HEAT RESISTANT BACTERIA IN RAW MILK Part 111. Occurrence of Thermoduric Bacteria in Farm Milk Supplies

Thomas, S. B.; Griffiths, D. G.; Thomas, Blodwen F.; Humphreys, Mervyn O.; Ellison, Dorothy

doi:10.1111/j.1471-0307.1950.tb01935.x

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Bulking of farm milk supplies a t country creameries usually resulted in the majority of the cooled, bulk tanker supplies of 2000-3000 gal examined during 1949 before despatch to pasteurizing dairies, having a thermoduric organism's content of lo4-105/ml, counts of 103/ml being rare, except where the intake was subject to rigorous bacteriological control (Thomas, Hobson, Griffiths, George & Jenkins, 1951).…”

Section: Incidence Of Thermoduric Bacteria In Farmmentioning

confidence: 99%

Incidence and Significance of Thermoduric Bacteria in Farm Milk Supplies: A Reappraisal and Review

Thomas¹,

Druce²,

Peters

et al. 1967

Journal of Applied Bacteriology

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CONTENTS PAGE 26'i r i 1 hermoduric bacteria in farm milk supplies miportance of using a suitable brand of peptone in the plating medium, w liich should be at a pH of 7.5, while iiicubation should preferably be a t 28" for 96 h. The results of these findings should be incorporated in future revisions of standard techniques. Donovan & Vincent (1955) recommended the use of a double indicator (broniocresol purple and phenol red) agar medium for estimating the numbers and types of thermodurics, while Leali (1958) used a TTC medium which he claimed facilitated and reduced the error of counting colonies of thermoduric bacteria.

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Section: Incidence Of Thermoduric Bacteria In Farmmentioning

confidence: 99%

Incidence and Significance of Thermoduric Bacteria in Farm Milk Supplies: A Reappraisal and Review

Thomas¹,

Druce²,

Peters

et al. 1967

Journal of Applied Bacteriology

Self Cite

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Section B. Bacteriology and mycology applied to dairying

Hiscox¹,

Briggs²

1953

Journal of Dairy Research

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Reviews of the progress of dairy sciencepreformed enzymes, are added to chemically defined substrates, giving clear-cut results. A clockwork automatic bacteriological inoculating device has been devised by Fuson & Marcus (20). An ingenious device(2i), patented in the U.S.A., for counting bacterial colonies, consists of a fountain pen connected to an electro-magnetic counter which registers each time the pen touches the Petri dish or roll-tube. Hannay (22) has described a new type of laterally illuminated colony counting chamber. British Standards for Petri dishes (23) have been issued recently. A microtechnique for the growth of many cultures, particularly for assay work, in one Petri dish has been developed by Turner-Graff(24). Newbould's(25) roll-tube spinner is designed to use cheap, easily obtainable vials or bottles. A suggestion contributing, in the long run, to economy is that of using rigid, seamless tubes of Kraft paper instead of the paper wrappings commonly used for individual sterile pipettes (26); Lack (27) has described a hot box for microcultures, which dispenses with a water jacket since the heating element consists of glass electrothermal heating tape. From America have come details of a simple large-scale anaerobic culture apparatus (28) utilizing a milk can as the main container, and of the uses to which spun glass (29) may be put as an air-filtering medium for bacteriological purposes.The text of the papers read, and the discussion, at a Symposium of the Institute of Biology in London in 1951 have been published under the title Freezing and Drying^); diversity of opinion and lack of collaboration and understanding between bacteriologist and vacuum engineer was apparent concerning even such vital factors as the plugs for drying tubes. Fry & Greaves (31), working mainly with a paracolon bacillus over a 3-year period, have shown that the nature of the suspending medium is one of the most important factors determining the survival rate of bacteria dried in vacuo; the addition of 5-10% of glucose or lactose increased the percentage survival. Young cultures were less resistant to drying than older cultures, but the cell concentration appeared to be of little consequence. ' Mist desiccans' (1 part broth, 3 parts serum with 7-5% added glucose) proved a satisfactory suspending medium for sensitive organisms such as Neisseria gonorrhoea. Briggs' observations (32) on the freeze-drying of lactobacilli are in almost complete agreement with those of Fry & Greaves; using strains of Lactobacillus acidophilus and L. bulgaricus, which were particularly sensitive to freeze-drying, she obtained with her suspending media survival rates of over 10% after 18 months' storage; her work is being continued with other species of lactobacilli subsequently tested to determine whether selection has taken place, although this should not be a danger except where very low survival rates are experienced. Sharpe (33) has tested the precipitin and agglutination reactions of cultures of L. acidophilus and L. bulgaricus which had b...

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Quality of manufacturing-grade bulk-tank milk

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HEAT RESISTANT BACTERIA IN RAW MILK Part 111. Occurrence of Thermoduric Bacteria in Farm Milk Supplies

Cited by 3 publications

References 10 publications

Incidence and Significance of Thermoduric Bacteria in Farm Milk Supplies: A Reappraisal and Review

Incidence and Significance of Thermoduric Bacteria in Farm Milk Supplies: A Reappraisal and Review

Section B. Bacteriology and mycology applied to dairying

Quality of manufacturing-grade bulk-tank milk

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