Healing activities of Cramoll and xyloglucan membrane in cutaneous wounds of diabetic mice

Andrade, Fernanda Miguel de; Neves, Fernanda P.A.; Albuquerque, Priscilla Barbosa Sales de; Aragão-Neto, Adelmo C.; Jandú, Jannyson José Braz; Coelho, Luana Cassandra Breitenbach Barroso; Lima-Ribeiro, Maria Helena Madruga; Teixeira, Álvaro A.C.; Carneiro-da-Cunha, Maria G.; Teixeira, Valéria Wanderley; Correia, Maria T.S.

doi:10.1016/j.regen.2021.100045

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…used in biosensors due to its protein relevance in biological systems [13,14] . Cramoll has the properties of healing wounds, [15] repairing skin wounds in diabetics, [16] detecting arbovirus glycoprotein expression, [17] characterizing malignant tumors, [18,19] among other applications.…”

Section: Introductionmentioning

confidence: 99%

Biosensor Characterization from Cratylia mollis Seed Lectin (Cramoll)‐MOF and Specific Carbohydrate Interactions in an Electrochemical Model

Carvalho

Araújo

Silva

et al. 2022

Chemistry & Biodiversity

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Biosensors are small devices known for their selectivity, high specificity and sensitivity to the respective analyte, at low concentrations. We developed an electrochemical biosensor using the crystalline polymer MOF-[Cu 3 (BTC) 2 (H 2 O) 2 ] n to characterize Cratylia mollis seed lectin (Cramoll) and its interaction with free carbohydrate (glucose) and carbohydrates on the surface of rabbit erythrocytes. The electrochemical potentials presented by the exponential curves that vary from 96 to 142 mV in relation to concentrations of 10 to 20 mM of glucose are decisive for the use of the system containing gold electrode/MOF/Cramoll for the characterization of biological models due to its high sensitivity. As well as the kinetic behavior presented in the cyclic voltammograms, with a cathodic current response of 0.000 3 A for a glucose concentration of 15 mM. These results were due to the high specificity of Cramoll under these conditions, promoting stability of surface charges at the Cramoll/electrode interface. This phenomenon facilitates the monitoring of the interaction with free glucose present in the electrolyte medium by potentiometric and amperometric methods and with carbohydrates present on the surface of rabbit erythrocytes through the potentiometric method. Through scanning electron microscopy (SEM) it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose-lectin) through the morphological lectin changes in this process. This electrochemical model, Cramoll/ MOF biosensor, is effective for evaluating free lectin/carbohydrate or in the erythrocyte membrane.

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