Heat inactivation of monkeypox virus

Batéjat, Christophe; Grassin, Quentin; Feher, Maxence; Hoinard, Damien; Vanhomwegen, Jessica; Manuguerra, Jean-Claude; Leclercq, India

doi:10.1016/j.jobb.2022.08.001

Cited by 15 publications

(14 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, our work is one of the first in testing inactivation protocols specifically against MPXV culture isolates with widely available material and easy setup. Batéjat et al also recently reported heat inactivation against MPXV, supporting our findings, and even demonstrating inactivation at 70°C for less than 5 min 15 …”

Section: Discussionsupporting

confidence: 92%

Rapid and reliable inactivation protocols for the diagnostics of emerging viruses: The example of SARS‐CoV‐2 and monkeypox virus

Quéromès

Bouscambert‐Duchamp

Oblette

et al. 2022

Journal of Medical Virology

View full text Add to dashboard Cite

The emergence and sustained transmission of novel pathogens are exerting an increasing demand on the diagnostics sector worldwide, as seen with the ongoing severe acute respiratory coronavirus 2 (SARS‐CoV‐2) pandemic and the more recent public health concern of monkeypox virus (MPXV) since May 2022. Appropriate and reliable viral inactivation measures are needed to ensure the safety of personnel handling these infectious samples. In the present study, seven commercialized diagnosis buffers, heat (56°C and 60°C), and sodium dodecyl sulfate detergent (2.0%, 1.0%, and 0.5% final concentrations) were tested against infectious SARS‐CoV‐2 and MPXV culture isolates on Vero cell culture. Cytopathic effects were observed up to 7 days postinoculation and viral load evolution was measured by semiquantitative polymerase chain reaction. The World Health Organization recommends an infectious titer reduction of at least 4 log 10 . As such, the data show efficacious SARS‐CoV‐2 inactivation by all investigated methods, with >6.0 log 10 reduction. MPXV inactivation was also validated with all investigated methods with 6.9 log 10 reductions, although some commercial buffers required a longer incubation period to yield complete inactivation. These results are valuable for facilities, notably those without biosafety level‐3 capabilities, that need to implement rapid and reliable protocols common against both SARS‐CoV‐2 and MPXV.

show abstract

Section: Discussionsupporting

confidence: 92%

Rapid and reliable inactivation protocols for the diagnostics of emerging viruses: The example of SARS‐CoV‐2 and monkeypox virus

Quéromès

Bouscambert‐Duchamp

Oblette

et al. 2022

Journal of Medical Virology

View full text Add to dashboard Cite

show abstract

“… 47 Batéjat et al. 72 Biological samples Human sera samples from healthy donors Medical Center of Institut Pasteur, Paris, France NCT04325646 Human plasma samples from MPXV-infected patients Pitié-Salpêtrière hospital, Paris, France Palich et al. 43 Nouchi et al.…”

Section: Methodsmentioning

confidence: 99%

“… 47 Batéjat et al. 72 Experimental models: Cell lines Vero E6 ATCC Cat#CRL-1586; RRID:CVCL_0574 U2OS ATCC Cat#HTB-96; RRID:CVCL_0042 Software and algorithms Harmony High-Content Imaging and Analysis Software PerkinElmer Cat#HH17000012 Excel 365 Microsoft https://www.microsoft/com/en-ca/microsoft-365/excel Prism 9 Graphpad https://www.graphpad.com/ …”

Section: Methodsmentioning

confidence: 99%

Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals

Hubert

Guivel‐Benhassine

Bruel

et al. 2023

Cell Host & Microbe

Self Cite

View full text Add to dashboard Cite

“…Previous reports showed that unpurified SARS-CoV-2 in saliva samples can be detected with no RNA extraction step via a RT-LAMP assay [ 44 , 45 ]. As the reaction temperature of our LAMP assay is 65 °C and mpox virus can be inactivated at 60 °C in under 15 min [ 46 ], this LAMP assay can theoretically be used to detect mpox from crude clinical saliva samples without prior DNA extraction. Another limitation in this research is that we only used clinical DNA samples belonging to mpox Clade I to test our LAMP assay, but not those from Clade IIa and Clade IIb.…”

Section: Discussionmentioning

confidence: 99%

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

Zuo

Miao

et al. 2022

Viruses

View full text Add to dashboard Cite

A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the specific detection of mpox. The primer sets were designed to target regions in and around the N4R gene, and results showed a detection limit of 2 × 100 DNA copies, which is comparable to the gold-standard qPCR method currently used to detect mpox. Particularly, the assay provides results visible to the naked eye within 30 min. This test specifically detects mpox DNA with no cross-reactivity to related DNA viruses including Varicella Zoster Virus (VZV), Hepatitis B virus (HBV), Vaccinia virus (VACV), Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Human papillomavirus-16 (HPV-16) and Human papillomavirus-18 (HPV-18). Furthermore, the LAMP assay has been evaluated using clinical samples from laboratory-confirmed mpox patients and found to be consistent with the qPCR results. Our results show that this single-tube LAMP method can contribute to diagnosis of suspected mpox infections in the field and clinic, especially in regions with limited laboratory resources.

show abstract

Heat inactivation of monkeypox virus

Cited by 15 publications

References 7 publications

Rapid and reliable inactivation protocols for the diagnostics of emerging viruses: The example of SARS‐CoV‐2 and monkeypox virus

Rapid and reliable inactivation protocols for the diagnostics of emerging viruses: The example of SARS‐CoV‐2 and monkeypox virus

Complement-dependent mpox-virus-neutralizing antibodies in infected and vaccinated individuals

Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections

Contact Info

Product

Resources

About