Heat exposure induces oxidative stress and DNA damage in the male germ line†

Houston, Brendan J; Nixon, Brett; Martin, Jacinta H.; Iuliis, Geoffry N. De; Trigg, Natalie A.; Bromfield, Elizabeth G.; McEwan, Kristen E; Aitken, R. John

doi:10.1093/biolre/ioy009

Cited by 103 publications

(83 citation statements)

References 50 publications

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“…It is already known that exposure to elevated summer temperatures suppresses human seminal quality and that oxidative stress is a major mediator of this change [104,105]. Such exposure will be of particular significance to livestock species where exposure to elevated ambient temperatures might not only suppress fertility but also disrupt the true breeding of selected genetic traits [106].…”

Section: Lifestyle Exposuresmentioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

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This article addresses the importance of oxidative processes in both the generation of functional gametes and the aetiology of defective sperm function. Functionally, sperm capacitation is recognized as a redox-regulated process, wherein a low level of reactive oxygen species (ROS) generation is intimately involved in driving such events as the stimulation of tyrosine phosphorylation, the facilitation of cholesterol efflux and the promotion of cAMP generation. However, the continuous generation of ROS ultimately creates problems for spermatozoa because their unique physical architecture and unusual biochemical composition means that they are vulnerable to oxidative stress. As a consequence, they are heavily dependent on the antioxidant protection afforded by the fluids in the male and female reproductive tracts and, during the precarious process of insemination, seminal plasma. If this antioxidant protection should be compromised for any reason, then the spermatozoa experience pathological oxidative damage. In addition, situations may prevail that cause the spermatozoa to become exposed to high levels of ROS emanating either from other cells in the immediate vicinity (particularly neutrophils) or from the spermatozoa themselves. The environmental and lifestyle factors that promote ROS generation by the spermatozoa are reviewed in this article, as are the techniques that might be used in a diagnostic context to identify patients whose reproductive capacity is under oxidative threat. Understanding the strengths and weaknesses of ROS-monitoring methodologies is critical if we are to effectively identify those patients for whom treatment with antioxidants might be considered a rational management strategy.

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Section: Lifestyle Exposuresmentioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

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“…In reality, this list could be more extensive when cellular turnover is considered in the complexity of the whole organism. Systemic factors associated with inflammation, oxidative stress, metabolism, etc., which also have inputs on decisions of cellular proliferation and apoptosis during tissue turnover [1,3,10,61,[63][64][65][66][67]. Collectively, DNA damage greatly influences the ultimate fate of the cell.…”

Section: Tissue Renewal and Stem Cell Response To Dna Damagementioning

confidence: 99%

DNA damage and tissue repair: What we can learn from planaria

Barghouth

Thiruvalluvan

LeGro

et al. 2019

Seminars in Cell & Developmental Biology

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Faithful renewal of aging and damaged tissues is central to organismal lifespan. Stem cells (SCs) generate the cellular progeny that replenish adult tissues across the body but this task becomes increasingly compromised over time. The age related decline in SC-mediated tissue maintenance is a multifactorial event that commonly affects genome integrity. The presence of DNA damage in SCs that are under continuous demand to divide poses a great risk for age-related disorders such as cancer. However, performing analysis of SCs with genomic instability and the DNA damage response during tissue renewal present significant challenges. Here we introduce an alternative experimental system based on the planaria flatworm Schmidtea mediterranea to address at the organismal level studies intersecting SC-mediated tissue renewal in the presence of genomic instability. Planaria have abundant SCs (neoblasts) that maintain high rates of cellular turnover and a variety of molecular tools have been developed to induce DNA damage and dissect how neoblasts respond to this stressor. S. mediterranea displays high evolutionary conservation of DNA repair mechanisms and signaling pathways regulating adult SCs. We describe genetically induced-DNA damage models and highlight body-wide signals affecting cellular decisions such as survival, proliferation, and death in the presence of genomic instability. We also discuss transcriptomic changes in the DNA damage response during injury repair and propose DNA repair as key component of tissue regeneration. Additional studies using planaria will provide insights about mechanisms regulating survival and growth of cells with DNA damage during tissue renewal and regeneration.

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“…In this way, a number of spermatogonia available on the basement membrane may be affected. Also, the number of primary and secondary spermatocytes, spermatids and adult sperms reduced significantly (20). Oxidative stress can disrupt spermatogenesis in which the defective gametes with remodeled chromatin will be formed.…”

Section: Discussionmentioning

confidence: 99%

Effects of gallic acid on rat testopathy following morphine administration: an experimental study

Jalili

Roshankhah

Salahshoor

2020

J Herbmed Pharmacol

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Introduction: Morphine (MOR) as a psychoactive agent in the opium family causes free radicals accumulation which leads to failure in spermatogenesis. Gallic acid (GA), a polyphenolic acid, is found in various plants with antioxidant, anti-fungal, anti-viral, and anti-allergic activities. The purpose of this study was to evaluate the effects of GA against MOR-induced damage to the reproductive parameter of rats. Methods: Sixty-four male Wistar rats (8 weeks, 220-250 g) were categorized into 8 groups by random (n=8/each); normal control and MOR control groups; GA groups (5, 10, 20 mg/kg) and MOR + GA groups (5, 10, 20 mg/kg). Treatments were administered intraperitoneally (i.p), daily for 4 weeks. The sperm parameters, spermatogenesis index (SI), total antioxidant capacity, testosterone level, and seminiferous tube diameter (STD) were assessed. Results: All sperm parameters reduced significantly in the MOR control group than to the normal control group (P < 0.01). All parameters were significantly improved in GA and GA + MOR treatment groups compared to the MOR control group (P < 0.01). Conclusion: MOR caused a detrimental effect on male reproductive parameters. Also, no significant modifications were observed in all doses of GA treatments in comparison with the normal control group. GA compensates the toxic effect of MOR on reproductive parameters. Hence, GA administration is beneficial in MOR users.

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Heat exposure induces oxidative stress and DNA damage in the male germ line†

Cited by 103 publications

References 50 publications

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

DNA damage and tissue repair: What we can learn from planaria

Effects of gallic acid on rat testopathy following morphine administration: an experimental study

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