Healthy aging and muscle function are positively associated with NAD+ abundance in humans

Abstract

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Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

“…The protocol below describes the procedure for polar metabolomics in human muscle biopsies ( Janssens et al., 2022 ), but the same protocol has been successfully applied for human ( Remie et al., 2021 ; Connell et al., 2021 ; Remie et al., 2020 ), mouse ( van der Stelt et al., 2021 ; Zapata-Pérez et al., 2021 ) or rat tissues (brown adipose tissue, white adipose tissue, heart, liver, kidney, brain, placenta, arterial plaque), cultured cells (primary skin fibroblasts, Human Embryonic Kidney (HEK) cells, bronchial epithelial cells, cumulus cells, oocytes, organoids), blood samples ( Zapata-Pérez et al., 2021 ) (serum, plasma, erythrocytes, platelets, peripheral blood mononuclear cells, whole blood, macrophages), other bodily fluids (saliva, sweat, urine, fecal water, bile, cerebrospinal fluid), model organisms ( Molenaars et al., 2021 ; Liu et al., 2020 ; Gao et al., 2018 ; Molenaars et al., 2020 ) (yeast, fruit flies, zebrafish and C. elegans) and culture medium. Additional details on these matrices can be found in Table S1 Sample Matrix Overview.…”

“…Human muscle biopsies were obtained as part of a previous study ( Grevendonk et al., 2021 ; Janssens et al., 2022 ). Research was approved by the institutional medical ethical committee, and experiments were conducted in agreement with the Declaration of Helsinki.…”

“…The study was registered at clinicaltrials.gov with identifier NCT03666013 . Data obtained for this study, using this protocol, has been published in Nature Aging by Janssens et al. (2022) .…”

“…Metabolomics data from these samples have not been made available here as these data do not belong to the Core Facility Metabolomics. They have been published by Janssens et al. (2022) .…”

Ratiometric NAD+ sensors reveal subcellular NAD+ modulators

“…Recently, low NAD + levels have been linked to multiple disease states, therefore NAD + -boosting approaches have been proposed as a potential therapeutic strategy against various diseases 2–4 . In fact, increasing evidences suggest that NAD + -boosting strategies can improve physiological and metabolic health in rodent models of ageing, obesity, and diabetes 5,6 , and recent clinical studies suggested a clear association between NAD + and physiological status during human aging 7 and disease progression 8 .…”