Headspace Solid-Phase Microextraction Analysis of Volatile Components in Narcissus tazetta var. chinensis Roem

Chen, Hsin-Chun; Chi, Hai-Shan; Lin, Li-Yun

doi:10.3390/molecules181113723

Cited by 25 publications

(22 citation statements)

References 20 publications

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“…This difference is most likely due to the different plant species being tested, since, the extraction time depends on the chemical nature of the compounds present, the distribution constant, the fiber polymeric phase, and to the size of the molecular mass (e.g., polyunsaturated fatty acids and other compounds are expected to require longer extraction times depending on their lower partitioning and diffusion coefficient). The HS-SPME method has been used for extracting volatile compounds from flowers at room temperature, this method is favorable for flower VOCs analysis, because it minimizes high temperature artifacts [34]. In the present study, the results indicated that there were differences between intact and cut flower for the Floribunda cultivar, and intact flowers were superior to excised flower by total peak area.…”

Section: Discussionsupporting

confidence: 46%

Optimized Method to Analyze Rose Plant Volatile Organic Compounds by HS-SPME-GC-FID/MSD

Ibrahim¹,

Agarwal²,

Hardy³

et al. 2017

JBM

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A method involving Headspace solid-phase microextraction (HS-SPME) fiber combined with gas chromatography (GC) coupled with flame ionization detection (FID) and gas chromatography with mass spectrometry (GC-MS) was developed and optimized to investigate volatile organic compounds (VOCs) from different tissues (flowers, leaves, stems, rhizosphere and whole plants) of Floribunda and Hybrid Tea roses (intact and cut). Three-phase fiber 50/30 µm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) was used. Two types of chambers (Tedlar bag and glass jar) were evaluated for collection of VOCs and glass jar was selected. Absorbed compounds on the fiber were completely desorbed in the GC injector port at three desorption times (5, 10 and 15 min), and 5 min at 250˚C was used. The maximum extraction efficiency for flowers tissues (equilibrium absorption) was achieved 2 h after fiber exposure in the headspace for intact and cut Floribunda and Hybrid Tea flowers. Under the optimized HS-SPME and GC-FID/MS conditions, 1h extraction time was chosen for intact and cut Floribunda and Hybrid Tea leaves and stems. The results demonstrated that 5 cm depth was selected for root and soil part (rhizosphere) for both rose cultivars, and 6 h and 12 h extraction time of VOCs from rhizosphere was achieved for Floribunda and Hybrid Tea, respectively. One hour was chosen for VOCs released from whole rose plants for both cultivars. In this study, the VOC profiles of two rose cultivars were characterized by the optimized HS-SPME-GC method. The different tissues of rose plants gave wide range of the VOCs; also the chromatograms of different cultivars were quite different and the specific VOC pattern of rose types depends on the species. Results from this study demonstrate the feasibility of this method for identifying VOCs from two rose cultivars and the potential use of this method for physiological studies on rose plants or on other floriculture plants.

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Section: Discussionsupporting

confidence: 46%

Optimized Method to Analyze Rose Plant Volatile Organic Compounds by HS-SPME-GC-FID/MSD

Ibrahim¹,

Agarwal²,

Hardy³

et al. 2017

JBM

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“…The volatile compounds were analyzed by Iglesias et al 17 . During the sampling time, the fiber was inserted into the headspace of the reaction vessel.…”

Section: Volatile Compound Analysismentioning

confidence: 99%

Characterization of Volatile Compounds with HS-SPME from Oxidized n-3 PUFA Rich Oils via Rancimat Tests

et al. 2017

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“…The typical methods used include liquid–liquid extraction, purge and trap, steam distillation (SD), simultaneous distillation extraction (SDE), supercritical-fluid extraction (SFE), solid-phase extraction (SPE), and solid-phase microextraction (SPME) [ 15 ]. SPME is simple, fast, sensitive, requires no solvent [ 16 ], and has been successfully used to analyze volatile compounds in a variety of flowering plants, such as Hosta , Tree peony, Styrax tonkinensis , Narcissus tazetta , and Phalaenopsis [ 8 , 15 , 17 , 18 , 19 ]. Many of these floral fragrance volatiles are terpenes, phenylpropanoids, or fatty acid derivatives [ 5 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

The Variation of Oncidium Rosy Sunset Flower Volatiles with Daily Rhythm, Flowering Period, and Flower Parts

2017

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Oncidium is an important ornamental crop worldwide, and in recent years, the characteristics of the flower aroma have become a concern for breeders. This study used headspace solid-phase microextraction (HS-SPME) and gas chromatography/mass spectrometry (GC-MS) analysis of the volatile compounds to study the aroma characteristics of Onc. Rosy Sunset. A total of 45 compounds were identified, with the major compound being linalool. Onc. Rosy Sunset had the highest odor concentration from 10:00 to 12:00 and lowest from 20:00 to 24:00. The inflorescence emitted the highest quantities of volatile compounds during stages 3–6, which then decreased with the aging of the flowers. In Onc. Rosy Sunset, the sepals and petals were the major parts for the floral fragrance emission, in which linalool content was the highest, whereas the lip and column had a different composition of major volatile compounds, of which benzaldehyde, β-myrcene, and β-caryophyllene dominated.

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Headspace Solid-Phase Microextraction Analysis of Volatile Components in Narcissus tazetta var. chinensis Roem

Cited by 25 publications

References 20 publications

Optimized Method to Analyze Rose Plant Volatile Organic Compounds by HS-SPME-GC-FID/MSD

Optimized Method to Analyze Rose Plant Volatile Organic Compounds by HS-SPME-GC-FID/MSD

Characterization of Volatile Compounds with HS-SPME from Oxidized n-3 PUFA Rich Oils via Rancimat Tests

The Variation of Oncidium Rosy Sunset Flower Volatiles with Daily Rhythm, Flowering Period, and Flower Parts

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