Heat Production. ATP Concentration and Electron Transport Activity of Marine Sediments

Pamatmat, MM; Graf, Gerhard; Bengtsson, W.; Novak, C. F.

doi:10.3354/meps004135

Cited by 61 publications

(27 citation statements)

References 12 publications

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“…Triton X100 solubilizes the formazan formed in the mixture assay and avoids the need for an organic extraction; it also strongly increases enzyme activity by improving the solubilization of membrane-bonded enzyme complexes. The method has been widely applied to plankton samples (see Packard 1985a, b) but less frequently to marine sediments (Wieser & Zech 1976, Christensen & Packard 1977, Olanczuk-Neyman & Voslan 1977, Pamatmat et al 1981, Christensen 1983, Pfanrlkuche et al 1983, de Wilde et al 1986) and freshwater sediments (Zimmerman 1975, Jones & Simon 1979, Broberg 1985. Various applications of the method can be found in Vosjan (1982).…”

Section: Introductionmentioning

confidence: 99%

Measurement of the respiratory electron transport system (ETS) activity in marine sediments:state-of-the-art and interpretation. I. Methodology and review of literature data

Jc¹

1996

Mar. Ecol. Prog. Ser.

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Respiration, the biochemical transfer of electrons along respiratory chains, provides energy for maintenance and growth. Measurements of the activity of the respiratory electron transport system (ETS) have been adapted for marine studies since the 1970s. In this paper, the application of ETS activity measurements to marine sediment samples is discussed and a review of literature data is presented. From a technical point of view, extraction efficiency of the respiratory units during homogenization is the main difficulty in applying the method to sediment. It has been recognized that timeand temperature-controlled ultrasonic disruption is the most efficient extractionmethod. The dilution of the homogenate is also of critical importance for efficient recovery of ETS activity. A new, accurate method using microtitration techniques is given for laboratory and shipboard applications. Published data show that ETS activity in marine surface sediments, expressed at 20°C, decreases strongly from coastal zones (mean: 186 1. 11 O2 h-' g-l) to the shelf (38 p1 0, h-' g-l) and to deep sea areas (less than 5 p1

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Section: Introductionmentioning

confidence: 99%

Measurement of the respiratory electron transport system (ETS) activity in marine sediments:state-of-the-art and interpretation. I. Methodology and review of literature data

Jc¹

1996

Mar. Ecol. Prog. Ser.

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“…ETS measurements can be used not only to calculate F C , NRE, and HEP, but also to calculate biological heat production (Pamatmat et al, 1981), age, and flow rates of deep and bottom waters (Packard, 1985a). In anoxic waters, if the background chemistry (Richards, 1965) is known, ETS measurements provide proxy rate measurements for denitrification (Packard, 1969;Codispoti and Packard, 1980;Dalsgaard et al, 2012), NO − 2 production, nitrous oxide production, and sulfide production (Packard et al, 1983), and even for iron…”

Section: Discussionmentioning

confidence: 99%

Peruvian upwelling plankton respiration: calculations of carbon flux, nutrient retention efficiency, and heterotrophic energy production

et al. 2015

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Abstract. Oceanic depth profiles of plankton respiration are described by a power function, R CO 2 = (R CO 2 ) 0 (z/z 0 ) b , similar to the vertical carbon flux profile. Furthermore, because both ocean processes are closely related, conceptually and mathematically, each can be calculated from the other. The exponent b, always negative, defines the maximum curvature of the respiration-depth profile and controls the carbon flux. When |b| is large, the carbon flux (F C ) from the epipelagic ocean is low and the nutrient retention efficiency (NRE) is high, allowing these waters to maintain high productivity. The opposite occurs when |b| is small. This means that the attenuation of respiration in ocean water columns is critical in understanding and predicting both vertical F C as well as the capacity of epipelagic ecosystems to retain their nutrients. The ratio of seawater R CO 2 to incoming F C is the NRE, a new metric that represents nutrient regeneration in a seawater layer in reference to the nutrients introduced into that layer via F C . A depth profile of F C is the integral of water column respiration. This relationship facilitates calculating ocean sections of F C from water column respiration. In an F C section and in a NRE section across the Peruvian upwelling system we found an F C maximum and a NRE minimum extending down to 400 m, 50 km off the Peruvian coast over the upper part of the continental slope. Finally, considering the coupling between respiratory electron transport system activity and heterotrophic oxidative phosphorylation promoted the calculation of an ocean section of heterotrophic energy production (HEP). It ranged from 250 to 500 J d −1 m −3 in the euphotic zone to less than 5 J d −1 m −3 below 200 m on this ocean section.

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“…The method, being optimised for oxygen respiration, overestimates real oxygen respiration by a factor 5 to 7 (Chnstensen & Packard 1979, Pamatmat et al 1981. Whether sulfate respiration is underestimated or overestimated is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…ATP and ETS-activity were analysed according to Pamatmat et al (1981). A more detailed description of the ETS-method, especially for anoxic sediments, is given by Graf & Bengtsson (1984).…”

Section: Rv Littonna Cruisesmentioning

confidence: 99%

Winter inversion of biomass and activity profile in a marine sediment

Graf¹

1986

Mar. Ecol. Prog. Ser.

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Analysis of marine sedlrnent from fie1 Bight (Baltic Sea) revealed increasing ETS-activity and heat production with increasing sediment depth down to 4 to 7 cm. A subsurface ATP-biomass maximum was found immediately above the Eh = +l00 mV layer in the sediment. This unusual situation is interpreted in terms of a better adaptation to low temperature by sulfate-respiring bacteria, compared to aerobic organisms in the sediment surface. The sediment showed high oxygen consumption but undetectable respiratory activity as measured by ETS-activity.

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Heat Production. ATP Concentration and Electron Transport Activity of Marine Sediments

Cited by 61 publications

References 12 publications

Measurement of the respiratory electron transport system (ETS) activity in marine sediments:state-of-the-art and interpretation. I. Methodology and review of literature data

Measurement of the respiratory electron transport system (ETS) activity in marine sediments:state-of-the-art and interpretation. I. Methodology and review of literature data

Peruvian upwelling plankton respiration: calculations of carbon flux, nutrient retention efficiency, and heterotrophic energy production

Winter inversion of biomass and activity profile in a marine sediment

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