Head-to-head comparison of diagnostic accuracy of four Ebola virus disease rapid diagnostic tests versus GeneXpert® in eastern Democratic Republic of the Congo outbreaks: a prospective observational study

Mukadi-Bamuleka, Daniel; Bulabula-Penge, Junior; Jacobs, Bart K M; Weggheleire, Anja De; Edidi-Atani, François; Mambu-Mbika, Fabrice; Legand, Anaïs; Klena, John D.; Fonjungo, Peter N.; Mbala-Kingebeni, Placide; Makiala-Mandanda, Sheila; Kajihara, Masahiro; Takada, Ayato; Formenty, Pierre; Muyembe‐Tamfum, Jean‐Jacques; Ariën, Kevin K.; Griensven, Johan van; Ahuka‐Mundeke, Steve; Kavunga-Membo, Hgo; Ishara-Nshombo, Elie; Rogé, Stijn; Mulopo-Mukanya, Noella; Tsiwedi-Tsilabia, Espérance; Muhindo-Milonde, Emile; Kavira-Muhindo, Marie-Anne; Morales-Betoulle, Maria; Nkuba-Ndaye, Antoine

doi:10.1016/j.ebiom.2023.104568

Cited by 4 publications

(1 citation statement)

References 24 publications

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“…It was deployed during the 2018-2022 outbreak of EVD in the Democratic Republic of the Congo with a reported sensitivity and specificity of 85% and 99.8% [18] and 87.4% and 99.6%, respectively [45]. Indeed, QuickNavi TM -Ebola showed comparable performance to other WHO-approved RDTs [18,45,46]. One limitation of the present study is that a positive IC assay test could not be related to the onset of viremia due to the 3-day intervals between the collection of the infected NHP sera, unlike our previous study on QuickNavi TM -Ebola where the collection was at shorter intervals and the majority of NHPs tested positive upon the onset of viremia [17].…”

Section: Discussionmentioning

confidence: 99%

Development of an Immunochromatography Assay to Detect Marburg Virus and Ravn Virus

Changula,

Kajihara,

Muramatsu

et al. 2023

Viruses

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The recent outbreaks of Marburg virus disease (MVD) in Guinea, Ghana, Equatorial Guinea, and Tanzania, none of which had reported previous outbreaks, imply increasing risks of spillover of the causative viruses, Marburg virus (MARV) and Ravn virus (RAVV), from their natural host animals. These outbreaks have emphasized the need for the development of rapid diagnostic tests for this disease. Using monoclonal antibodies specific to the viral nucleoprotein, we developed an immunochromatography (IC) assay for the rapid diagnosis of MVD. The IC assay was found to be capable of detecting approximately 102−4 50% tissue culture infectious dose (TCID50)/test of MARV and RAVV in the infected culture supernatants. We further confirmed that the IC assay could detect the MARV and RAVV antigens in the serum samples from experimentally infected nonhuman primates. These results indicate that the IC assay to detect MARV can be a useful tool for the rapid point-of-care diagnosis of MVD.

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