Healing and expression of growth factors (TGF-β and PDGF) in canine radial ostectomy gap containing platelet-rich plasma

Souza, Thales R.; Andrade, Alexandre Lima de; Ferrreira, G. T. N. M.; Sakamoto, Silmara Sanae; Albuquerque, Verônica Batista de; Bonfim, Suely Regina Mogami; Luvizotto, Maria Cecília Rui; Louzada, Mário Jefferson Quirino

doi:10.3415/vcot-10-10-0146

Cited by 25 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite the popularity and advantages of PRP, there are conflicting reports in the literature regarding its efficacy [9–14, 16]. These differences may in part be attributable to variations in the composition of PRP preparations with respect to platelet yield, concentrations of beneficial growth factors and the presence of other blood components, particularly leukocytes [17–19].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

2017

View full text Add to dashboard Cite

BackgroundPlatelet-rich plasma (PRP) has been expected for regenerative medicine because of its growth factors. However, there is considerable variability in the recovery and yield of platelets and the concentration of growth factors in PRP preparations. The aim of this study was to identify optimal relative centrifugal force and spin time for the preparation of PRP from canine blood using a double-centrifugation tube method.MethodsWhole blood samples were collected in citrate blood collection tubes from 12 healthy beagles. For the first centrifugation step, 10 different run conditions were compared to determine which condition produced optimal recovery of platelets. Once the optimal condition was identified, platelet-containing plasma prepared using that condition was subjected to a second centrifugation to pellet platelets. For the second centrifugation, 12 different run conditions were compared to identify the centrifugal force and spin time to produce maximal pellet recovery and concentration increase. Growth factor levels were estimated by using ELISA to measure platelet-derived growth factor-BB (PDGF-BB) concentrations in optimised CaCl2-activated platelet fractions.ResultsThe highest platelet recovery rate and yield were obtained by first centrifuging whole blood at 1000 g for 5 min and then centrifuging the recovered platelet-enriched plasma at 1500 g for 15 min. This protocol recovered 80% of platelets from whole blood and increased platelet concentration six-fold and produced the highest concentration of PDGF-BB in activated fractions.ConclusionsWe have described an optimised double-centrifugation tube method for the preparation of PRP from canine blood. This optimised method does not require particularly expensive equipment or high technical ability and can readily be carried out in a veterinary clinical setting.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1123-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

2017

View full text Add to dashboard Cite

show abstract

“…[8, 25–27] One study showed a benefit in a radial gap model based upon radiographic and histologic assessments. [26] They used a population of dogs that was homogenous in body weight but varied in breed and there was no reporting on age of the animals or the effect of age on bone healing. [26] Conversely, another study failed to find a beneficial effect of PRP in combination with calcium phosphate granules in an ulnar defect in six beagles.…”

Section: Discussionmentioning

confidence: 99%

“…[26] They used a population of dogs that was homogenous in body weight but varied in breed and there was no reporting on age of the animals or the effect of age on bone healing. [26] Conversely, another study failed to find a beneficial effect of PRP in combination with calcium phosphate granules in an ulnar defect in six beagles. [25, 26] A third study presumably failed to identify a benefit of PRP combined with hydroxyapatite in a radial gap stabilized with bone plates.…”

Section: Discussionmentioning

confidence: 99%

“…[26] Conversely, another study failed to find a beneficial effect of PRP in combination with calcium phosphate granules in an ulnar defect in six beagles. [25, 26] A third study presumably failed to identify a benefit of PRP combined with hydroxyapatite in a radial gap stabilized with bone plates. [26, 27] Given these discrepant results the effects of different PRPs on long bone healing in dogs has remained unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Further, the lack of a gap probably mitigates identification of enhanced bone regeneration, which can be visualized more easily within a gap or defect. [26] Likewise, we obtained no histologic or mechanical data to assess the effects of PRP as these were client-owned dogs. Although these latter outcomes measures may increase the possibility of detecting a significant difference between groups they are not clinically applicable, whether in dogs or people.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of platelet-rich plasma on osseous healing in dogs undergoing high tibial osteotomy

2017

View full text Add to dashboard Cite

ObjectivesThe purpose of this study was to investigate whether platelet-rich plasma (PRP) enhances osseous healing in conjunction with a high tibial osteotomy in dogs.Study designRandomized controlled trial.MethodsSixty-four client-owned pet dogs with naturally occurring rupture of the anterior cruciate ligament and that were to be treated with a high tibial osteotomy (tibial plateau leveling osteotomy) were randomized into the treatment or control group. Dogs in the treatment group received autologous platelet-rich plasma activated with calcium chloride and bovine thrombin to produce a well-formed PRP gel that was placed into the osteotomy at the time of surgery. Dogs in the control group received saline lavage of the osteotomy. All dogs had the osteotomy stabilized with identical titanium alloy implants and all aspects of the surgical procedure and post-operative care were identical among dogs of the two groups. Bone healing was assessed at exactly 28, 49, and 70 days after surgery with radiography and ultrasonography and with MRI at day 28. The effect of PRP on bone healing was assessed using a repeated measures analysis of covariance with radiographic and ultrasonographic data and using a t-test with the MRI data.ResultsSixty dogs completed the study. There were no significant differences in age, weight, or gender distribution between the treatment and control groups. Twenty-seven dogs were treated with PRP and 33 were in the control group. The average platelet concentration of the PRP was 1.37x106 platelets/μL (±489x103) with a leukocyte concentration of 5.45x103/μL (±3.5x103). All dogs demonstrated progressive healing over time and achieved clinically successful outcomes. Time since surgery and patient age were significant predictors of radiographic healing and time since surgery was a significant predictor of ultrasonographic assessment of healing. There was no significant effect of PRP treatment as assessed radiographically, ultrasonographically, or with MRI.ConclusionThe PRP used in this study did not hasten osseous union in dogs treated with a high tibial osteotomy.

show abstract

Biological Therapies in Canine Sports Medicine

Franklin¹,

Pozzi²,

Steffen³

2018

Canine Sports Medicine and Rehabilitation

View full text Add to dashboard Cite

Healing and expression of growth factors (TGF-β and PDGF) in canine radial ostectomy gap containing platelet-rich plasma

Abstract: Platelet-rich plasma can be used as an adjuvant therapy because it may promote better bone healing of a radial ostectomy treated with external skeletal fixation in dogs.

Cited by 25 publications

References 23 publications

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

Optimisation of a double-centrifugation method for preparation of canine platelet-rich plasma

The effect of platelet-rich plasma on osseous healing in dogs undergoing high tibial osteotomy

Biological Therapies in Canine Sports Medicine

Contact Info

Product

Resources

About