Heat‐killed L actobacillus brevis KB290 attenuates visceral fat accumulation induced by high‐fat diet in mice

Abstract

Aims: This study aimed to evaluate the anti-adiposity effect of heat-killed Lactobacillus brevis KB290 originating from traditional Japanese fermented pickles in mice fed a high-fat diet (HFD). Methods and Results: C57BL/6J mice were fed a normal-fat diet, HFD or HFD supplemented with heat-killed KB290 for 8 weeks. Epididymal and renal adipose tissue weights, as well as areas of epididymal adipocytes, were significantly lower in the mice fed a HFD supplemented with KB290 than in those fed an unsupplemented HFD. Mice whose diets were supplemented with KB290 had elevated adiponectin and b3-adrenergic receptor expression in epididymal adipose tissue and an accompanying higher serum free fatty acid level. Furthermore, the HFD-induced elevations in serum glucose, insulin and HOMA-IR were significantly suppressed by dietary supplementation with KB290. Amplicon sequencing of 16S rRNA genes revealed that KB290 ingestion altered the composition of the intestinal microbiota. Conclusions: Heat-killed L. brevis KB290 suppressed diet-induced visceral fat accumulation and ameliorated diet-induced metabolic symptoms and intestinal gut microbiota modifications, suggesting possibility of novel paraprobiotic. Significance and Impact of the Study: Heat-killed L. brevis KB290 is useable as a material to develop functional foods that attenuate visceral fat accumulation.

Heat-Killed Lactiplantibacillus plantarum LRCC5314 Mitigates the Effects of Stress-Related Type 2 Diabetes in Mice via Gut Microbiome Modulation

“…Therefore, since our research was conducted in chronic cold-stress exposure, BNR17 may show increased body weight. The abundance of beneficial bacteria in the intestine is increased by probiotics, which changes the composition of the intestinal microbiota and has a potential beneficial effect against T2D by affecting energy and glucose metabolism and insulin sensitivity [ 57 , 58 ]. These results suggest that HK-LRCC5314 treatment may have beneficial effects on the glucose metabolism of mice with stress-T2D.…”

Lactobacillus rhamnosus 069 and Lactobacillus brevis 031: Unraveling Strain-Specific Pathways for Modulating Lipid Metabolism and Attenuating High-Fat-Diet-Induced Obesity in Mice

“…Further, our exploration of gut microbiota composition revealed significant alterations induced by these strains, underscoring their ability to modulate the microbiome and improve metabolic health, resonate with the outcomes from heat-killed Lactobacillus brevis KB290, showing reduced visceral fat accumulation and modulated gut microbiota in mice. 67 Notable examples include the administration of Bifidobacterium pseudocatenulatum CECT 7765, which exhibited significant anti-inflammatory properties in obese mice subjected to a high-fat diet. 68 The probiotic formulation VSL#3, comprising Lactobacillus and Bifidobacteria strains, showed therapeutic potential for enhancing insulin sensitivity and suppressing body weight gain in both mouse models and human trials.…”

Postbiotics against Obesity: Perception and Overview Based on Pre-Clinical and Clinical Studies

“…Another study documented the anti-obesity effect of heat-killed Lactobacillus brevis KB290 in HFD-fed obese mice, indicating a paraprobiotic role for this bacterium. Mice in the paraprobiotic group showed a higher abundance of Bacteroides than that in the other groups [ 134 ] ( Table 1 ).…”