Healing Segmental Femoral Defects in Sheep Using Recombinant Human Bone Morphogenetic Protein

Tissue engineered bone; Application for implant surgery

“…The results of this study provide evidence of the safety and technical feasibility of TEB for maxillary sinus floor augmentation and vertical ridge augmentation in agreement with those from earlier animal studies that have indicated that treatment with TEB dose not result in toxicity, significant immunologic reactions, or other serious adverse effects [20][21][22][23] .…”

Ex vivo gene therapy with stromal cells transduced with a retroviral vector containing the BMP4 gene completely heals critical size calvarial defect in rats

“…Several well-established critical size defect models have been used in the past for investigating the efficacy of biomaterials, growth factors and gene therapy vectors. 3,5,6 In this study, we used an 8-mm cranial defect model in rats to evaluate the efficacy of ex vivo gene therapy. Several lines of evidence demonstrate that stromal cells transduced with MLV-based BMP2/4 retroviral vector can completely heal the defect within 4 weeks.…”

“…1,2 The osteoinductive potential of BMPs, especially BMP2, BMP4 and BMP7, makes them clinically valuable as alternatives to bone grafts. [3][4][5][6] In order to promote the healing of fractures or the fillingin of large bone defects, the BMPs should be delivered at adequate concentrations for a period of time sufficient to induce a strong bone formation response. Delivery of the BMP gene into an osseous defect has the potential to induce prolonged local synthesis of BMPs with a single application.…”

Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery

“…The identification of BMPs has generated great interest due to their potential use in bone regeneration. 3 Several recombinant forms of BMPs, mostly rhBMP-2 and rhBMP-7 (a.k.a., OP-1), have been shown to induce bone formation in vivo, [13][14][15][16][17][18][19][20][21][22][23][24] and both rhBMP-2 and rhBMP-7 have been tested in clinical trials. [25][26][27] In addition to the direct application of recombinant BMP proteins, numerous reports have confirmed the ability of adenoviral or retroviral vector-mediated gene transfer of several BMPs to induce bone formation in animal models.…”