Healing patterns of critical size bony defects in rat following bone graft

Mokbel, Nadim; Serhal, C Bou; Matni, Ghassan; Naaman, Nada

doi:10.1007/s10006-008-0107-7

Cited by 38 publications

(37 citation statements)

References 25 publications

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“…The promotion of scaffolds or osteoconductive bone substitutes to enhance osseous regeneration in bone defects is well known [3,26,29]. In the current study, the extremely low-magnitude, high-frequency mechanical stimulus caused greater increases in bone area, volume, and thickness in scaffolded than in nonscaffolded defects.…”

Section: Discussionmentioning

confidence: 53%

Extremely Small-magnitude Accelerations Enhance Bone Regeneration: A Preliminary Study

Hwang

Lublinsky

Seo

et al. 2008

Clin Orthop Relat Res

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High-frequency, low-magnitude accelerations can be anabolic and anticatabolic to bone. We tested the hypothesis that application of these mechanical signals can accelerate bone regeneration in scaffolded and nonscaffolded calvarial defects. The cranium of experimental rats (n = 8) in which the 5-mm bilateral defects either contained a collagen scaffold or were left empty received oscillatory accelerations (45 Hz, 0.4 g) for 20 minutes per day for 3 weeks. Compared with scaffolded defects in the untreated control group (n = 6), defects with a scaffold and subject to oscillatory accelerations had a 265% greater fractional bone defect area 4 weeks after the surgery. After 8 weeks of healing (1-week recovery, 3 weeks of stimulation, 4 weeks without stimulation), the area (181%), volume (137%), and thickness (53%) of the regenerating tissue in the scaffolded defect were greater in experimental than in control animals. In unscaffolded defects, mechanical stimulation induced an 84% greater bone volume and a 33% greater thickness in the defect. These data provide preliminary evidence that extremely low-level, high-frequency accelerations can enhance osseous regenerative processes, particularly in the presence of a supporting scaffold.

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Section: Discussionmentioning

confidence: 53%

Extremely Small-magnitude Accelerations Enhance Bone Regeneration: A Preliminary Study

Hwang

Lublinsky

Seo

et al. 2008

Clin Orthop Relat Res

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show abstract

“…It is recognized that the critical size for a bone defect is 15 mm in length: without bone implantation or replacement, the bone defect cannot be self-repaired. 25 Thirty-six rabbits were randomly divided into two groups. Eighteen rabbits were put into each group depending on the different scaffolds used.…”

Section: Implantation Surgerymentioning

confidence: 99%

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Wang

He²,

Bi³

et al. 2013

IJN

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Purpose: Poly(lactic-co-glycolic acid) (PLGA) is excellent as a scaffolding matrix due to feasibility of processing and tunable biodegradability, yet the virgin scaffolds lack osteoconduction and osteoinduction. In this study, nano-hydroxyapatite (nHA) was coated on the interior surfaces of PLGA scaffolds in order to facilitate in vivo bone defect restoration using biomimetic ceramics while keeping the polyester skeleton of the scaffolds. Methods: PLGA porous scaffolds were prepared and surface modification was carried out by incubation in modified simulated body fluids. The nHA coated PLGA scaffolds were compared to the virgin PLGA scaffolds both in vitro and in vivo. Viability and proliferation rate of bone marrow stromal cells of rabbits were examined. The constructs of scaffolds and autogenous bone marrow stromal cells were implanted into the segmental bone defect in the rabbit model, and the bone regeneration effects were observed. Results: In contrast to the relative smooth pore surface of the virgin PLGA scaffold, a biomimetic hierarchical nanostructure was found on the surface of the interior pores of the nHA coated PLGA scaffolds by scanning electron microscopy. Both the viability and proliferation rate of the cells seeded in nHA coated PLGA scaffolds were higher than those in PLGA scaffolds. For bone defect repairing, the radius defects had, after 12 weeks implantation of nHA coated PLGA scaffolds, completely recuperated with significantly better bone formation than in the group of virgin PLGA scaffolds, as shown by X-ray, Micro-computerized tomography and histological examinations. Conclusion: nHA coating on the interior pore surfaces can significantly improve the bioactivity of PLGA porous scaffolds.

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“…[6][7][8] However, such grafts have yet to be applied clinically due to donor site limitations, morbidity, and…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

Du¹,

Liu²,

Deng³

et al. 2015

IJN

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To improve the regenerative performance of nano-hydroxyapatite/coralline (nHA/coral) block grafting in a canine mandibular critical-size defect model, nHA/coral blocks were coated with recombinant human vascular endothelial growth factor 165 (rhVEGF) via physical adsorption (3 μg rhVEGF 165 per nHA/coral block). After the nHA/coral blocks and VEGF/nHA/coral blocks were randomly implanted into the mandibular box-shaped defects in a split-mouth design, the healing process was evaluated by histological observation and histomorphometric and immunohistological analyses. The histological evaluations revealed the ingrowth of newly formed blood vessels and bone at the periphery and cores of the blocks in both groups at both 3 and 8 weeks postsurgery, respectively. In the histomorphometric analysis, the VEGF/nHA/coral group exhibited a larger quantity of new bone formation at 3 and 8 weeks postsurgery. The percentages of newly formed bone within the entire blocks in the VEGF/nHA/coral group were 27.3%±8.1% and 39.3%±12.8% at 3 weeks and 8 weeks, respectively, and these values were slightly greater than those of the nHA/coral group (21.7%±3.0% and 32.6%±10.3%, respectively), but the differences were not significant ( P >0.05). The immunohistological evaluations revealed that the neovascular density in the VEGF/nHA/coral group (146±32.9 vessel/mm 2 ) was much greater than that in the nHA/coral group (105±51.8 vessel/mm 2 ) at the 3-week time point ( P <0.05), but no significant difference was observed at the 8-week time point (341±86.1 and 269±50.7 vessel/mm 2 , respectively, P >0.05). The present study indicated that nHA/coral blocks might be optimal scaffolds for block grafting in critical-size mandibular defects and that additional VEGF coating via physical adsorption can promote angiogenesis in the early stage of bone healing, which suggests that prevascularized nHA/coral blocks have significant potential as a bioactive material for bone regeneration in large-scale alveolar defects.

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Healing patterns of critical size bony defects in rat following bone graft

Cited by 38 publications

References 25 publications

Extremely Small-magnitude Accelerations Enhance Bone Regeneration: A Preliminary Study

Extremely Small-magnitude Accelerations Enhance Bone Regeneration: A Preliminary Study

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Angiogenesis and bone regeneration of porous nano-hydroxyapatite/coralline blocks coated with rhVEGF165 in critical-size alveolar bone defects in vivo

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