Heat resistance of Clostridium sordellii spores

Kozma-Sipos, Zs.; Szigeti, Jenő; Ásványi, Balázs; Varga, László

doi:10.1016/j.anaerobe.2010.01.002

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…In this study, we identified C. tetani in 6.25% of isolates. Clostridium sordellii is an uncommon human pathogen but virulent strains cause fatal infections in a wide range of animals, such as cattle and sheep (Kozma-Sipos et al 2010;Vidor et al 2015). In this study, we found C. sordellii in 7.5% of isolates.…”

Section: Discussionmentioning

confidence: 48%

Identification of Clostridium spp. derived from a sheep and cattle slaughterhouse by matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing

et al. 2018

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Using a cut-off score of 1.7 was sufficient for accurate identification of species. MALDI-TOF MS identification scores for spp. decreased with longer incubation time. species predominantly were isolated from carcasses after skinning and evisceration steps in the slaughterhouse. MALDI-TOF MS could be an accurate way to identify species. Moreover, continuous improvement of the database and MALDI-TOF MS instrument enhance its performance in food control laboratories.

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Section: Discussionmentioning

confidence: 48%

Identification of Clostridium spp. derived from a sheep and cattle slaughterhouse by matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing

et al. 2018

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“…Four‐log 10 reduction times are frequently used in disinfection studies (Kozma‐Sipos et al. ; Oliveira et al. ; Cho et al.…”

Section: Methodsmentioning

confidence: 99%

The differential susceptibility of spores from virulent and attenuated Bacillus anthracis strains to aldehyde‐ and hypochlorite‐based disinfectants

et al. 2012

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This study compared the sensitivity of spores from virulent and attenuated Bacillus anthracis strains in suspension to inactivation by various chemical disinfectants. Spore suspensions from two virulent strains (A0256 and A0372) and two attenuated strains (Sterne and A0141) of B. anthracis were tested against two aldehyde-based disinfectants and one hypochlorite-based disinfectant. A novel statistical model was used to estimate 4-log10 reduction times for each disinfectant/strain combination. Reduction times were compared statistically using approximate Z and χ2 tests. Although there was no consistent correlation between virulence and increased sporicidal resistance across all three disinfectants, spores from the two virulent and two attenuated strains did display significantly different susceptibilities to different disinfectants. Significant disinfectant–strain interactions were observed for two of the three disinfectants evaluated. The comparative results suggest that the use of surrogate organisms to model the inactivation kinetics of virulent B. anthracis spores may be misleading. The accuracy of such extrapolations is disinfectant dependent and must be used with caution.

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“…In this study, the contamination of mobile medical equipment such as stretchers, drip stands, mayo tables, instrumental tables, work tables, and trolleys was remarkable; these devices served as potential vectors in spore dissemination in the hospital environment [ 29 ]. Ten percent of P. sordellii isolates were recovered from cleaning and disinfection equipment and sterile surgical instruments, probably due to intrinsic spore resistance to biocides and high temperatures [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Paeniclostridium sordellii in Hospital Settings

et al. 2021

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(1) Background: The purpose of this study was to determine the prevalence of clostridia strains in a hospital environment in Algeria and to evaluate their antimicrobial susceptibility to antibiotics and biocides. (2) Methods: Five hundred surface samples were collected from surfaces in the intensive care unit and surgical wards in the University Hospital of Tlemcen, Algeria. Bacterial identification was carried out using MALDI-TOF-MS, and then the minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by the E-test method. P. sordellii toxins were searched by enzymatic and PCR assays. Seven products intended for daily disinfection in the hospitals were tested against Clostridium spp. spore collections. (3) Results: Among 100 isolates, 90 P. sordellii were identified, and all strains were devoid of lethal and hemorrhagic toxin genes. Beta-lactam, linezolid, vancomycin, tigecycline, rifampicin, and chloramphenicol all proved effective against isolated strains. Among all strains tested, the spores of P. sordellii exhibited remarkable resistance to the tested biocides compared to other Clostridium species. The (chlorine-based 0.6%, 30 min), (glutaraldehyde solution 2.5%, 30 min), and (hydrogen peroxide/peracetic acid 3%, 15 min) products achieved the required reduction in spores. (4) Conclusions: Our hospital’s current cleaning and disinfection methods need to be optimized to effectively remove spores from caregivers’ hands, equipment, and surfaces.

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Heat resistance of Clostridium sordellii spores

Cited by 3 publications

References 18 publications

Identification of Clostridium spp. derived from a sheep and cattle slaughterhouse by matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing

Identification of Clostridium spp. derived from a sheep and cattle slaughterhouse by matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing

The differential susceptibility of spores from virulent and attenuated Bacillus anthracis strains to aldehyde‐ and hypochlorite‐based disinfectants

Prevalence and Antimicrobial Resistance of Paeniclostridium sordellii in Hospital Settings

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