Headspace gas chromatographic determination of distribution coefficients of selected organosulphur compounds and their dependence on some parameters

Przyjazny, Andrzej; Janicki, W.; Chrzanowski, Wojciech; Staszewski, R.

doi:10.1016/s0021-9673(00)91567-x

Cited by 119 publications

(57 citation statements)

References 11 publications

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“…Headspace specific activity was 21.9 + 1.7 mCi mmol-L (n = 5). Because DMS has a partition coefficient of approximately 10 at typical room temperatures (Przyjazny et al 1983, Dacey et al 1984, the headspace radioactivity was approximately 0.1 nCi y1-l, and injection volumes were 20 to 500 p1 into 60 m1 water samples, for final concentrations of 1.4 to 35 nM I4C-DMS.…”

Section: Radioisotope Incubations Ul[14c]dms ((I4ch3)?s)mentioning

confidence: 99%

Radioisotope and chemical inhibitor measurements of dimethyl sulfide consumption rates and kinetics in estuarine waters

Wolfe¹,

Kiene²

1993

Mar. Ecol. Prog. Ser.

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Microbial consumption rates of 1 to 20 nM dimethyl sulfide (DMS) in estuanne wholewater samples from coastal Georgia (USA) were measured by 2 methods production of radiolabeled CO2 and particulates >O 2 pm from ('4CH,)2S, and specific inhibition by addition of 500 FM chloroform The combination and companson of these 2 methods helped overcome some inherent limitations of each one, and both methods gave short turnover times (< 1 d ) at low DMS concentrations (1 to 3 nM) DMS production was often as rapid as its consumption, suggesting very tight cycling However, when exogenous DMS was added to increase concentrations, consumption rates saturated below 20 nM, suggesting high affinities for DMS Kinehcs and rates appeared s m d a r over a several week penod in samples taken from the Duplin River, a coastal tidal nver where DMS concentrations were consistently in the range of 1 to 3 nM In contrast, samples of water whlch had recently flooded the marsh had much higher DMS concentrations, but consumption rates were simllar to those observed in the creek waters before they flooded the marsh The turnover tlmes calculated for the waters over the marsh were on the order of 3 to 7 d The radioisotope method showed that DMS assinillation into cell matenal was approximately equal to that respired as CO2, and confirmed 500 pM CHC13 as an excellent inhibitor of DMS consumption However it appears that chloroform may in some cases increase dissolved DMSP concentrations and hence DMS production, leading to overestimates of DMS consumption rates Two other inhibitors of DMS consumption, dimethyl disulfide (100 nM) and dimethyl ether (30 PM), gave similar but lower consumption rates compared to chloroform, and show promise as specif~c inhibitors for future studies

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Section: Radioisotope Incubations Ul[14c]dms ((I4ch3)?s)mentioning

confidence: 99%

Radioisotope and chemical inhibitor measurements of dimethyl sulfide consumption rates and kinetics in estuarine waters

Wolfe¹,

Kiene²

1993

Mar. Ecol. Prog. Ser.

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“…In our previous biofiltration study, the specific growth rate of the microbial community was experimentally determined to be 0.012 h Ϫ1 (49). Assuming an airwater partition coefficient of 0.07 for DMS (28), the approximate aqueous concentration of DMS in our batch studies is 300 M while the approximate concentration of DMS in the biofilm of our biofilters, with an inlet DMS concentration of 20 parts per million by volume (ppmv), would be approximately 10 M. The half-velocity constant, K S , of the biofilter microbial community was determined to be 0.2 M (49), but even if the biofilter microbial community had a K S on DMS similar to that of Hyphomicrobium VS (3 M) (27), it would be expected that the specific growth rate at pH 7 would be approximately 0.07 h Ϫ1 , not 0.012 h Ϫ1 , which is a value that is more analogous to the specific growth rate at pH 5 in the batch studies. In other words, the pH conditions in these biofilters resulted in much lower specific growth rates than can be achieved by Hyphomicrobium spp.…”

Section: Discussionmentioning

confidence: 99%

Growth Kinetics of Hyphomicrobium and Thiobacillus spp. in Mixed Cultures Degrading Dimethyl Sulfide and Methanol

Hayes

Liss

Allen

2010

Appl Environ Microbiol

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The growth kinetics of Hyphomicrobium spp. and Thiobacillus spp. on dimethyl sulfide (DMS) and methanol (in the case of Hyphomicrobium spp.) in an enrichment culture created from a biofilter cotreating DMS and methanol were studied. Specific growth rates of 0.099 h ؊1 and 0.11 h ؊1 were determined for Hyphomicrobium spp. and Thiobacillus spp., respectively, growing on DMS at pH 7. These specific growth rates are double the highest maximum specific growth rate for bacterial growth on DMS reported to date in the literature. When the pH of the medium was decreased from pH 7 to pH 5, the specific growth rate of Hyphomicrobium spp. decreased by 85%, with a near 100-fold decline in the yield of Hyphomicrobium 16S rRNA gene copies in the mixed culture. Through the same pH shift, the specific growth rate and 16S rRNA gene yield of Thiobacillus spp. remained similar. When methanol was used as a substrate, the specific growth rate of Hyphomicrobium spp. declined much less over the same pH range (up to 30%) while the yield of 16S rRNA gene copies declined by only 50%. Switching from an NH 4 ؉ -N-based source to a NO 3 ؊ -N-based source resulted in the same trends for the specific growth rate of these microorganisms with respect to pH. This suggests that pH has far more impact on the growth kinetics of these microorganisms than the nitrogen source. The results of these mixed-culture batch experiments indicate that the increased DMS removal rates observed in previous studies of biofilters cotreating DMS and methanol are due to the proliferation of DMS-degrading Hyphomicrobium spp. on methanol at pH levels not conducive to high growth rates on DMS alone.Dimethyl sulfide (DMS) is a reduced sulfur compound that is emitted from both natural and anthropogenic sources. Natural DMS emissions are largely the result of the cleavage of dimethylsulfoniopropionate (9), the breakdown of the sulfurcontaining amino acids methionine and cysteine (9, 11), and the degradation of methoxylated aromatic compounds (3, 9). Anthropogenic DMS emissions tend to be the result of hightemperature industrial processes and are problematic due to the foul smell of DMS and its low odor threshold (34). Industries that are sources of anthropogenic DMS emissions include wastewater treatment (14), aerobic composting (40), animal rendering (23), and kraft pulping (35).In the environment, microbial degradation can be a significant sink for DMS. In seawater, approximately 90% of the DMS produced is removed biologically before it reaches the atmosphere (21). Removal of DMS in the environment can be carried out by a variety of pathways. Aerobic bacteria, such as Hyphomicrobium spp. The prevalence of bacteria in the environment capable of growth on DMS has created interest in developing low-cost biotechnological methods to remove DMS from industrial waste gas streams. One possible technology is biofiltration which involves passing waste air through a packed bed of microorganisms. Removal of DMS in these systems, however, has proved to be difficult. This is believed t...

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“…The column temperature was programmed from 60 • C (1 min isothermal) to 240 • C (2 min isothermal) at a rate of 40 • C min −1 . The distribution coefficients for DMS and MT at 55 • C are 6.9 and 4.3, respectively (Przyjazny et al, 1983). Calibration was made with standards prepared anoxically from chemicals.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Microbial conversion of inorganic carbon to dimethyl sulfide in anoxic lake sediment (Plußsee, Germany)

et al. 2010

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Abstract. In anoxic environments, volatile methylated sulfides like methanethiol (MT) and dimethyl sulfide (DMS) link the pools of inorganic and organic carbon with the sulfur cycle. However, direct formation of methylated sulfides from reduction of dissolved inorganic carbon has previously not been demonstrated. When studying the effect of temperature on hydrogenotrophic microbial activity, we observed formation of DMS in anoxic sediment of Lake Plußsee at 55 • C. Subsequent experiments strongly suggested that the formation of DMS involves fixation of bicarbonate via a reductive pathway in analogy to methanogenesis and engages methylation of MT. DMS formation was enhanced by addition of bicarbonate and further increased when both bicarbonate and H 2 were supplemented. Inhibition of DMS formation by 2-bromoethanesulfonate points to the involvement of methanogens. Compared to the accumulation of DMS, MT showed the opposite trend but there was no apparent 1:1 stoichiometric ratio between both compounds. Both DMS and MT had negative δ 13 C values of −62‰ and −55‰, respectively. Labeling with NaH 13 CO 3 showed more rapid incorporation of bicarbonate into DMS than into MT. The stable carbon isotopic evidence implies that bicarbonate was fixed via a reductive pathway of methanogenesis, and the generated methyl coenzyme M became the methyl donor for MT methylation. Neither DMS nor MT accumulation were stimulated by addition of the methyl-group donors methanol and syringic acid or by the methyl-group acceptor hydrogen sulphide. The source of MT was further investigated in a H 35 2 S labeling experiment, which demonstrated a microbially-mediated process of hydrogen sulfide methyCorrespondence to: Y. S. Lin (yushih@uni-bremen.de) lation to MT that accounted for only <10% of the accumulation rates of DMS. Therefore, the major source of the 13 C-depleted MT was neither bicarbonate nor methoxylated aromatic compounds. Other possibilities for isotopically depleted MT, such as other organic precursors like methionine, are discussed. This DMS-forming pathway may be relevant for anoxic environments such as hydrothermally influenced sediments and fluids and sulfate-methane transition zones in marine sediments.

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Headspace gas chromatographic determination of distribution coefficients of selected organosulphur compounds and their dependence on some parameters

Cited by 119 publications

References 11 publications

Radioisotope and chemical inhibitor measurements of dimethyl sulfide consumption rates and kinetics in estuarine waters

Radioisotope and chemical inhibitor measurements of dimethyl sulfide consumption rates and kinetics in estuarine waters

Growth Kinetics of Hyphomicrobium and Thiobacillus spp. in Mixed Cultures Degrading Dimethyl Sulfide and Methanol

Microbial conversion of inorganic carbon to dimethyl sulfide in anoxic lake sediment (Plußsee, Germany)

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