HDL Inhibited Atherosclerosis Induced by Radiation Injury

Abstract

Background- HDL inhibits atherosclerosis development from radiation damage; however, the mechanism for this process has not been fully defined. Methods- This study used radiation patients and cultured mouse aortic endothelial cells (MAECs) to investigate the process. Firstly, 158 patients from oncology department of Jingzhou hospital who have got radiation after neck cancers, the arterial function was monitored by B ultrasound, HDL and other blood lipid indexes were tested.Then, MAECs were isolated and cultured, MTT assay was used to test the HDL protective role on UVB radiation, along with this, western blotting was proceed to test some apotosis protein expression and possible molecular.Results- Firstly, those patients with high HDL levels were less likely to develop atherosclerosis, with statistical differences. We observed that MAECs treated with UVB was damaged significantly; HDL reversed the cell damage as dose-depended manner. At mean time, the apoptosis process was assessed and found that HDL inhibited the apoptosis caused by UVB. Western blotting results showed that HDL enhanced phosphatidylinositol 3-kinase (PI3K) and Akt phosphorylation in MAECs.Conclusion- These results suggest that HDL protected UVB-mediated appotosis by activation of a mechanism involving PI3K/Akt signal pathway.