Healing of Ischemic Colon Anastomosis in Rats Could Be Provided by Administering Dexpanthenol or Coenzyme Q10

Abstract

Background: In this study, the effectiveness of dexpanthenol and coenzyme Q10 (CoQ10) on the healing of ischemic colon anastomosis was investigated. Methods: Forty eight male Wistar Albino rats were divided into four equal groups (Sham-S, Sham-I, DXP, Q10). Following full layer colon resection, single layer colon anastomosis, without creating ischemia, was performed on the Sham-S group. The same experimental model was performed on remaining groups after ischemia was created. Intraperitoneal dexpanthenol and CoQ10 was administered to the DXP and Q10 groups once a day for three days. Ten days later, all colon anastomoses were investigated histopathologically and biochemically, as well as their burst pressure values, in all sacrificed rats. Results: The highest burst pressure value was observed in the Sham-S group, decreasing from high to low in the DXP, Q10, and Sham-I groups, respectively (p = 0.008). Furthermore, tissue hydroxyproline (p = 0.001) level values were significantly different among the groups. Additionally, histopathological analysis revealed a significant difference among groups regarding reepithelization (p = 0.027) and polymorphonuclear leukocyte density (p = 0.022). Conclusions: This preliminary study has shown that ischemia-reperfusion injury may impair the healing of colon anastomosis and it has been concluded that dexpanthenol and CoQ10 may have positive effects on the healing of ischemic colon anastomosis in rat, although re-epithelization may be adversely affected using CoQ10.

Different Surgical Methods in Colon Anastomosis: Experimental Study

“…Therefore, anastomotic leak has been one of the most investigated topics in colorectal surgery 11 , 24 . In the literature, there are experimental studies on anastomotic healing which involve methods such as reducing the number of sutures, dexpanthenol, coenzyme Q10, krill oil, and fish oil 3 , 6 , 9 , 17 . The indication for surgery and many factors related to the operated patient is also as effective as the technique used in colorectal surgery.…”

The effect of carbon monoxide releasing molecule-2 (CORM-2) on healing of ischemic colon anastomosis in rats

“…The latter is one of the important factors affecting the healing process [3]. Ischemia in the anastomosis may occur due to arteriosclerotic vascular disease, advanced age, inflammatory vascular disease, vasoconstrictor drug use, and vascular network damage during the surgical manipulation [4,5].…”