Head-to-head agglutination of ram and boar epididymal spermatozoa and evidence for an epididymal antagglutinin

Dacheux, J.L.; Paquignon, M.; Combarnous, Yves

doi:10.1530/jrf.0.0670181

Cited by 48 publications

(30 citation statements)

References 24 publications

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“…However, occurrence of sperm agglutination has not been observed in the epididymal duct of these regions, although many spermatozoa have already acquired the capacity for head-to-head agglutination [7]. It is evident that at least 2 kinds of anti-agglutinins are included in boar epididymal fluid.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis.

Harayama

Miyano

Masuda³

et al. 1995

Journal of Reproduction and Development

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Section: Discussionmentioning

confidence: 97%

“…Slightly prior to these occurrences, spermatozoa show similar changes in head-to-head agglutination [7]. Namely, the capacity for headto-head agglutination appears in cells passing through the distal caput to the proximal corpus and is subsequently reduced by the time they reach the cauda.…”

mentioning

confidence: 97%

Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis.

Harayama

Miyano

Masuda³

et al. 1995

Journal of Reproduction and Development

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“…In fact, in mature sperm it fails to respond to a wide range of regulators, including fluoride [67, 731, forskolin [48, 1271, and many hormones commonly known to activate adenyl cyclase [38,29, 731. Sperm adenyl cyclase is activated, however, by MnZ+ and Mg2+ [18, 67,73, 1061.…”

Section: External Regulation Of Camp Levelsmentioning

confidence: 99%

Regulation of Mammalian Sperm Motility

Lindemann

Kanous

1989

Archives of Andrology

124

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The physiological regulation of sperm motility has become more amenable to investigation since the demonstration that CAMP and calcium play a role in modulating the functioning of the flagellar axoneme. Although the external triggering mechanisms that initiate motility and capacitation are still unknown, evidence supports a modification of the calcium balance by gated Ca2+ channels, accompanied by shifts in the internal pH. Ca2' and pH may in turn act indirectly through CAMP and CAMPdependent kinase (kinase,) to control the phosphorylation state of functional proteins in the flagellar axoneme. The role of calcium is of central importance, but it is clear that several separate Ca2+-dependent mechanisms are involved. Ca2+ controls the curvature of the sperm flagellum and, so, can change the motility of the sperm from progressive swimming to tumbling. Under the appropriate conditions, calcium appears to have the capacity to deactivate motility by activating phosphodiesterase and phosphatase. The deactivating effect of Ca2+ may be offset under some circumstances by coactivation of adenyl cyclase, so phosphorylation of the axoneme and the motility are maintained. The specific factors determining the predominant calcium effect are not yet known, but internal pH of the sperm may play a major role.Key Words: Sperm motility; Mammalian sperm; Mammalian reproduction; Flagellum; Calcium; pH. INTRODUCTIONThis review explores the influences regulating motility in mammalian sperm. Sperm motility depends on a flagellum, the organelle that develops the propulsive force for swimming. The cellular signals that modulate the action of the flagellar motor apparatus are the primary focus of this survey. Evidence is also examined to define the role of the male and female reproductive tracts in controlling sperm motility. To facilitate this review, structure/function relationships in mammalian sperm flagellum are briefly discussed. For the most part, the information included in this review has been obtained from research studies on mammalian sperm to identify the regulatory mechanisms most likely to be of physiological importance in mammalian reproduction. Received and accepted January 24, 1989. MORPHOLOGICAL PARAMETERSMammalian sperm swim by the propulsive action of a single flagellum. The structural foundation of the mammalian sperm flagellum is the 9 + 2 arrangement of microtubules that extends centrally through its entire length. This microtubule arrangement, called the axoneme, is common to all eukaryotic flagella ( Fig. 1) and also to cilia. Surrounding the axoneme are larger fibers, called coarse fibers or outer dense fibers (ODF), that are paired with the 9 outer doublet tubules. These fibers are anchored to the base of the flagellum in a caplike structure known as the connecting piece (CP). From there they taper in diameter along the flagellar length, terminating one-half to three-quarters of the way down the flagellum. The sperm mitochondria are wrapped around the basal one-third of the flagellum and this section is referred ...

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“…tabolism [7], location of a cytoplasmic droplet [6], capacity to undergo the acrosome reaction [8], ability to bind tightly to the zona pellucida [9] and to the egg plasma membrane [5,8], head-to-head agglutinability [5,10,11], polypeptide components [12][13][14] and so on. However, few attempts have been made so far to reveal maturation-dependent changes in the lectin affinity for spermatozoa in boars, although the detailed lectin-binding characteristics of sperm components as well as of sperm oar spermatozoa gradually become mature during epididymal transit and then are stored in B the cauda epididymidis until ejaculation [1,2].…”

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confidence: 99%

“…However, few attempts have been made so far to reveal maturation-dependent changes in the lectin affinity for spermatozoa in boars, although the detailed lectin-binding characteristics of sperm components as well as of sperm oar spermatozoa gradually become mature during epididymal transit and then are stored in B the cauda epididymidis until ejaculation [1,2]. The sperm maturation is believed to result from the exposure of luminal spermatozoa to the regionspecific environment within the epididymal duct produced by epithelial secretion and absorption [3,4] and, includes changes in motility [5,6], me-surface has been examined by the lectin blotting technique after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) [15,16], the analysis via fluorescence or electron microscopy [17,18] and the flow cytometric analysis [19] in other species (e.g. rams, rats and mice).…”

mentioning

confidence: 99%

Lectin-Binding Characteristics of Extracts from Epididymal Boar Spermatozoa.

Harayama

Watanabe²,

Masuda³

et al. 1998

Journal of Reproduction and Development

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Abstract. Glycosylated components of boar spermatozoa and epididymal plasma from the central caput, distal corpus and distal cauda epididymides were compared by electrophoretic and lectin blotting techniques. The four lectin reagents used in this study were Concanavalin A (Con A), Lens Culinaris Agglutinin (LCA), Ricinus Communis Agglutinin I (RCA I) and Peanut Agglutinin (PNA), which were labeled with peroxidase. The affinity of Con A and of LCA increased for three bands and one smear (A: >86.8 kDa, B: 80 kDa, C: 65-75 kDa and D: 45 kDa) and for two bands and one smear (A, B and C) of extracts from spermatozoa, respectively, as they passed through the epididymis. RCA I apparently recognized one band and one smear (G: >86.8 kDa and H: 46-58 kDa) of extracts from the distal cauda spermatozoa, though it hardly or faintly reacted with those from the central caput and distal corpus spermatozoa. PNA showed a similar affinity for band "G" to RCA I. Moreover, bands and smears with similar mobility (A, B, C, D, G and H) were also detected in epididymal plasma by lectin blotting techniques. On the other hand, several sperm components were detected more visibly with Con A (E: 37-41 kDa and F: 32-37 kDa), RCA I (I: 41 kDa) and PNA (I: 41 kDa and J: 47-51 kDa) in sperm extracts from the distal cauda than in those from the central caput, though no corresponding components were observed in epididymal plasma. These epididymal maturation-dependent modifications related to glycosylation in boar spermatozoa are discussed.

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Head-to-head agglutination of ram and boar epididymal spermatozoa and evidence for an epididymal antagglutinin

Cited by 48 publications

References 24 publications

Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis.

Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis.

Regulation of Mammalian Sperm Motility

Lectin-Binding Characteristics of Extracts from Epididymal Boar Spermatozoa.

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