Heat shock and structural proteins associated with meat tenderness in Nellore beef cattle, a Bos indicus breed

Carvalho, Minos Esperândio; Gasparin, G.; Poleti, Mirele Daiana; Rosa, Alessandra Fernandes; Balieiro, J. C. C.; Labate, Carlos Alberto; Nassu, R. T.; Túllio, R. R.; Regitano, Luciana Correia de Almeida; Mourão, Gerson Barreto; Coutinho, Luiz Lehmann

doi:10.1016/j.meatsci.2013.11.014

Cited by 86 publications

(65 citation statements)

References 32 publications

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“…TNNT3 [15] and TPM1 [22] have been more abundant in tough beef. These findings are in agreement with our results, because Nellore muscle showed greater abundance of TPM1 and TNNT3 compared to Angus.…”

Section: Resultsmentioning

confidence: 99%

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Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

et al. 2017

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Proteins are the major constituents of muscle and are key molecules regulating the metabolic changes during conversion of muscle to meat. Brazil is one of the largest exporters of beef and most Brazilian cattle are composed by zebu (Nellore) genotype. Bos indicus beef is generally leaner and tougher than Bos taurus such as Angus. The aim of this study was to compare the muscle proteomic and phosphoproteomic profile of Angus and Nellore. Seven animals of each breed previously subjected the same growth management were confined for 84 days. Proteins were extracted from Longissimus lumborum samples collected immediately after slaughter and separated by two-dimensional electrophoresis. Pro-Q Diamond stain was used in phosphoproteomics. Proteins identification was performed using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Tropomyosin alpha-1 chain, troponin-T, myosin light chain-1 fragment, cytoplasmic malate dehydrogenase, alpha-enolase and 78 kDa glucose-regulated protein were more abundant in Nellore, while myosin light chain 3, prohibitin, mitochondrial stress-70 protein and heat shock 70 kDa protein 6 were more abundant in Angus (P<0.05). Nellore had higher phosphorylation of myosin regulatory light chain-2, alpha actin-1, triosephosphate isomerase and 14-3-3 protein epsilon. However, Angus had greater phosphorylation of phosphoglucomutase-1 and troponin-T (P<0.05). Therefore, proteins involved in contraction and muscle organization, myofilaments expressed in fast or slow-twitch fibers and heat shock proteins localized in mitochondria or sarcoplasmic reticulum and involved in cell flux of calcium and apoptosis might be associated with differences in beef quality between Angus and Nellore. Furthermore, prohibitin appears to be a potential biomarker of intramuscular fat in cattle. Additionally, differences in phosphorylation of myofilaments and glycolytic enzymes could be involved with differences in muscle contraction force, susceptibility to calpain, apoptosis and postmortem glycolysis, which might also be related to differences in beef quality among Angus and Nellore.

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Section: Resultsmentioning

confidence: 99%

“…Several studies have found a relationship between meat tenderness and HSPs abundance [22, 36, 37]. Due to the highly conserved chaperone capacity of proteins belonging to the HSPs family, many research groups have discussed the involvement of HSPs in the meat tenderizing process, without considering their singularities.…”

Section: Resultsmentioning

confidence: 99%

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

et al. 2017

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“…Some of these proteins were also identified as biomarkers of tenderness in the literature by several authors, e.g. Park7, PRDX6 (Jia et al, 2009), and several Hsp (Carvalho et al, 2014;Lomiwes, Farouk, Wiklund, & Young, 2014). Parvalbumin and annexin A6 are involved in calcium metabolism, or galectin (Hollung et al (2007); Bjarnadottir et al (2012)).…”

Section: Biomarkers Discoverymentioning

confidence: 98%

Recent advances in omic technologies for meat quality management

et al. 2015

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“…Moreover, PDF1 cooperates with sHSPs like HSP70 and HSP40 (GEISSLER; SIEGERS; SCHIEBEL, 1998;HAYER-HARTL, 2002;SIEGERS et al, 1999;VAINBERG et al, 1998). The HSP70 and other several sHSPs were previously associated with beef tenderness (LOMIWES et al, 2014), including this Nelore population (CARVALHO et al, 2014).…”

Section: Cnvr 760mentioning

confidence: 98%

“…The SF values were used to estimate genetic breeding values for meat tenderness (MT-EBV, detailed procedure in (CARVALHO et al, 2014)). A total of 250 animals with MT-EBV values were used herein.…”

Section: Phenotype and Mt-ebv Estimationmentioning

confidence: 99%

Identification of CNVs in the Nelore genome and its association with meat tenderness

Silva¹

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Heat shock and structural proteins associated with meat tenderness in Nellore beef cattle, a Bos indicus breed

Cited by 86 publications

References 32 publications

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

Differences in Beef Quality between Angus (Bos taurus taurus) and Nellore (Bos taurus indicus) Cattle through a Proteomic and Phosphoproteomic Approach

Recent advances in omic technologies for meat quality management

Identification of CNVs in the Nelore genome and its association with meat tenderness

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