Healthy scents: microbial volatiles as new frontier in antibiotic research?

Ávalos, Mariana; Wezel, Gilles P. van; Raaijmakers, Jos M.; Garbeva, Paolina

doi:10.1016/j.mib.2018.02.011

Cited by 56 publications

(44 citation statements)

References 75 publications

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“…Monoterpenes contribute to the antibiotic activity in microbial volatiles [25]. In this study, artificial γ-terpinene, α-terpinene, (-)-4-terpineol, and their mixtures showed antifungal effect against tested pathogens (Tables 3-5 and 7).…”

Section: Discussionmentioning

confidence: 58%

An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

et al. 2019

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Volatile organic compounds (VOCs) from endophytic fungi are becoming a potential antibiotic resource. The inhibitive effects of VOCs produced by an endophytic fungus in Leucaena leucocephala were investigated on plant pathogens in this study. Using standard morphological methods and multigene phylogeny, the fungus was identified as Diaporthe apiculatum strain FPYF 3052. Utilizing a two- compartment Petri plate bioassay method, the VOCs from this fungus showed bioactivity ranging from 23.8% to 66.7% inhibition on eight plant pathogens within 24 hours. The SPME-GC/MS technique identified fifteen volatile compounds with dominant terpenoids γ-terpinene (39.8%), α-terpinene (17.2%), and (-)-4-terpineol (8.4%) from the VOCs. Commercial α-terpinene, γ-terpinene, and (-)-4-terpineol demonstrated inhibition on the tested pathogens at concentrations from 0.2 to 1.0 µl/ml within 72 h in the bioassay system. The inhibition rates were from 28% to 100% percent using 1.0 µl/ml within 48 h. (-)-4-Terpineol was the most active of the terpenoids causing up to 100% inhibition. The data illustrate that these monoterpenes play an important role in the inhibitive bioactivity of the VOCs of D. apiculatum FPYF 3052. Most importantly, (-)-4-terpineol is now for the first time, reported to have capability of strong antifungal activity and could be developed as an antibiotic substance.

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Section: Discussionmentioning

confidence: 58%

An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

et al. 2019

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“…Interestingly, although the ability of microorganisms to produce structurally diverse volatile compounds has been known for decades [16], their antimicrobial activities have only recently attracted attention making them potential candidates for future drug development (reviewed in [17]). In addition, volatiles can have synergistic effects with soluble antimicrobials.…”

Section: Microbial Chemical Diversitymentioning

confidence: 99%

Microbe-driven chemical ecology: past, present and future

et al. 2019

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Recent developments in microbiome biology and chemical analytics have revealed the relevance of microbial chemical communication and its networks for microbial ecology. Deciphering chemical interactions, however, is challenging and our understanding of Microbial Chemical Ecology (MCE) under natural conditions still remains fragmented. Here, we aim to summarize what is currently known in the field of MCE. We highlight new tools and methodological challenges and discuss future perspectives of this emerging field. We describe the factors affecting the production and environmental transport of signalling molecules, evaluate their metabolic and ecological functions, and discuss approaches to address future challenges in MCE. Our summary commends that future developments in the field of MCE will need to include studies involving organisms of all levels, and consider mechanisms underlying the communication including viruses, micro and macro-organisms in their natural environments. 3 Background Chemical ecology first appeared as a keystone discipline in the early 1950's, advancing our understanding of insect communication and plant chemical defenses [1]. However, chemical communication is not restricted to plant-insect and plant-plant interactions. In fact, chemically mediated relationships are now being recognized as common in the microbial world across terrestrial and aquatic ecosystems (Figure 1). Bonnie Bassler is one of the pioneers of microbial chemical communication being amongst the first to discover bacterial intra-specific quorum sensing via autoinducing chemical compounds. This mechanism is now proving to play a fundamental role in both intraspecific and interspecific interactions [2, 3]. Prof. Bassler coined the term "microbial language" and it was her initial work and the numerous follow-up studies that brought chemical communication between microbes into the spotlight. Researchers in the field of microbial ecology are recognizing the important roles that chemical communication and interactions play across all ecosystems (reviewed in [4]). In fact, the oldest form of communication is probably the chemical communication between microorganisms and only later evolved in plants, insects and other higher organisms [5]. Thus, by deciphering the chemical language, we will be able to better understand how species interact in their ecosystems. However, understanding the theoretical foundations of chemical language (its origin and diversity) is challenging and has been rarely studied. Until now, the topic of Microbial Chemical Ecology (MCE) has been largely neglected by microbiologists. The reason stems from methodological constraints concerning the analysis of microbiological communities under natural conditions. Furthermore, most of the research for natural products is focused on chemical and biochemical approaches and drug discovery with a less of an emphasis on ecological aspects. The traditional separation of disciplines limits our

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“…The NOMETA-inducedm etabolites were detected in 45.5 %( 5/11), 18.2 %( 2/11), and 18.2 %o ft ested strains (in total 63.6 %, 7/11) treated by sodiumn itroprusside, an NO donor,a nd 1% NO in N 2 gas and cyclo-(l-Phe-trans-4hydroxy-l-Pro), respectively.A lthough the precise mechanism of NOMETAr emains elusive, this findings uggestst hat the acti- vation of cryptic genes of SMs mediated by gas signal molecules may have long been neglected.A dditionally,t he release of membrane-permeable volatile molecules may have important impacts on microbial interactions in environmental communities. [22] 3. Inter-kingdom Physical Contact-induced Chromatin Remodeling…”

Section: Diffusible Molecule-mediated Chemical Attack and Defensementioning

confidence: 99%

Cryptic Chemical Communication: Secondary Metabolic Responses Revealed by Microbial Co‐culture

Liu

Kakeya

2020

Chemistry An Asian Journal

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Microbial secondary metabolites (SMs) have long been viewed as as ignificants ource of novel pharmaceutical and agrochemical molecules. With the increasing availability of genomic data, numerous biosynthetic gene clusters (BGCs) have been discovered. Despite the presence of tens of thousands of BGCs that can theoretically produce extremely diverse SMs, many gene clusters remain in as ilent state under axenic culture conditions. Co-culture is ap romising research approach as it stimulates the expression of cryptic BGCs to produce novel metabolites and also mimics naturali nterspeciesi nteractions in al aboratory environment. In recenty ears, the roles of SMs in microbial communication have caught the attention of researchers and our understanding of microbes and their productiono fr emarkable SMs has improved. SMs may be extensively involved in av ariety of communication eventsa mong microorganisms. We herein summarize certainr epresentative findings in the field of chemical communication involving SMs in co-culture systems.

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Healthy scents: microbial volatiles as new frontier in antibiotic research?

Cited by 56 publications

References 75 publications

An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

Microbe-driven chemical ecology: past, present and future

Cryptic Chemical Communication: Secondary Metabolic Responses Revealed by Microbial Co‐culture

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