Headspace Solid-Phase Microextraction Analysis of Volatile Components in Phalaenopsis Nobby’s Pacific Sunset

Abstract

Phalaenopsis is the most important economic crop in the Orchidaceae family. There are currently numerous beautiful and colorful Phalaenopsis flowers, but only a few species of Phalaenopsis have an aroma. This study reports the analysis volatile components present in P. Nobby's Pacific Sunset by solid-phase microextraction (SPME) coupled with gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS). The results show that the optimal extraction conditions were obtained by using a DVB/CAR/PDMS fiber. A total of 31 compounds were identified, with the major compounds being geraniol, linalool and α-farnesene. P. Nobby's Pacific Sunset had the highest odor concentration from 09:00 to 13:00 on the eighth day of storage. It was also found that in P. Nobby's Pacific Sunset orchids the dorsal sepals and petals had the highest odor concentrations, whereas the column had the lowest.

The Variation of Oncidium Rosy Sunset Flower Volatiles with Daily Rhythm, Flowering Period, and Flower Parts

“…These results were similar to those reported for Phal. Nobby’s Pacific Sunset and Gongora bufonia, which showed stronger odor levels at 09:00–13:00 and 10:00, respectively [ 8 , 22 ]. Orchids possess entomophilous flowers, and the aroma volatile components are key for the attraction of insects.…”

“…Floral fragrance is one of the important features of the ornamental orchid, and can improve the aesthetic value, quality of flower products, and their economic merit. Cultivating fragrance is a current trend in orchid breeding, with several studies involving orchid volatile compound analysis and synthesis, mainly focused on Phalaenopsis [ 4 , 5 , 6 , 7 , 8 ]. There are few fragrant cultivars of Oncidium at present, with the minimal research into their aromatic volatile compounds mostly confined to local journals and focused on Onc.…”

“…The typical methods used include liquid–liquid extraction, purge and trap, steam distillation (SD), simultaneous distillation extraction (SDE), supercritical-fluid extraction (SFE), solid-phase extraction (SPE), and solid-phase microextraction (SPME) [ 15 ]. SPME is simple, fast, sensitive, requires no solvent [ 16 ], and has been successfully used to analyze volatile compounds in a variety of flowering plants, such as Hosta , Tree peony, Styrax tonkinensis , Narcissus tazetta , and Phalaenopsis [ 8 , 15 , 17 , 18 , 19 ]. Many of these floral fragrance volatiles are terpenes, phenylpropanoids, or fatty acid derivatives [ 5 , 13 , 14 ].…”

Floral fragrances ofHemerocallisL. (daylily) evaluated by headspace solid-phase microextraction with gas chromatography-mass spectrometry

“…The identification of floral volatiles involved comparison of their mass spectra with a NIST 2011 mass spectral library with a G1711EA MSD ChemStation (Agilent) and by comparing their Kovats retention indices (RI), which were calculated by the use of a series of n-alkane standards (C7-C19) (Accu Standard, New Haven, CT, USA), with related data from the literature (Dormont, Delle-Vedove, Bessière, & Schatz, 2014;Gong, Chen, Liu, Dunn, & Sun, 2014;Jabalpurwala, Smoot, & Rouseff, 2009;Jürgens, Glück, Aas, & Dötterl, 2014;Kishimoto et al, 2013;Kite & Leon, 1995;Pino, Marbot, & Vázquez, 2001;Yeh et al, 2014). Additionally, some authentic reference standards [myrcene and ocimene (TCI, Shanghai, China); linalool (Tokyo Chemical Industry Corporation, Tokyo, Japan)] were used.…”

An Analysis of Volatile Components of the Liverworts Dumortiera hirsuta subsp. hirsuta and Dumortiera hirsuta subsp. nepalensis (Dumortieraceae) from Panama and Taxonomic Observations on the Species

“…Headspace solid phase microextraction (HS-SPME) is a simple, fast, and sensitive method that allows for extraction of volatile compounds present in plants by using a minimal amount of sample [11]. This technique coupled with gas chromatography-mass spectrometry (GC-MS) has been widely used for the analysis of VOcs and for determination of chemical markers in plants [11][12][13][14][15], as well as in a vast diversity of bryophytes [6].…”