“Hearing” Bond Breakage. Measurement of Bond Rupture Forces Using a Quartz Crystal Microbalance

Dultsev, F. N.; Ostanin, and V. P.; Klenerman, David

doi:10.1021/la990789v

Cited by 68 publications

(45 citation statements)

References 12 publications

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“…Although it is unclear exactly how many antibody-antigen bonds were involved in the present spore-sensor surface binding, it suffices to say that the deduced force was consistent with the 1-200 pN range of antibody-antigen interactions. 18,20,28 While it is desirable to have more detailed information about the sporesensor surface binding strength, this is not within the scope of this study, but rather will be the focus of a future publication.…”

Section: Discussionmentioning

confidence: 99%

Label-free flow-enhanced specific detection of Bacillus anthracis using a piezoelectric microcantilever sensor

McGovern

Rest

Purohit

et al. 2008

Analyst

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Differentiation between species of similar biological structure is of critical importance in biosensing applications. Here, we report specific detection of Bacillus anthracis (BA) spores from that of close relatives, such as B. thuringiensis (BT), B. cereus (BC), and B. subtilis (BS) by varying the flow speed of the sampling liquid over the surface of a piezoelectric microcantilever sensor (PEMS). Spore binding to the anti-BA spore IgG coated PEMS surface is determined by monitoring the resonance frequency change in the sensor's impedance vs. frequency spectrum. Flow increases the resonance frequency shift at lower flow rates until the impingement force from the flow overcomes the binding strength of the antigen and decreases the resonance frequency shift at higher flow rates. We showed that the change from increasing to decreasing resonance frequency shift occurred at a lower fluid flow speed for BT, BC, and BS spores than for BA spores. This trend reduces the cross reactivity ratio of BC, BS, and BT to the anti-BA spore IgG immobilized PEMS from around 0.4 at low flow velocities to less than 0.05 at 3.8 mm s −1 . This cross reactivity ratio of 0.05 was essentially negligible considering the experimental uncertainty. The use of the same flow that is used for detection to further distinguish the specific binding (BA to anti-BA spore antibody) from nonspecific binding (BT, BC, and BS to anti-BA spore antibody) is unique and has great potential in the detection of general biological species.

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Section: Discussionmentioning

confidence: 99%

Label-free flow-enhanced specific detection of Bacillus anthracis using a piezoelectric microcantilever sensor

McGovern

Rest

Purohit

et al. 2008

Analyst

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“…6). The process, developed by Akubio (www.akubio.com), and termed rupture event scanning (REVS™) requires minimal sample preparation, works well in buffered solutions and in complex biological fluids such as serum, and takes only minutes to perform [96,98,99]. The magnitude of acoustic emission, or "loudness" of the sound emitted, is proportional to the number of particles present over at least six orders of magnitude, right down to the level of a single particle with a 839 Fig.…”

Section: Acoustic Biosensorsmentioning

confidence: 99%

Label-free screening of bio-molecular interactions

Cooper

2003

Analytical and Bioanalytical Chemistry

408

297

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The majority of techniques currently employed to interrogate a biomolecular interaction require some type of radio- or enzymatic- or fluorescent-labelling to report the binding event. However, there is an increasing awareness of novel techniques that do not require labelling of the ligand or the receptor, and that allow virtually any complex to be screened with minimal assay development. This review focuses on three major label-free screening platforms: surface plasmon resonance biosensors, acoustic biosensors, and calorimetric biosensors. Scientists in both academia and industry are using biosensors in areas that encompass almost all areas drug discovery, diagnostics, and the life sciences. The capabilities and advantages of each technique are compared and key applications involving small molecules, proteins, oligonucleotides, bacteriophage, viruses, bacteria, and cells are reviewed. The role of the interface between the biosensor surface (in the case of SPR and acoustic biosensors) and the chemical or biological systems to be studied is also covered with attention to the covalent and non-covalent coupling chemistries commonly employed.

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“…interfacial slippage and/or bond breaking can be detected by performing measurements on micronsized particles, whose larger inertial masses can more readily overcome the stronger frictional forces [33].…”

Section: Energy Dissipation In Adsorbed Films: Shakers and Moversmentioning

confidence: 99%

Surface science and the atomic-scale origins of friction: what once was old is new again

Krim

2002

Surface Science

117

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“Hearing” Bond Breakage. Measurement of Bond Rupture Forces Using a Quartz Crystal Microbalance

Cited by 68 publications

References 12 publications

Label-free flow-enhanced specific detection of Bacillus anthracis using a piezoelectric microcantilever sensor

Label-free flow-enhanced specific detection of Bacillus anthracis using a piezoelectric microcantilever sensor

Label-free screening of bio-molecular interactions

Surface science and the atomic-scale origins of friction: what once was old is new again

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