Healthy Functional Food Properties of Phenolic Compounds Isolated from Ulmus pumila

Kim, Kyung-Bum; Jo, Bun-Sung; Park, Hye-Jin; Park, Ki-Tae; An, Bong-Jeun; Ahn, Dong-Hyun; Kim, Myung-Uk; Chae, Jung-Woo; Cho, Young-Je

doi:10.11002/kjfp.2012.19.6.909

Cited by 18 publications

(5 citation statements)

References 13 publications

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“…Ulmus pumila L., a deciduous tree from the family Ulmaceae, is widely distributed in China, Korea, and Japan (Lee et al, 2018). U. pumila has a long history of cultivation, and it has been used as a lifesaving tree during famine due to the edible and medicinal properties of its leaves, roots, bark, and fruits (Kim et al, 2012). Young U. pumila leaf is rich in starch and protein (Sun, 2014), and its edible parts have been used in noodle‐making to improve their tensile properties (Liu & Wang, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Condensed tannins from Ulmus pumila L. leaves induce G2 /M phase arrest and apoptosis via caspase‐cascade activation in TFK ‐1 cholangiocarcinoma cells

Ren

Qin

Wang

et al. 2022

Journal of Food Biochemistry

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Condensed tannins the polyphenolic compounds that are widespread in plants have been proved to have antitumor potential. Here, we purified the bioactive condensed tannins from leaves of Ulmus pumila L. and explored their structural characteristics, antitumor effect on TFK-1 cholangiocarcinoma cells as well as the related potential mechanism. The UV-Vis, FT-IR spectroscopy, ESI-Full-MS, and thiolysis-HPLC-ESI-MS demonstrated that U. pumila condensed tannins (UCTs) consisted essentially of procyanidins with epicatechin as the main flavan-3-ol extension unit. The UCTs could significantly reduce the survival rate of human cholangiocarcinoma TFK-1, SK-CHA-1, and MZ-CHA-1 cells with the better inhibitory effect on TFK-1 cell proliferation. Flow cytometric assay showed that UCTs affected TFK-1 survival by G2/M phase arrest and inducing apoptosis in a dose-dependent manner. In addition, a total of 6592 differentially expressed genes (DEGs), consisting of 94 upregulated and 6498 downregulated DEGs, were identified between untreated and UCTs-treated TFK-1 cells using RNA-seq technology. Enrichment analysis based on the KEGG database revealed that these DEGs were closely associated with cell cycle and p53 apoptotic signaling pathways. Furthermore, qRT-PCR confirmed that treatment of UCTs to TFK-1 cells caused significant changes in the expression of cyclin E, cdc25 A, cytochrome c, caspase-3, and caspase-8. These results indicated that UCTs exhibited the growth inhibition effect on TFK-1 cells possibly via G2/M cell cycle arrest and activation of caspasecascade to induce apoptosis, and had potential as an anti-cholangiocarcinoma drug for further development. Practical applicationsUlmus pumila L. as a valuable tree species has been widely used in fields of medicine and food. Condensed tannins, the polyphenolic compounds widespread in plants, have been proved to have antitumor potential and be safe to normal cells. In this study, the condensed tannins from leaves of U. pumila (UCTs) remarkably suppressed cholangiocarcinoma (CCA) cell viability possibly via G2/M cell cycle arrest and activation of

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Section: Introductionmentioning

confidence: 99%

“…U. pumila has a long history of cultivation, and it has been used as a lifesaving tree during famine due to the edible and medicinal properties of its leaves, roots, bark, and fruits (Kim et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Condensed tannins from Ulmus pumila L. leaves induce G2 /M phase arrest and apoptosis via caspase‐cascade activation in TFK ‐1 cholangiocarcinoma cells

Ren

Qin

Wang

et al. 2022

Journal of Food Biochemistry

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“…These properties make it suitable for ship building, architecture, furniture, decoration, and handicrafts [ 5 , 6 , 7 , 8 ]. U. pumila has a long history of cultivation, and it has been used as a life-saving tree during famine due to the edible and medicinal properties of its bark, roots, leaves, and fruits [ 9 , 10 ]. The bark is rich in sterols and starch, with up to 16.14% fiber and 17% polysaccharides, and is used for food, paper, and other products [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Gene Coexpression Network Analysis Indicates that Hub Genes Related to Photosynthesis and Starch Synthesis Modulate Salt Stress Tolerance in Ulmus pumila

Chen

Liu

Zhang

et al. 2021

IJMS

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Ulmus pumila L. is an excellent afforestation and biofuel tree that produces high-quality wood, rich in starch. In addition, U. pumila is highly adaptable to adverse environmental conditions, which is conducive to its utilization for vegetating saline soils. However, little is known about the physiological responses and transcriptional regulatory network of U. pumila under salt stress. In this study, we exposed five main cultivars in saline–alkali land (Upu2, 5, 8, 11, and 12) to NaCl stress. Of the five cultivars assessed, Upu11 exhibited the highest salt resistance. Growth and biomass accumulation in Upu11 were promoted under low salt concentrations (<150 mM). However, after 3 months of continuous treatment with 150 mM NaCl, growth was inhibited, and photosynthesis declined. A transcriptome analysis conducted after 3 months of treatment detected 7009 differentially expressed unigenes (DEGs). The gene annotation indicated that these DEGs were mainly related to photosynthesis and carbon metabolism. Furthermore, PHOTOSYNTHETIC ELECTRON TRANSFERH (UpPETH), an important electron transporter in the photosynthetic electron transport chain, and UpWAXY, a key gene controlling amylose synthesis in the starch synthesis pathway, were identified as hub genes in the gene coexpression network. We identified 25 and 62 unigenes that may interact with PETH and WAXY, respectively. Overexpression of UpPETH and UpWAXY significantly increased the survival rates, net photosynthetic rates, biomass, and starch content of transgenic Arabidopsis plants under salt stress. Our findings clarify the physiological and transcriptional regulators that promote or inhibit growth under environmental stress. The identification of salt-responsive hub genes directly responsible for photosynthesis and starch synthesis or metabolism will provide targets for future genetic improvements.

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“…Ulmus macrocarpa Hance has been used as an oriental medicinal plant for decades as a traditional treatment for gastric ulcers, gastritis, bacterial infections, edema and infiammation in South Korea [10,11]. However, a few studies have evaluated that U .…”

Section: Introductionmentioning

confidence: 99%

Ulmus macrocarpa Hance modulates lipid metabolism in hyperlipidemia via activation of AMPK pathway

et al. 2019

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Ulmus macrocarpa Hance as an oriental medicinal plant has shown enormous potential for the treatment of several metabolic disorders in Korea. Hyperlipidemia, which is characterized by the excess accumulation of lipid contents in the bloodstream, may lead to several cardiovascular diseases. Therefore, in this study, anti-hyperlipidemic potential of U . macrocarpa water extract (UME) was examined in vitro and in vivo using HepG2 cells and experimental rats, respectively. The hyperlipidemia in experimental rats was induced by the high-cholesterol diet (HCD) followed by oral administration of various concentrations (25, 50 and 100 mg/kg) of UME for 6 weeks. As a result, the UME significantly improved the biochemical parameters such as increased the level of triglyceride, total cholesterol, and low-density lipoprotein cholesterol as well as reduced the high-density lipoprotein cholesterol in the HCD-fed rats. In addition, UME also prevented lipid accumulation through regulating AMPK activity and lipid metabolism proteins (ACC, SREBP1 and HMGCR) in the HCD-fed rats as compared to the controls. Moreover, similar pattern of gene expression levels was confirmed in oleic acid (OA)-treated HepG2 cells. Taken together, our results indicate that UME prevents hyperlipidemia via activating the AMPK pathway and regulates lipid metabolism. Thus, based on the above findings, it is estimated that UME could be a potential therapeutic agent for preventing the hyperlipidemia.

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Healthy Functional Food Properties of Phenolic Compounds Isolated from Ulmus pumila

Cited by 18 publications

References 13 publications

Condensed tannins from Ulmus pumila L. leaves induce G2 /M phase arrest and apoptosis via caspase‐cascade activation in TFK ‐1 cholangiocarcinoma cells

Condensed tannins from Ulmus pumila L. leaves induce G2 /M phase arrest and apoptosis via caspase‐cascade activation in TFK ‐1 cholangiocarcinoma cells

Gene Coexpression Network Analysis Indicates that Hub Genes Related to Photosynthesis and Starch Synthesis Modulate Salt Stress Tolerance in Ulmus pumila

Ulmus macrocarpa Hance modulates lipid metabolism in hyperlipidemia via activation of AMPK pathway

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