Healing Efficacy of an EGF Impregnated Triple Gel Based Wound Dressing: In Vitro and In Vivo Studies

Khanbanha, Najmeh; Atyabi, Fatemeh; Taheri, Azade; Talaie, Fatemeh; Mahbod, Mirgholamreza; Dinarvand, Rassoul

doi:10.1155/2014/493732

Cited by 32 publications

(19 citation statements)

References 36 publications

(33 reference statements)

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“…This suggested that the developed film has longer residence time on the wound site especially in suppurating wound accredited to its deformation resistant [42]. As reported by Khanbanha et al [49], the higher the viscosity value of a hydrocolloid film dressing, the better the dressing at maintaining the integrity of a drug delivery device.…”

Section: Discussionmentioning

confidence: 67%

Healing Effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and Development VCN-2 Hydrocolloid Film Based on Alginate as Potential Wound Dressing

Tan

Arulselvan

et al. 2020

BioMed Research International

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Chronic wounds represent serious globally health care and economic issues especially for patients with hyperglycemic condition. Wound dressings have a predominant function in wound treatment; however, the dressings for the long-lasting and non-healing wounds are still a significant challenge in the wound care management market. Astonishingly, advanced wound dressing which is embedded with a synthetic drug compound in a natural polymer compound that acts as drug release carrier has brought about promising treatment effect toward injured wound. In the current study, results have shown that Vicenin-2 (VCN-2) compound in low concentration significantly enhanced cell proliferation and migration of HDF. It also regulated the production of pro-inflammatory cytokines such as IL-6, IL-1β, and TNF-α from HDF in wound repair. Treatment of VCN-2 also has facilitated the expression of TGF-1β and VEGF wound healing maker in a dose-dependent manner. A hydrocolloid film based on sodium alginate (SA) incorporated with VCN-2 synthetic compound which targets to promote wound healing particularly in diabetic condition was successfully developed and optimized for its physico-chemical properties. It was discovered that all the fabricated film formulations prepared were smooth, translucent, and good with flexibility. The thickness and weight of the formulations were also found to be uniform. The hydrophilic polymer comprised of VCN-2 were shown to possess desirable wound dressing properties and superior mechanical characteristics. The drug release profiles have revealed hydrocolloid film, which is able to control and sustain the VCN-2 released to wound area. In short, hydrocolloid films consisting of VCN-2 formulations are suitably used as a potential wound dressing to promote restoration of wound injury.

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Section: Discussionmentioning

confidence: 67%

Healing Effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and Development VCN-2 Hydrocolloid Film Based on Alginate as Potential Wound Dressing

Tan

Arulselvan

et al. 2020

BioMed Research International

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“…Since LY has a positive charge, the surface potential of the LY shell exceeds zero, and thus it can attract molecules with negative charges. Therefore, the LY shell with positive charges can be adsorbed onto EGF that has a negative charge at pH values above 4.6 (which is the isoelectric pH of EGF) by electrical adsorption 29 , 30 . LYMBs were prepared according to the procedure used in our previous study 25 .…”

Section: Methodsmentioning

confidence: 99%

Ultrasound-Mediated EGF-Coated-Microbubble Cavitation in Dressings for Wound-Healing Applications

Liao

Hung

Chen

et al. 2018

Sci Rep

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The feasibility of ultrasound (US) controlled cavitation for transdermal drug delivery (TDD) using gas-filled microbubbles (MBs) has been explored. However, liquid or gel-type MBs is not easy used for TDD. The present study investigated a new treatment model for evaluating the US-mediated liquid-type epidermal growth factor (EGF)-coated lysozyme microbubble (LYMB) cavitation in a wound dressing for enhancing wound healing. The maximum loading efficacy of EGF onto LYMBs was 19.40 ± 0.04%. In terms of the in vitro treatment efficacy, the growth of Staphylococcus aureus was inhibited by 97.50 ± 1.50% in the group with LYMBs exposed to 3 W/cm2 US. During 21 days in vivo wound healing experiments, the recovery rate during the first 6 days was significant higher in the group with EGF-LYMB dressings and US exposure (day 6: 54.28 ± 3.26%) than in the control group (day 6: 26.36 ± 3.34%) (p < 0.05). Our results show that the new model can significantly reduce the treatment duration during wound healing.

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“…Previous studies have described new drugs that can promote wound healing without infection and decrease scar formation. [5][6][7][8] Epidermal growth factor (EGF) is secreted by platelets, monocytes and fibroblasts, and stimulates epidermal cells, fibroblasts, smooth muscle cells and endothelial cells to promote wound granulation and angiogenesis. Recombinant human epidermal growth factor (rhEGF) has been reported to improve wound healing in diabetic foot ulcer and radiation-related oral mucositis without major side effects.…”

Section: Introductionmentioning

confidence: 99%

Outcomes of the Recombinant Human Epidermal Growth Factor Addition to Chloramphenicol Ointment for Facial Burn Wound Healing

Namviriyachote

2019

Smj

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Objective: Delayed healing of facial burns can result in scarring and psychological morbidity. Epidermal growth factor may promote wound granulation and angiogenesis to enhance wound healing. We compared the effect of Chloramphenicol ointment alone with Recombinant Human Epidermal Growth Factor (rhEGF) plus chloramphenicol on facial burn wound healing. Methods: A randomized controlled trial was conducted in patients admitted to the Burn Unit. Subjects aged 18 to 65 years with acute second-degree facial burn wounds that did not require surgical treatment were enrolled. Subjects were divided equally and randomized to receive either topical chloramphenicol ointment twice daily (control) or rhEGF ointment once daily and chloramphenicol ointment twice daily. Wounds were assessed at frequent intervals. Wound size, complete healing day, pain score, infection, side effects, Vancouver Scar Scale and the cost of treatment were recorded. Results: Twenty-six wounds were enrolled. The mean wound size was similar in both groups (rhEGF plus chloramphenicol treated group 38.5±18.2 cm 2 vs control group 42.1±19.4 cm 2). Burn wounds in the rhEGF group healed more rapidly, though the difference was not statistically significant (8.5 ± 3.4 days vs 9.3 ± 4.4 days, p=0.3). No difference was observed in the Vancouver scar scale. Mean post-treatment pain scores were the same in both groups. There were no infections or side effects in either group. The rhEGF-treated group was more expensive (1463.1 ± 142.6 vs 19.2 ± 1.9 baht). Conclusion: There are no difference in outcomes of acute facial burn wound healing treated with rhEGF and chloramphenical ointment compared to chloramphenicol treatment alone. The further study to evaluate the effect of rhEGF in patients who have some factors that delay wound healing should be done.

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Healing Efficacy of an EGF Impregnated Triple Gel Based Wound Dressing: In Vitro and In Vivo Studies

Cited by 32 publications

References 36 publications

Healing Effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and Development VCN-2 Hydrocolloid Film Based on Alginate as Potential Wound Dressing

Healing Effect of Vicenin-2 (VCN-2) on Human Dermal Fibroblast (HDF) and Development VCN-2 Hydrocolloid Film Based on Alginate as Potential Wound Dressing

Ultrasound-Mediated EGF-Coated-Microbubble Cavitation in Dressings for Wound-Healing Applications

Outcomes of the Recombinant Human Epidermal Growth Factor Addition to Chloramphenicol Ointment for Facial Burn Wound Healing

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