Hearing Ability of Prairie Voles (<i>Microtus ochrogaster</i>)

New, Emily M.; Li, Ben‐Zheng; Lei, Tim C.; McCullagh, Elizabeth A.

doi:10.1101/2021.10.07.463519

Cited by 2 publications

(4 citation statements)

References 31 publications

(47 reference statements)

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“…Auditory brain stem response recordings were performed using similar methods from previously published study ( Benichoux et al, 2018 ; McCullagh et al, 2020a ; New et al, 2021 ). Animals were anesthetized using two mixtures of ketamine-xylazine 60 mg/kg ketamine and 10 mg/kg xylazine for initial induction followed by maintenance doses of 25 mg/kg ketamine and 12 mg/kg xylazine.…”

Section: Methodsmentioning

confidence: 99%

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Auditory Brain Stem Responses in the C57BL/6J Fragile X Syndrome-Knockout Mouse Model

Chawla

McCullagh

2022

Front. Integr. Neurosci.

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Sensory hypersensitivity, especially in the auditory system, is a common symptom in Fragile X syndrome (FXS), the most common monogenic form of intellectual disability. However, linking phenotypes across genetic background strains of mouse models has been a challenge and could underly some of the issues with translatability of drug studies to the human condition. This study is the first to characterize the auditory brain stem response (ABR), a minimally invasive physiological readout of early auditory processing that is also used in humans, in a commonly used mouse background strain model of FXS, C57BL/6J. We measured morphological features of pinna and head and used ABR to measure the hearing range, and monaural and binaural auditory responses in hemizygous males, homozygous females, and heterozygous females compared with those in wild-type mice. Consistent with previous study, we showed no difference in morphological parameters across genotypes or sexes. There was no significant difference in hearing range between the sexes or genotypes, however there was a trend towards high frequency hearing loss in male FXS mice. In contrast, female mice with homozygous FXS had a decreased amplitude of wave IV of the monaural ABR, while there was no difference in males for amplitudes and no change in latency of ABR waveforms across sexes and genotypes. Finally, males with FXS had an increased latency of the binaural interaction component (BIC) at 0 interaural timing difference compared with that in wild-type males. These findings further clarify auditory brain stem processing in FXS by adding more information across genetic background strains allowing for a better understanding of shared phenotypes.

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Section: Methodsmentioning

confidence: 99%

“…The custom python software was used to analyze evoked potentials for monaural and binaural stimuli ( New et al, 2021 ). To account for fluctuation in the baseline signal of the ABR, raw traces were zeroed to establish a baseline across traces.…”

Section: Methodsmentioning

confidence: 99%

Auditory Brain Stem Responses in the C57BL/6J Fragile X Syndrome-Knockout Mouse Model

Chawla

McCullagh

2022

Front. Integr. Neurosci.

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show abstract

“…ABR recordings were performed using similar methods to previously published work (Benichoux et al, 2018;McCullagh et al, 2020a;New et al, 2021). Animals were anesthetized using two mixtures of ketamine-xylazine 60mg/kg ketamine and 10mg/kg xylazine for initial induction followed by maintenance doses of 25 mg/kg ketamine and 12 mg/kg xylazine.…”

Section: Abrsmentioning

confidence: 99%

“…Custom python software was used to analyze evoked potentials for monaural and binaural stimuli (New et al, 2021). To account for fluctuation in the baseline signal of the ABR, raw traces were zeroed to establish a baseline across traces.…”

Section: Analysis Of Abr Waveformsmentioning

confidence: 99%

Auditory Brainstem Responses in the C57BL/6J Fragile X Syndrome Knockout Mouse Model

Chawla

McCullagh

2021

Preprint

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Sensory hypersensitivity, especially in the auditory system, is a common symptom in Fragile X Syndrome (FXS), the most common monogenic form of intellectual disability. However, linking phenotypes across genetic background strains of mouse models has been a challenge and could underly some of the issues with translatability of drug studies to the human condition. This study is the first to characterize the auditory brainstem response (ABR), a minimally invasive physiological readout of early auditory processing that is also used in humans, in a commonly used mouse background strain model of FXS, C57BL/6J. We measured morphological features of pinna and head and used ABR to measure hearing range, monaural and binaural auditory responses in hemizygous males, homozygous females and heterozygous females compared to wildtype mice. Consistent with previous work we showed no difference in morphological parameters across genotypes or sexes. Male FXS mice had increased threshold for high frequency hearing at 64 kHz compared to wildtype males, while females had no difference in hearing range between genotypes. In contrast, female homozygous FXS mice had decreased amplitude of wave IV of the monaural ABR, while there was no difference in males for amplitudes and no change in latency of ABR waveforms across sexes and genotypes. Lastly, FXS males had increased latency of the binaural interaction component (BIC) at 0 ITD compared to wildtype males. These findings further clarify auditory brainstem processing in FXS by adding more information across genetic background strains allowing for a better understanding of shared phenotypes.

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Hearing Ability of Prairie Voles (Microtus ochrogaster)

Cited by 2 publications

References 31 publications

Auditory Brain Stem Responses in the C57BL/6J Fragile X Syndrome-Knockout Mouse Model

Auditory Brain Stem Responses in the C57BL/6J Fragile X Syndrome-Knockout Mouse Model

Auditory Brainstem Responses in the C57BL/6J Fragile X Syndrome Knockout Mouse Model

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