Healthy Subjects Differentially Respond to Dietary Capsaicin Correlating with Specific Gut Enterotypes

Kang, Chao; Zhang, Yong; Zhu, Xiaohui; Li, Kai; Wang, Xiaolan; Chen, Mengting; Wang, Jian; Chen, Hua; Hui, Suocheng; Huang, Li; Zhang, Qianyong; Zhu, Jingjing; Wang, Bin; Mi, Mantian

doi:10.1210/jc.2016-2786

Cited by 115 publications

(116 citation statements)

References 40 publications

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“…Interestingly, our recent study has shown similar results with CAP intervention in human subjects (16). This would indicate the possibility that lower abundance of Gram-negative microbiota must be responsible for the low abundance of KEGG orthology (KO) belonging to the LPS biosynthesis pathway in the HFD+C group.…”

Section: Discussionsupporting

confidence: 74%

“…2D and E and G and H). Interestingly, our previous study also found that the levels of butyrogenic bacteria are elevated in humans after 2 weeks of CAP intervention (16). Bacterial butyrate production is a key function attributed to a “healthy” gut microbiota because of the preferred use of this SCFA as an energy source for intestinal epithelial cells (29).…”

Section: Discussionmentioning

confidence: 83%

“…Capsaicin (CAP) is the major pungent component in red chili (genus Capsicum ) that provides flavor to food without increasing the number of calories (15). Growing evidence indicates that CAP could improve obesity and related comorbidities, suggesting that it could be a new promising therapeutic strategy (16). Previous studies have demonstrated that dietary CAP can reduce HFD-induced increase in body weight and glucose metabolism abnormalities (17–19).…”

Section: Introductionmentioning

confidence: 99%

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Gut Microbiota Mediates the Protective Effects of Dietary Capsaicin against Chronic Low-Grade Inflammation and Associated Obesity Induced by High-Fat Diet

Kang

Wang

Kaliannan

et al. 2017

mBio

Self Cite

196

192

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Metabolic endotoxemia originating from dysbiotic gut microbiota has been identified as a primary mediator for triggering the chronic low-grade inflammation (CLGI) responsible for the development of obesity. Capsaicin (CAP) is the major pungent bioactivator in chili peppers and has potent anti-obesity functions, yet the mechanisms linking this effect to gut microbiota remain obscure. Here we show that mice fed a high-fat diet (HFD) supplemented with CAP exhibit lower levels of metabolic endotoxemia and CLGI associated with lower body weight gain. High-resolution responses of the microbiota were examined by 16S rRNA sequencing, short-chain fatty acid (SCFA) measurements, and phylogenetic reconstruction of unobserved states (PICRUSt) analysis. The results showed, among others, that dietary CAP induced increased levels of butyrate-producing Ruminococcaceae and Lachnospiraceae, while it caused lower levels of members of the lipopolysaccharide (LPS)-producing family S24_7. Predicted function analysis (PICRUSt) showed depletion of genes involved in bacterial LPS synthesis in response to CAP. We further identified that inhibition of cannabinoid receptor type 1 (CB1) by CAP also contributes to prevention of HFD-induced gut barrier dysfunction. Importantly, fecal microbiota transplantation experiments conducted in germfree mice demonstrated that dietary CAP-induced protection against HFD-induced obesity is transferrable. Moreover, microbiota depletion by a cocktail of antibiotics was sufficient to block the CAP-induced protective phenotype against obesity, further suggesting the role of microbiota in this context. Together, our findings uncover an interaction between dietary CAP and gut microbiota as a novel mechanism for the anti-obesity effect of CAP acting through prevention of microbial dysbiosis, gut barrier dysfunction, and chronic low-grade inflammation.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gut Microbiota Mediates the Protective Effects of Dietary Capsaicin against Chronic Low-Grade Inflammation and Associated Obesity Induced by High-Fat Diet

Kang

Wang

Kaliannan

et al. 2017

mBio

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196

192

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“…SCFAs were measured by gas chromatography from caecum contents as described previously [40]. A 1:5 dilution of caecum in 1 M HCl was centrifuged (12000 × g , 15 min), and diethylacetic acid (Sigma) was added to a final concentration of 1 mM as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus casei Zhang and vitamin K2 prevent intestinal tumorigenesis in mice via adiponectin-elevated different signaling pathways

Zhang

Zhao

et al. 2017

Oncotarget

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The incidence of colon cancer has increased considerably and the intestinal microbiota participate in the development of colon cancer. We showed that the L. casei Zhang or vitamin K2 (Menaquinone-7) intervention significantly alleviated intestinal tumor burden in mice. This was associated with increased serum adiponectin levels in both treatments. But osteocalcin level was only increased by L. casei Zhang. Furthermore, the anti-carcinogenic actions of L. casei Zhang were mediated by hepatic Chloride channel-3(CLCN3)/Nuclear Factor Kappa B(NF-κB) and intestinal Claudin15/Chloride intracellular channel 4(CLIC4)/Transforming Growth Factor Beta(TGF-β) signaling, while the vitamin K2 effect involved a hepatic Vitamin D Receptor(VDR)-phosphorylated AMPK signaling pathway. Fecal DNA sequencing by the Pacbio RSII method revealed there was significantly lower Helicobacter apodemus, Helicobacter mesocricetorum, Allobaculum stercoricanis and Adlercreutzia equolifaciens following both interventions compared to the model group. Moreover, different caecum acetic acid and butyric acid levels and enrichment of other specific microbes also determined the activity of the different regulatory pathways. Together these data show that L. casei Zhang and Vitamin K2 can suppress gut risk microbes and promote beneficial microbial metabolites to reduce colonic tumor development in mice.

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“…Using these enterotypes, microbiome-based nutritional or drug metabolism can be estimated and clinically useful to prevent idiosyncratic hypersensitivity to food and drugs, and metabolic activation-induced toxicity [3]. Moreover, nutritional recommendation could be individually selected based on personal enterotypes to control the metabolism-linked disorders such as hypercholesterolemia, diabetes and obesity [4]. …”

Section: Features Of Stability Diversity Functional Redundancy Andmentioning

confidence: 99%

Microbiome-Linked Crosstalk in the Gastrointestinal Exposome towards Host Health and Disease

Moon

2016

Pediatr Gastroenterol Hepatol Nutr

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The gastrointestinal exposome represents the integration of all xenobiotic components and host-derived endogenous components affecting the host health, disease progression and ultimately clinical outcomes during the lifespan. The human gut microbiome as a dynamic exposome of commensalism continuously interacts with other exogenous exposome as well as host sentineling components including the immune and neuroendocrine circuit. The composition and diversity of the microbiome are established on the basis of the luminal environment (physical, chemical and biological exposome) and host surveillance at each part of the gastrointestinal lining. Whereas the chemical exposome derived from nutrients and other xenobiotics can influence the dynamics of microbiome community (the stability, diversity, or resilience), the microbiomes reciprocally alter the bioavailability and activities of the chemical exposome in the mucosa. In particular, xenobiotic metabolites by the gut microbial enzymes can be either beneficial or detrimental to the host health although xenobiotics can alter the composition and diversity of the gut microbiome. The integration of the mucosal crosstalk in the exposome determines the fate of microbiome community and host response to the etiologic factors of disease. Therefore, the network between microbiome and other mucosal exposome would provide new insights into the clinical intervention against the mucosal or systemic disorders via regulation of the gut-associated immunological, metabolic, or neuroendocrine system.

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Healthy Subjects Differentially Respond to Dietary Capsaicin Correlating with Specific Gut Enterotypes

Abstract: Our study showed that gut enterotypes may influence the beneficial effects of dietary CAP, providing new evidence for the personalized nutrition guidance of CAP intervention on health promotion linking with gut microbiota patterns.

Cited by 115 publications

References 40 publications

Gut Microbiota Mediates the Protective Effects of Dietary Capsaicin against Chronic Low-Grade Inflammation and Associated Obesity Induced by High-Fat Diet

Gut Microbiota Mediates the Protective Effects of Dietary Capsaicin against Chronic Low-Grade Inflammation and Associated Obesity Induced by High-Fat Diet

Lactobacillus casei Zhang and vitamin K2 prevent intestinal tumorigenesis in mice via adiponectin-elevated different signaling pathways

Microbiome-Linked Crosstalk in the Gastrointestinal Exposome towards Host Health and Disease

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