Healthy Twin: A Twin-Family Study of Korea — Protocols and Current Status

Sung, Joohon; Cho, Sung-Il; Lee, Kayoung; Ha, Mina; Choi, Eun-Young; Choi, Jaewon; Kim, Ho; Kim, Ji-Hae; Hong, Kyoung Sue; Kim, Yeon-Ju; Yoo, Keun-Young; Park, Chan; Song, Yun-Mi

doi:10.1375/twin.9.6.844

Cited by 108 publications

(31 citation statements)

References 15 publications

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“…Study subjects and collection of fecal samples Human stool samples were collected from patients in two hospitals participating in the Korean healthy twin cohort study in Seoul and Busan, South Korea, as described previously (Sung et al, 2006). Human subjects aged 30-60 years (58.4 ± 14.9) with a BMI in the normal range (24.8 ± 4.0 kg m À 2 ) and who had not taken any antibiotics for at least 1 month prior to sample collection were included in the study.…”

Section: Methodsmentioning

confidence: 99%

Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota

Lee

Cantarel²,

Henrissat

et al. 2013

The ISME Journal

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Glycoside hydrolases (GHs), the enzymes that breakdown complex carbohydrates, are a highly diversified class of key enzymes associated with the gut microbiota and its metabolic functions. To learn more about the diversity of GHs and their potential role in a variety of gut microbiomes, we used a combination of 16S, metagenomic and targeted amplicon sequencing data to study one of these enzyme families in detail. Specifically, we employed a functional gene-targeted metagenomic approach to the 1-4-a-glucan-branching enzyme (gBE) gene in the gut microbiomes of four host species (human, chicken, cow and pig). The characteristics of operational taxonomic units (OTUs) and operational glucan-branching units (OGBUs) were distinctive in each of hosts. Human and pig were most similar in OTUs profiles while maintaining distinct OGBU profiles. Interestingly, the phylogenetic profiles identified from 16S and gBE gene sequences differed, suggesting the presence of different gBE genes in the same OTU across different vertebrate hosts. Our data suggest that gene-targeted metagenomic analysis is useful for an in-depth understanding of the diversity of a particular gene of interest. Specific carbohydrate metabolic genes appear to be carried by distinct OTUs in different individual hosts and among different vertebrate species' microbiomes, the characteristics of which differ according to host genetic background and/or diet.

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Section: Methodsmentioning

confidence: 99%

Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota

Lee

Cantarel²,

Henrissat

et al. 2013

The ISME Journal

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show abstract

“…Participants were not ascertained by their health status or psychiatric disorders. The Healthy Twin study is an ongoing nationwide community study and details about the overall methodology have been previously published (Sung et al, 2006). Between April 2005 and December 2007, a total of 2,278 Korean adult (age range 30-79 years of age) same-sexed twins and their first-degree adult family members were recruited.…”

Section: Subjects Sampling and Study Designmentioning

confidence: 99%

Relationships Between State and Trait Anxiety Inventory and Alcohol Use Disorder Identification Test Scores Among Korean Twins and Families: The Healthy Twin Study

Sung

Lee²,

Song

et al. 2011

Twin Res Hum Genet

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We explored heritabilities of the State and Trait Anxiety Inventory (STAI) and the Alcohol Use Disorders Identification Test (AUDIT), and associations including genetic and environmental correlations between the phenotypes among Korean twins and their families. We analyzed the data of 1,748 participants (835 men, 913 women, 656 individuals of monozygotic twins, 173 individuals of same-sexed dizygotic twins, 919 non-twin family members, age 30–79 years) from the Healthy Twin study. Heritabilities and bivariate analyses were assessed using the SOLAR package software. In the methods of generalized estimation equations, women in the 4th quartile of state and trait scores were 17% and 15%, respectively more likely to be hazardous alcohol users compared to women in the lower three quartiles (P< .05). However, there were no significant associations between these phenotypes in men. After adjusting for age and squared age, the heritability estimates were 0.26 in men and 0.34 in women for the state score; for the trait score, 0.35 in men and 0.31 in women; for the AUDIT score, 0.32 in men and 0.37 in women (P< .001). After adjusting for age and squared age, there was a significant genetic correlation between the trait score and the AUDIT score, and a significant non-genetic correlation between the state score and the AUDIT score in women, while there were no significant genetic or non-genetic correlations between these phenotypes in men. The STAI and AUDIT scores are heritable in Koreans and the relationships between these phenotypes may be inconsistent by sex.

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“…The study subjects were participants in the Healthy Twin Study, which has been a part of the Korean Genomic Epidemiologic Study since 2005 (Sung et al, 2006). The zygosity of twins was determined by a questionnaire, resulting in a greater than 90% accuracy, and genetic analysis using 16 short tandem repeat markers (15 autosomal-markers and one sex-determining marker; AmpFlSTR Identifier Kit; Perkin Elmer, Waltham, MA, USA; Song et al, 2010).…”

Section: Subjects and Study Designmentioning

confidence: 99%

Changes in Weight and Cardiovascular Disease Risk Factors in Monozygotic Twins: The Healthy Twin Study

2015

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We aimed to assess the non-genetic contribution to the associations between the change in weight and changes in cardiovascular disease (CVD) risk factors. This analysis included 194 Korean monozygotic (MZ) twin pairs (116 men, 272 women; mean age, 38.5 ± 6.8 years) who were first examined for weight and CVD risk factors (blood pressure (BP), glucose, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL), and low-density lipoprotein cholesterol (LDL)) between December 2005 and December 2008, and returned for a repeat examination after 2.7 ± 0.9 years. The within-pair correlations were 0.21 for the change in weight and 0.05-0.42 for the changes in CVD risk factors. Bivariate analyses showed significant environmental correlations shared between the change in weight and the changes in CVD risk factors (p < .05), except for glucose, while there were no significant genetic effects shared between the phenotypes. After adjusting for baseline values of weight, smoking, and alcohol consumption, diastolic blood pressure (DBP), TG, TC, and LDL significantly increased by 1.6 mmHg, 0.09 mmol/L, 0.10 mmol/L, and 0.09 mmol/L, respectively, per 1 kg increase in within-pair differences in weight change. In Korean MZ twins, similarity between twins for changes in weight and CVD risk factors were small to moderate, and non-genetic factors were responsible for the associations between the change in weight and changes in DBP, TG, TC, and LDL.

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Healthy Twin: A Twin-Family Study of Korea — Protocols and Current Status

Cited by 108 publications

References 15 publications

Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota

Gene-targeted metagenomic analysis of glucan-branching enzyme gene profiles among human and animal fecal microbiota

Relationships Between State and Trait Anxiety Inventory and Alcohol Use Disorder Identification Test Scores Among Korean Twins and Families: The Healthy Twin Study

Changes in Weight and Cardiovascular Disease Risk Factors in Monozygotic Twins: The Healthy Twin Study

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