Healing of osteoporotic bone defects by micro-/nano-structured calcium phosphate bioceramics

Zhao, Rui; Chen, Siyu; Yuan, Bo; Chen, Xuening; Yang, Xi; Song, Yueming; Tang, Hai; Yang, Xiao; Zhu, Xiangdong; Zhang, Xingdong

doi:10.1039/c8nr09417a

Cited by 41 publications

(47 citation statements)

References 67 publications

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“…In osteoporotic pathological conditions, osteogenesis is seriously impaired, mainly due to decreased osteoblast activity (49). In our previous studies, we proved that nHA promotes the bone-forming function of osteoblasts through the upregulation of endoplasmic reticulum calcium ATPase (adenosine triphosphatase) 2 subunits ATP2A2 and ATP2B2 (30,31). We also compared the osteoporotic bone regeneration ability of bioceramics with 10% Sr content to that of strontium ranelate drug administration (35).…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we used an osteoblastic cell model that was primarily derived from osteoporotic animal bone explants, which we believe would better reflect cellular responsivity under pathological conditions. The osteoporotic bone explant culture protocol was established earlier (30,31). In contrast to the healthy osteoblasts derived from sham animals, osteoporotic bone-originated osteoblasts showed significantly lower ALP activity and matrix mineralization ability.…”

Section: Discussionmentioning

confidence: 99%

“…Later, we applied this nHA coating onto bioceramic and porous titanium scaffold by vacuum dipping and low-temperature sintering. The layer of nHA coating resulted in a large amount of mature new bone formation, which eventually enhanced the osteointegration of coated implants under osteolytic conditions caused by osteoporosis or bone tumor (31,32).…”

Section: Introductionmentioning

confidence: 99%

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A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

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Osteoporotic fractures are prevalent in society, and their incidence appears to be increasing as the worldwide population ages. However, conventional bone repair materials hardly satisfy the requirements for the repair of pathological fractures. Here, we developed a biomimetic polyetherketoneketone scaffold with a functionalized strontium-doped nanohydroxyapatite coating for osteoporotic bone defect applications. The scaffold has a hierarchically porous architecture and mechanical strength similar to that of osteoporotic trabecular bone. In vitro and in vivo studies demonstrated that the scaffold could promote osteoporotic bone regeneration and delay adjacent bone loss via regulating both osteoblasts and osteoclasts. In addition, the correlations between multiple preimplantation and postimplantation parameters were evaluated to determine the potential predictors of in vivo performance of the material. The current work not only develops a promising candidate for osteoporotic bone repair but also provides a viable approach for designing other functional biomaterials and predicting their translational value.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

et al. 2020

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“…9,11 Our recent investigations also indicate that HA nanoparticles and the resulting coating can enhance the osteogenic capability of porous implants. 12,13 Vascularization is considered among the important factors influencing the repairing of large bone defects. Blood vessels play a key role in transporting nutrients and maintaining the normal metabolism of the tissue.…”

Section: Introductionmentioning

confidence: 99%

<p>Effects of Nanotopography Regulation and Silicon Doping on Angiogenic and Osteogenic Activities of Hydroxyapatite Coating on Titanium Implant</p>

Xi¹,

Pin²,

Zhao³

et al. 2020

IJN

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Background: Angiogenic and osteogenic activities are two major problems with biomedical titanium (Ti) and other orthopedic implants used to repair large bone defects. Purpose: The aim of this study is to prepare hydroxyapatite (HA) coatings on the surface of Ti by using electrochemical deposition (ED), and to evaluate the effects of nanotopography and silicon (Si) doping on the angiogenic and osteogenic activities of the coating in vitro. Materials and Methods: HA coating and Si-doped HA (HS) coatings with varying nanotopographies were fabricated using two ED modes, ie, the pulsive current (PC) and cyclic voltammetry (CV) methods. The coatings were characterized through scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS), and atomic force microscopy (AFM), and their in vitro bioactivity and protein adsorption were assessed. Using MC3T3-E1 preosteoblasts and HUVECs as cell models, the osteogenic and angiogenic capabilities of the coatings were evaluated through in vitro cellular experiments. Results: By controlling Si content in~0.8 wt.%, the coatings resulting from the PC mode (HA-PC and HS-PC) and CV mode (HA-CV and HS-CV) had nanosheet and nanorod topographies, respectively. At lower crystallinity, higher ionic dissolution, smaller contact angle, higher surface roughness, and more negative zeta potential, the HS and PC samples exhibited quicker apatite deposition and higher BSA adsorption capacity. The in vitro cell study showed that Si doping was more favorable for enhancing the viability of the MC3T3-E1 cells, but nanosheet coating increased the area for cell spreading. Of the four coatings, HS-PC with Si doping and nanosheet topography exhibited the best effect in terms of up-regulating the expressions of the osteogenic genes (ALP, Col-I, OSX, OPN and OCN) in the MC3T3-E1 cells. Moreover, all leach liquors of the surfacecoated Ti disks promoted the growth of the HUVECs, and the HS samples played a more significant role in promoting cell migration and tube formation than the HA samples. Of the four leach liquors, only the two HS samples up-regulated NO content and expressions of the angiogenesis-related genes (VEGF, bFGF and eNOS) in the HUVECs, and the HS-PC yielded a better effect. Conclusion: The results show that Si doping while regulating the topography of the coating can help enhance the bone regeneration and vascularization of HA-coated Ti implants.

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“…Transcriptomics has been also used to study osteogenesis in the presence of different bone substitutes [ 104 ] and to characterize the osseointegration process in osteoporotic conditions. [ 105 ]…”

Section: Omics Approaches To Characterize the Bone Regeneration Procementioning

confidence: 99%

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

Calciolari

Donos

2020

Proteomics Clinical Apps

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Bone regeneration is a complex biological process, where the molecular mechanisms are only partially understood. In an ageing population, where the prevalence of chronic diseases with an impact on bone metabolism is increasing, it becomes crucial to identify new strategies that would improve regenerative outcomes also in medically compromised patients. In this context, omics are demonstrating a great potential, as they offer new insights on the molecular mechanisms regulating physiologic/pathologic bone healing and, at the same time, allow the identification of new diagnostic and therapeutic targets. This review provides an overview on the current evidence on the use of transcriptomic and proteomic approaches in bone regeneration research, particularly in relation to type 1 diabetes and osteoporosis, and discusses future scenarios and potential benefits and limitations on the integration of multi-omics. It is suggested that future research will leverage the synergy of omics with statistical modeling and bioinformatics to prompt the understanding of the biology underpinning bone formation in health and medically compromised conditions. With an eye toward personalized medicine, new strategies combining the mining of large datasets and bioinformatic data with a detailed characterization of relevant phenotypes will need to be pursued to further the understanding of disease mechanisms.

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Healing of osteoporotic bone defects by micro-/nano-structured calcium phosphate bioceramics

Abstract: The micro-/nano-structured calcium phosphate bioceramic exhibited a higher new bone substitution rate in an osteoporotic bone defect rat model.

Cited by 41 publications

References 67 publications

A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair

<p>Effects of Nanotopography Regulation and Silicon Doping on Angiogenic and Osteogenic Activities of Hydroxyapatite Coating on Titanium Implant</p>

Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions

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