Healing of Artificial Fenestration Defects by Seeding of Fibroblast-Like Cells Derived from Regenerated Periodontal Ligament in a Dog: A Preliminary Study

Abstract

The purpose of this study was to assess the seeding of fibroblast-like cells to promote periodontal healing in artificial fenestration defects in a dog. Fibroblast-like cells were cultured by incubating regenerated periodontal ligament tissue, that had been surgically taken, underneath a Teflon membrane. Fenestration defects were surgically induced on the maxillary canine and first molar teeth at a spacing of 5 to 5 mm. Passage 4 cells (2 x 10(5) cells) in autologous blood coagulum were placed on root surfaces in two defects; the remaining two defects were used as controls. Healing was evaluated histomorphometrically on postoperative day 42. The main periodontal healing pattern consisted of connective tissue adaptation in three of the four specimens including one control, with cementum formation at 9-12%; one control specimen that exhibited 100% cementum formation. New bone formation was greater in the cell-seeding group (84%) compared with control (39%). In the cell-seeding group, one specimen exhibited total regeneration of bone (100%); however, the connective tissue located between newly formed bone and the root surface was observed to adapt to the dentin surface, with limited cementum formation. Seeding of cells from periodontal ligament may be promising to promote periodontal regeneration, but needs to be investigated in further studies.

Periodontal tissue regeneration by transplantation of rat adipose-derivedstromal cells in combination with PLGA-based solid scaffolds

“…In addition, the scaffold must be able to maintain a space within the wound site for the cells to grow into (5). Although using soft scaffolds such as hydro gels or collagen sponge has been the predominant strategy (8,20,29,42,54,64), the applications of these soft scaffolds are limited due to their poor mechanical properties. Solid scaffolds such as hydroxyapatite/tricalcium phosphate (HA/TCP) provide improved support for periodontal tissue regeneration (7,33).…”

Craniofacial Tissue Engineering by Stem Cells

“…Transplantation of cells derived from the periodontal ligament has showed the potential to regenerate periodontal attachment structures in vivo (Lekic et al, 2001;Dogan et al, 2003;Nakahara et al, 2004;Akizuki et al, 2005;Hasegawa et al, 2005). Cementoblasts or tooth-lining cells have a marked ability to induce mineralization in an ex vivo model (Jin et al, 2003b) and in vivo in periodontal wounds .…”

Current Status and Future Development of Cell Transplantation Therapy for Periodontal Tissue Regeneration

“…Previous studies that reported the regenerative properties of PDL using animal models indicated the existence of stem cells in PDL tissue [3, 4]. Liu et al reported that autologous PDL-MSCs enhanced regeneration of periodontal tissue, including alveolar bone, cementum and PDL in a minipig [5].…”