Healing activity induced by Cramoll 1,4 lectin in healthy and immunocompromised mice

Abstract

Cramoll 1,4 is a lectin extracted from seeds of Cratylia mollis Mart. Many assays have shown the cytokine release activity and pro-inflammatory profile of this lectin. Here, we used Cramoll 1,4 in the treatment of cutaneous wounds in normal and immunocompromised mice for available your cicatricial power. Surgical wounds were treated daily with a topical administration of Cramoll 1,4 and parameters as edema, hyperemia, scab, granulation and scar tissues as well as contraction of wounds were analyzed. Cramoll 1,4 wounds showed higher edema and arrival of more polimorphonuclear cells at the site of lesions. Granulation tissue and collagen fiber deposition were observed with higher intensity in all Cramoll 1,4 treated wounds and promoted excellent closing and repair of lesions in less time than other groups. Results showed that Cramoll 1,4 lectin was effective in the repair of experimental lesions in mice and can be used as a future cicatricial compound.

Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models

“…In general, the animals treated with isolates and fractions of plants presented an elevated closure rate of the wound, representing 72.72% of the studies [17, 18, 20, 23–27, 29, 30, 33, 35–46], increase in tissue reepithelialization (30.3%) [15, 18–20, 23, 27, 35, 38, 43], increase of the traction strength on the cicatricle tissue (75.75%) [15, 17–20, 22–25, 27, 31, 33–46], greater content and organization of the extracellular matrix on fast expansion of the granulation tissue (42.42%)  [17, 18, 23, 30, 32, 33, 36, 38–40, 42, 44–46], and stimulation of the activity of endogenous antioxidant enzymes (9.09%) [16, 21, 22] (Tables 2(a) and 2(b)).…”

<i>In Vitro</i> Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

“…We evaluated the effects on the repair of cutaneous lesions in normal mice using the isoforms 1 and 4 of Cratylia mollis lectin [32] and in immunosuppressed animals by the use of methotrexate, a study by Melo et al [33], which had their injuries repaired efficiently and in less time.…”

Lectins, Interconnecting Proteins with Biotechnological/Pharmacological and Therapeutic Applications

“…In order to analyze the cicatricial power, the lectin was applied to topical treatment of cutaneous wounds surgically induced in normal and immunocompromised albino Swiss mice [128]. Treated animals showed more edema formation and recruitment of more polymorphonuclear cells at the wounds.…”

“…Treated animals showed more edema formation and recruitment of more polymorphonuclear cells at the wounds. The prevalence of polymorphonuclear cells induced by Cramoll 1,4 is important to remove cell debris and microorganisms in the wound, favoring the healing [128]. Cramoll 1,4 also induced efficiently the granulation phase, collagen fiber deposition, and no incidence of microorganisms in all treated wounds, resulting in wound closure and repair faster than control groups.…”