Health Surveillance of Specific Pathogen-Free and Conventionally-Housed Mice and Rats in Korea

Seok, Seung Hyeok; Park, Jonghwan H.; Cho, Suna; Baek, Min Won; Lee, Hui Young; Kim, Dongjae; Yang, Ki-Hwa; Jang, Dongdeuk; Han, Beom Seok; Nam, Kitaek; Park, Jae Hak

doi:10.1538/expanim.54.85

Cited by 10 publications

(11 citation statements)

References 24 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Data from Japan showed that 27 out of 47 (57.5%) sentinel mice obtained during 1998 and 1999 from universities, breeding companies, pharmaceutical companies, and national research institutions were contaminated with Helicobacter species (13). A Korean study revealed Helicobacter infection in only one out of 24 mouse strains that were housed in SPF facilities (29). A European study reported a prevalence of Helicobacter infection of 88.9% (eight of nine strains) in mice (25).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

et al. 2006

View full text Add to dashboard Cite

Infections with enterohepatic Helicobacter species (EHS) can change the results of animal experiments.However, there is little information about the prevalence of EHS in noncommercial animal facilities. The aim of this study was to investigate the prevalence and the spread of EHS in specific-pathogen-free (SPF) mice. Fecal samples of 40 mouse lines were analyzed for members of the family Helicobacteraceae using a group-specific PCR targeting the 16S rRNA gene. Additional experiments were carried out to evaluate the spread of EHS among mice harbored in different caging systems. Helicobacter species were detected in 87.5% of the mouse lines tested. Five different Helicobacter species were identified: H. ganmani, H. hepaticus, H. typhlonicus, and the putative Helicobacter species represented by the isolates hamster B and MIT 98-5357. Helicobacter infection did not spread between animals in neighboring cages when individually ventilated cages were used; in contrast, when the mice were reared in open-air cages, EHS were found to spread from cage to cage. However, the spread was prevented by adding polycarbonate filter tops to the cages. When Helicobacter-negative and infected mice shared the same cage, transmission of the infection occurred in 100% within 2 weeks. Furthermore, we found that mice from commercial breeding facilities may carry undetected Helicobacter infections. Taken together, we show that infection with EHS may frequently occur and spread easily in mice reared under SPF conditions despite extensive safety precautions. Moreover, there is a high prevalence of rather uncommon Helicobacter species that may be a consequence of the current routine procedures used for health screening of SPF mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

et al. 2006

View full text Add to dashboard Cite

show abstract

“…The most prevalent pathogen was MHV (Fig. 1), a very stubborn laboratory mouse pathogen in Korea which has been present for a long time [16,19]. Our data showed that 23.2% of tested facilities had MHV seropositive mouse, and MHV-infected mouse facilities with barrier systems account for 8.8% of the tested facilities.…”

Section: Discussionmentioning

confidence: 70%

“…The monitoring results of Specific Pathogen Free (SPF) and conventional mice and rats in Korea from 2000 to 2003, based on this monitoring method, were previously published based on this monitoring method [16]. In this study, we aimed to clarify the current microbiological states of research animals in Korea in the period covering 2006 to 2007, and to suggest some recommendations for the improvement of the quality of laboratory animals.…”

Section: Introductionmentioning

confidence: 99%

Microbiological Quality Assessment of Laboratory Mice in Korea and Recommendations for Quality Improvement

Seok

Lee

et al. 2010

Exp. Anim.

View full text Add to dashboard Cite

Regular monitoring of commercial laboratory rodents and institutional research animal residents is essential for microbiological quality control programs. The objective of our study was to investigate the recent prevalence of infectious pathogens in laboratory mice from eight experimental animal vendors and 56 institutional animal facilities in Korea. Our investigation was conducted in 2006-2007. Specific Pathogen Free (SPF) mice from four commercial breeders were clean according to serological, bacteriological, parasitological, and histopathological examination results. However, mice from one intermediate vendor that distributed SPF animals from main commercial vendors to local districts had Syphacia obvelata and Mycoptes musculinus infections. Additionally, mice from conventional animal breeders were highly contaminated. Among the 56 institutional animal facilities, mouse hepatitis virus (MHV), Sendai virus and Mycoplasma pulmonis positive results were obtained in 23.2, 8.9, and 1.8% of animals tested, respectively. These results indicate that quarantine and eradication efforts of infectious pathogens in these facilities are sub-optimal and need to be improved. The use of commercial conventional mice for research should be eliminated and appropriate vendor selection as well as thorough quarantine before releasing animals into a facility are needed. Finally we suggest qualified veterinary experts are needed at each animal facility to ensure an adequate health surveillance program.

show abstract

“…The contents of the large intestine were used for the detection of endoparasites and this experiment was performed according to the method used in our previous report [15].…”

Section: Examination For Endoparasitesmentioning

confidence: 99%

Microbiological Monitoring of Guinea Pigs Reared Conventionally at Two Breeding Facilities in Korea

et al. 2006

View full text Add to dashboard Cite

show abstract

Health Surveillance of Specific Pathogen-Free and Conventionally-Housed Mice and Rats in Korea

Cited by 10 publications

References 24 publications

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

Microbiological Quality Assessment of Laboratory Mice in Korea and Recommendations for Quality Improvement

Microbiological Monitoring of Guinea Pigs Reared Conventionally at Two Breeding Facilities in Korea

Contact Info

Product

Resources

About