Head &amp; neck optical diagnostics: vision of the future of surgery

Upile, Tahwinder; Jerjes, Waseem; Sterenborg, Henricus J. C. M.; El‐Naggar, Adel K.; Sandison, Ann; Witjes, Max J. H.; Biel, Merrill A.; Bigio, Irving J.; Wong, Brian J. F.; Gillenwater, Ann M.; MacRobert, Alexander J.; Robinson, Dominic J.; Betz, Christian; Stepp, Herbert; Bolotine, Lina; McKenzie, Gordon; Mosse, Charles A.; Barr, Hugh; Chen, Zhongping; Berg, Kristian; D’Cruz, Anil; Stone, Edwin M.; Kendall, Catherine; Fisher, Sheila; Leunig, Andreas; Olivo, Malini; Richards‐Kortum, Rebecca; Soo, Khee Chee; Bagnato, Vanderlei Salvador; Choo-Smith, Lin Ping; Svanberg, Katarina; Tan, I. Bing; Wilson, Brian C.; Wolfsen, Herbert C.; Yodh, Arjun G.; Hopper, Colin

doi:10.1186/1758-3284-1-25

Cited by 36 publications

(47 citation statements)

References 15 publications

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“…When the molecule relaxes it emits a photon and it returns to a different rotational or vibrational state. The difference in energy between the original state and this new state leads to a shift in the emitted photon's frequency away from the excitation wavelength [54][55][56]. The reported sensitivity of this technique was of 80.5% and specificity of 86.2% [59].…”

Section: Raman Spectroscopymentioning

confidence: 90%

“…The probe of the system should be in contact with the tissues, and no light is collected from the surface reflections. Absorption characteristic provides the optical signature of the tissue [54][55][56]. Lovat et al stated that the sensitivity was 92% and specificity was 60% and it differentiated high risk sites from inflammation with a sensitivity and specificity of 79% [60].…”

Section: Elastic Scattering Spectroscopymentioning

confidence: 99%

“…Recent advances include the possibility to extract true spectra of single fluorophores (chemical compound that can reemit light upon light excitation) by mathematically eliminating the undesired influences of scattering and absorption. As well, tumour-specific enzymes are about to be specifically targeted by fluorescent markers "smart probes" in order to improve both sensitivity and specificity [54][55][56]. Ebenezar stated that the diagnostic algorithm based on discriminant function scores obtained by fluorescence excitation spectroscopy (FES) method was able to distinguish welldifferentiated squamous cell carcinoma from normal lesions with a sensitivity of 100% and specificity of 100% [57].…”

Section: Enhanced Dyementioning

confidence: 99%

“…The 5-aminolevulinic acid enhances protoporphyrin IX which fluoresces red after excitation with blue light. The same excitation results in green fluorescence of molecules such as NAD and FADH, which are depleted in high metabolic rate of malignant tissues [54][55][56]. Shin stated that the sensitivity of the fluorescence imaging techniques ranged from 60 to 97% and specificity from 75 to 99% [58].…”

Section: Ratio Imagingmentioning

confidence: 99%

“…It helps to obtain a vibrational spectroscopic picture of the tissue content, thus provide immediate real time histology [54][55][56]. The technique is used to observe vibrational, rotational, and other low-frequency modes in a system.…”

Section: Raman Spectroscopymentioning

confidence: 99%

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The Outline of Prognosis and New Advances in Diagnosis of Oral Squamous Cell Carcinoma (OSCC): Review of the Literature

Omar

2013

Journal of Oral Oncology

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Objective. Oral squamous cell carcinoma (OSCC) has a remarkable incidence over the world and a fairly strenuous prognosis, encouraging further research on the prognostic factors and new techniques for diagnosis that might modify disease outcome. Data Sources. A web-based search for all types of articles published was initiated using Medline/Pub Med, with the key words such as oral cancer, prognostic factors of oral cancer, diagnostic method of oral cancer, and imaging techniques for diagnosis of oral cancer. The search was restricted to articles published in English, with no publication date restriction (last update April, 2013). Review Methods. In this paper, I approach the factors of prognosis of OSCC and the new advances in diagnostic technologies as well. I also reviewed available studies of the tissue fluorescence spectroscopy and other noninvasive diagnostic aids for OSCC. Results. The outcome is greatly influenced by the stage of the disease (especially TNM). Prognosis also depends or varies with tumour primary site, nodal involvement, tumour thickness, and the status of the surgical margins. Conclusion. Tumour diameter is not the most accurate when compared to tumour thickness or depth of invasion, which can be related directly to prognosis. There is a wide agreement on using ultrasound guided fine needle aspiration biopsies in the evaluation of lymph node metastasis.

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Section: Raman Spectroscopymentioning

confidence: 90%

Section: Elastic Scattering Spectroscopymentioning

confidence: 99%

Section: Enhanced Dyementioning

confidence: 99%

Section: Ratio Imagingmentioning

confidence: 99%

Section: Raman Spectroscopymentioning

confidence: 99%

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The Outline of Prognosis and New Advances in Diagnosis of Oral Squamous Cell Carcinoma (OSCC): Review of the Literature

Omar

2013

Journal of Oral Oncology

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Multimodal nonlinear microscopic investigations on head and neck squamous cell carcinoma: Toward intraoperative imaging

Meyer

Guntinas‐Lichius

Eggeling³

et al. 2012

Head & Neck

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Optical molecular imaging detects changes in extracellular pH with the development of head and neck cancer

Loja¹,

Luo²,

Farwell³

et al. 2012

Intl Journal of Cancer

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Non-invasive localized measurement of extracellular pH in cancer tissues can have a significant impact on the management of cancer. Despite its significance, there are limited approaches for rapid and non-invasive measurement of local pH in a clinical environment. In this study, we demonstrate the potential of noninvasive topical delivery of Alexa-647 labeled pHLIP (pH responsive peptide conjugated with Alexa Fluor® 647) to image changes in extracellular pH associated with head and neck squamous cell carcinoma using widefield and high resolution imaging. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of Alexa-647 labeled pHLIP in intact fresh human tissue specimens using widefield and high-resolution fluorescence imaging. Using topical delivery, Alexa-647 labeled pHLIP can be rapidly delivered throughout the epithelium of intact tissues with a depth exceeding 700 microns. Following labeling with Alexa-647 labeled pHLIP, the mean fluorescent contrast of increased 4 to 8 fold higher in clinically abnormal tissues as compared to paired clinically normal biopsies. Furthermore, the imaging approach showed significant differences in fluorescence contrast between the cancer and the normal biopsies across diverse patients and different anatomical sites (unpaired comparison). The fluorescence contrast differences between clinically abnormal and normal tissues were in agreement with the pathologic evaluation. Topical application of fluorescently labeled pHLIP can detect and differentiate normal from cancerous tissues using both widefield and high resolution imaging. This technology will provide an effective tool to assess tumor margins during surgery and improve detection and prognosis of head and neck cancer.

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Head & neck optical diagnostics: vision of the future of surgery

Cited by 36 publications

References 15 publications

The Outline of Prognosis and New Advances in Diagnosis of Oral Squamous Cell Carcinoma (OSCC): Review of the Literature

The Outline of Prognosis and New Advances in Diagnosis of Oral Squamous Cell Carcinoma (OSCC): Review of the Literature

Multimodal nonlinear microscopic investigations on head and neck squamous cell carcinoma: Toward intraoperative imaging

Optical molecular imaging detects changes in extracellular pH with the development of head and neck cancer

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