Birth Defects Research Pt B

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Hyperthermia has been known to induce malformations in numerous animal models as well being associated with human abnormalities. This was apparent particularly when the hyperthermia exposure was during the early stages of neural development. Although it was recognized relatively early that these exposures induced cell death, the specific molecular mechanism of how a brief heat exposure was translated in to specific cellular functions remains largely unknown. While our understanding of the events that govern how cells react to heat, or stresses in general, has increased, there is much that remains undiscovered. In this brief review, animal and clinical observations are outlined as are some of the scientific explorations that were undertaken to characterize, define, and better understand the morphological, biochemical, and molecular effects of hyperthermia on the developing embryo.

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“…Studies on CHO or Hela cells report a chronic tolerance and a progression through cell cycle during chronic heating at an even higher temperature, 41.5°C 72. The negative effect on cartilage development could be related to changes in the Hox gene expression 19 and skeletal development 55, 73. It was suggested that a single acute exposure to 42°C be a threshold dose in skeletal development 17.…”

Section: Discussionmentioning

confidence: 99%

Influence of hyperthermal regimes on experimental teratoma development in vitro

Bojanac

Rogosic

Sinčić

et al. 2018

Int J Experimental Path

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SummaryWe screened for the impact of hyperthermal regimes varying in the cumulative equivalent minutes at 43°C (CEM43°C) and media composition on tumour development using an original teratoma in vitro model. Rat embryos (three germ layers) were microsurgically isolated and cultivated at the air‐liquid interface. During a two week period, ectodermal, mesodermal and endodermal derivatives developed within trilaminar teratomas. Controls were grown at 37°C. Overall growth was measured, and teratoma survival and differentiation were histologically assessed. Cell proliferation was stereologically quantified by the volume density of Proliferating Cell Nuclear Antigen. Hyperthermia of 42°C, applied for 15 minutes after plating (CEM43°C 3.75 minutes), diminished cell proliferation (P ˂ .0001) and enhanced differentiation of both myotubes (P ˂ .01) and cylindrical epithelium (P ˂ .05). Hyperthermia of 43°C applied each day for 30 minutes during the first week (CEM43°C 210 minutes) impaired overall growth (P ˂ .01) and diminished cell proliferation (P ˂ .0001). Long‐term hyperthermia of 40.5°C applied for two weeks (CEM43°C 630 minutes) significantly impaired survival (P ˂ .005). Long‐term hyperthermia of 40.5°C applied from the second day when differentiation of tissues begins (CEM43°C 585 minutes) impaired survival (P ˂ .0001), overall growth (P ˂ .01) and cartilage differentiation (P ˂ .05). No teratomas survived extreme regimes: 43°C for 24 hours (CEM43°C 1440 minutes), hyperthermia in the scant serum‐free medium (CEM43°C 630 minutes) or treatment with an anti‐HSP70 antibody before long‐term hyperthermia 40.5°C from the second day (CEM43°C 585 minutes). This in vitro research provided novel insights into the impact of hyperthermia on the development of experimental teratomas from their undifferentiated sources and are thus of potential interest for future therapeutic strategies in corresponding in vivo models.

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Heat shock during rat embryo development in vitro results in decreased mitosis and abundant cell death

Cited by 33 publications

References 22 publications

Heat shock during early somitogenesis induces caudal vertebral column defects in Atlantic salmon (Salmo salar)

Heat shock during early somitogenesis induces caudal vertebral column defects in Atlantic salmon (Salmo salar)

Hyperthermia: malformations to chaperones

Influence of hyperthermal regimes on experimental teratoma development in vitro

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